There are three kinds of Pleurotus eryngii : P. eryngii var. eryngii (P. eryngii), P. eryngii var. ferula (P. ferula : A-Wei-Mo) P. eryngii var. nebrodensis (P. nebrodensis : Bai-Ling-Gu). We bred P. ferula using Di-Mon mating and physiological assay and selected as follow (1) ‘Beesan No.1’ produced high yields, (2) ‘Beesan No.2’ was excellent morphological shape and anti-adipogenic Activit, (3) ‘Ergo’ included high ergothioneine such as anto-oxidant material, (4) ‘GW10-45’ included highest ergothioneine such as anto-oxidant material, (5) ‘Maeksong’ included high ergothioneine and effect of anti-inflammatory. We suggest that there are able to food-medicine materials.

This study investigated physiological characteristics and genetic relationship of 30 strains of Lentinula edodes, collected from the Europe, Asia, North America and preserved in the Forest Mushroom Research Institute(FMRI). In physiological characteristics, Papua New Guinea strain was excellent mycelium growth in 25℃ for 7 days on PDA media. For all strains, the optimal temperature for mycelial growth, their tunicate and color of hypha were observed. It surveyed their mycelial growth on oak sawdust media in test tube and independence with inter-strains and cultivar developed in FMRI by strain's anastomosis culture. It was carried by RAPD using operon primers as molecular genetic methods, investigated genetic relationships among strains using UPGMA in NYSYSpc(2.1) according to the presence or absence of bands. <This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries>