This study were performed in Tropical Plant Resources Research Center of Korea National Arboretum, Pocheon, Korea to assess the effects of beneficial insects against Pseudococcus comstocki (Kuwana) on Phyllanthus acidus (Linnaeus) Skeels and Tetranychus urticae Koch on Acalypha wilkesiana Muell Arg. We investigated the density of leaf-feeding P. comstocki at intervals of approximately 2 weeks and T. urticae at intervals of approximately 1 weeks after introducing their natural enemies; Chrysoperla nipponensis (Okamoto) and Phytoseiulus persimilis Athias-Henriot. The densities of P. comstocki were suppressed 80.0% (after 4 weeks) and 97.5% (after 8 weeks) after introducing C. nipponensis. The densities of T. urticae were suppressed 84.1% (after 4 weeks) and 99.6% (after 6 weeks) after introducing P. persimilis.

This study was carried out to obtain the information on the antioxidant activity of Phyllanthus. We compared and analyzed three indigenous collections of Phyllanthus: species from Korea, Brazil and Paraguay. Total phenolic acid contents were 1.93±0.015 mg/mL in KCS (Korean Collection Species), 1.65±0.003 mg/mL in PCS (Paraguay Collection Species) and 1.49±0.003 mg/mL in BCS(Brazil Collection Species). In both DPPH radical scavenging activities and SOD-liked activities, KCS was the highest (67.25±0.03%, 78.38±1.17%). KCS were also the highest in hydroxyl radical scavenging activities (95.80±0.17%). It turned out that there was no meaningful difference between BCS (81.64±0.23%) and PCS (81.54±0.04%). As for hydrogen radical scavenging activities, there was no statistically meaningful difference between KCS (36.44±0.24%) and BCS (36.31±0.33%) and it was the lowest in PCS (33.72±0.15%). Our results showed that KCS excelled the others not only in antioxidant activity but also in other beneficial effects. This encouraging results may be potentially useful in developing Phyllanthus urinaria L. variety which contains lots of functional elements such as total phenolic acid.

본 실험은 여우구슬의 기내 부정근 유도 및 증식조건의 확립을 목적으로 수행되었다. 우선 여우구슬의 기내 발아체로부터 부위를 달리하여 부정근을 유도한 결과 줄기부위는 뿌리보다 양호한 부정근의 유도를 보였다. 또한 유도된 부정근을 이용하여 옥신의 종류(IAA, IBA, NAA와 2.4-D)에 따른 부정근 유도율을 조사한 결과 IBA와 NAA는 IAA와 2.4-D보다 높은 유도율을 보였다. IBA의 농도에 따른 유도율과 증식효율은 IBA가 0.5 mg/L첨가되었을 때 가장 높은 유도 및 증식효율을 보였다. 최적의 액체배지조건을 확인하고자 IBA의 농도는 0.5 mg/L로 첨가하고 sucrose의 농도를 달리하여 실험한 결과 sucrose는 30 g/L 첨가 되었을 때 가장 높은 생중량과 건중량을 나타냈다. 액체배양된 여우구슬의 부정근을 각각 MS, 1/2MS, 1/3MS배지에 30 g/L sucrose, 0.5 mg/L IBA가 첨가된 5 L 용량의 생물반응기에 4주간 배양한 결과 1/2MS 배지에서 양호한 생장을 보였다. 본 실험에서는 여우구슬의 종자발아체를 이용하여 부정근의 유도 및 증식조건에 필요한 기내배양조건과 2차적으로 유도된 부정근을 이용하여 플라스크와 생물반응기 배양을 통한 효율적인 증식조건을 확립하였다.