This study was performed to investigate the possibility of repeated superovulation treatment at interval from 27 days to 41 days in Hanwoo cattle and to compare with superovulation effect between doses of FSH 200 mg and FSH 400 mg. Different doses of FSH (200 mg or 400 mg) were injected at Day 8 after controlled internal drug release (CIDR) treatment for superovulation of Hanwoo donors following CIDR treatment (Day 8 after the estrus). Superovulation was repeated four times for one donor and number of corpus luteum (CL), number of embryos, number of transferable embryos and pregnancy rate after embryo transfer (ET) were investigated. 5 cows were used for each FSH treatment (10 cows in total). Average number of CL were and for the donors treated with FSH 200mg and FSH 400mg, respectively. Average number of embryos collected were and for the donors treated with FSH 200 mg and FSH 400 mg, respectively. Average number of transferable embryos were and for the donors treated with FSH 200 mg and FSH 400 mg, respectively. The pregnancy rate following ET with embryos collected from 200 mg FSH treated donors and 400 mg FSH treated donors were 61.9% and 53.8% respectively. The numbers of embryos tended to be decreased as the numbers of repeat of superovulation were elapsed. These results indicated that superovulation treatment by about a month to Hanwoo donors is usable and 200 mg of FSH is preferable for simple FSH treatment following CIDR treatment.

본 연구는 재래산양에 있어서 과배란 처리시 공란 산양의 반복사용에 따른 oocyte의 회수방법과 회수란의 질적 개선방법을 확립하기 위하여 실시하였으며, 성선자극호르몬의 1회 처리와 반복처리, 호르몬제의 종류에 따른 oocyte의 회수율과 등급을 조사하였다. 공시동물은 체중 15~25kg 전후의 성숙한 미경산 재래산양으로서 발정 동기화를 위하여 CIDR를 10일간 질내에 삽입하고 과배란 처리는 70 mg의 FSH 와 1,000 IU의 PMSG를 투여하여

토끼에서는 수정란 이식과 같은 기본적인 번식공학적 방법의 효율성이 아직 생쥐와 같은 실험동물에 비해 떨어지고 있어 생물공학적인 기술을 응용하는데 큰 어려움이 있다. 특히 유전자 이식에 의한 형질전환 토끼의 생산과 같은 생물공학적인 기술을 실용화하는데 효율이 높은 수정란이식 기술의 개발이 필수적이라고 할 수 있다. 본 연구에서는 토끼에서 수정란 이식 기술의 첫 단계인 과배란 유도를 효율적으로 이용할 수 있는 방법을 정립하기 위해 반복적인 과배란 유도가 배

These studies were conducted to investigate the effect of repreated superovulation on embryo production, the effect of the frozen-thawed embryos transferred on the developmental stage and grade, and donor-recipient synchrony on pregnancy rate in Korean native cattle. The results obtained in these studies were as follows: 1. Repeated superovulations in Korean Native Catile were not affected on the number of corpus luteum (CL), embryos recovered and embryos cleaved (range: 4.8 4.21 to 9.5 6.50, 1.8 2.53 to 8.2 8.04 and 1.6 2.32 to 4.0 4.59, respectively). 2. Blastocyst embryos (38.5%) showed higher pregnancy rate than morula (31.6%). 3. The pregnancyrates of cattle transferred with good and fair embryos were 33.3% and 40.4%, respectively. 4. The pregnancy rate when the donors exhibited estrus 12 hours earlier than the recipients (62.5%) was higher than when the donors and recipients exhibited estrus at the same time (33.3%) or when the donors exhibited estrus 12 hours later than the recipients (20.0%).