Gut contents analysis is essential to predict the impact of organisms on food source changes due to variations of the habitat environment. Previous studies of gut content analysis have been conducted using traditional methods, such as visual observation. However, these studies are limited in analyzing food sources because of the digestive process in gut organ. DNA metabarcoding analysis is a useful method to analyze food sources by supplementing these limitations. We sampled marine fish of Pennahia argentata, Larimichthys polyactis, Crangon affinis, Loligo beka and Sepia officinalis from Gwangyang Bay and Yeosu fisheries market for analyzing gut contents by applying DNA metabarcoding analysis. 18S rRNA v9 primer was used for analyzing food source by DNA metabarcoding. Network and two-way clustering analyses characterized the relationship between organisms and food sources. As a result of comparing metabarcoding of gut contents for P. argentata between sampled from Gwangyang Bay and the fisheries market, fish and Copepoda were analyzed as common food sources. In addition, Decapoda and Copepoda were analyzed as common food sources for L. polyactis and C. affinis, respectively. Copepoda was analyzed as the primary food source for L. beka and S. officinalis. These study results demonstrated that gut contents analysis using DNA metabarcoding reflects diverse and detailed information of biological food sources in the aquatic environment. In addition, it will be possible to provide biological information in the gut to identify key food sources by applying it to the research on the food web in the ecosystem.

Sampling gears for collecting fish are diverse, and the community of fish varies according to the selection and characteristics of the sampling gears. The present study compared the characteristics of fish communities in Yedang reservoir using four sampling gears (kick net, cast net, gill net, and fyke net). The kick net and cast net were inefficient in collecting the number of individuals. However, they increased the species diversity of fish inhabiting the waterfront. Although not many individuals were collected, the gill net mainly collected large fish. The largest number of individuals was collected in the fyke net, and the dominance was high due to the high species selectivity. Through Self-Organizing Map (SOM) analysis, large fish were collected in the gill net, whereas small fish were collected in the fyke net. The characteristics and efficiency of the fish differed depending on the sampling gears. It is expected that researchers will need to use it appropriately according to the characteristics of the sampling gears when investigating the fish community.

In this study, BSC model of center for teaching and learning was developed using balanced scorecard suitable for non-profit organization. Firstly, relevant literature surveys and evaluation indicators of various CTL and institution with similar characteristics were examined. Next, a draft BSC model was designed through interviews of specialists. Lastly, the BSC model was proposed by verifying the content validity of the evaluation model by conducting two Delphi surveys. The BSC model of CTL has 4 perspectives: resource, customer, internal process, learning and growth, 9 critical success factors: 2 factors in resource, customer and learning and growth perspectives, 3 factors in internal process perspective, and 23 key performance Indicators: 4 indicators in resource and learning and growth, 7 indicators in customer perspective, 8 indicators in internal process perspective. The implications of this study through the results were as follows: firstly, the proposed BSC model showed an evaluation model suitable for a non-profit organization. Second, the BSC model was linked to the organization’s mission and vision. Third, it could contribute to the long-term development of CTL. Lastly, if it could be applied to management, and evaluated, it is expected to play a role of providing basic data for the budget support and spread of the university.

Among the information technology and automation that have rapidly developed in the manufacturing industries recently, tens of thousands of quality variables are estimated and categorized in database every day. The former existing statistical methods, or variable selection and interpretation by experts, place limits on proper judgment. Accordingly, various data mining methods, including decision tree analysis, have been developed in recent years. Cart and C5.0 are representative algorithms for decision tree analysis, but these algorithms have limits in defining the tolerance of continuous explanatory variables. Also, target variables are restricted by the information that indicates only the quality of the products like the rate of defective products. Therefore it is essential to develop an algorithm that improves upon Cart and C5.0 and allows access to new quality information such as loss cost. In this study, a new algorithm was developed not only to find the major variables which minimize the target variable, loss cost, but also to overcome the limits of Cart and C5.0. The new algorithm is one that defines tolerance of variables systematically by adopting 3 categories of the continuous explanatory variables. The characteristics of larger-the-better was presumed in the environment of programming R to compare the performance among the new algorithm and existing ones, and 10 simulations were performed with 1,000 data sets for each variable. The performance of the new algorithm was verified through a mean test of loss cost. As a result of the verification show, the new algorithm found that the tolerance of continuous explanatory variables lowered loss cost more than existing ones in the larger is better characteristics. In a conclusion, the new algorithm could be used to find the tolerance of continuous explanatory variables to minimize the loss in the process taking into account the loss cost of the products.1)

본 연구에서는 버섯의 종류 및 추출 방법에 따른 성분함량의 차이를 확인위한 연구를 진행하였다. 선발한 버섯의 종류는 시중에서 판매중인 표고, 큰느타리, 양송이, 느타리, 팽이, 목이, 만가닥, 아위, 황금송이 등 9종류이다. 추출은 열수 추출 및 EtOH 추출을 진행하였다. 열수 추출물은 각 버섯 100g을 각각 열배수인 1L의 증류수로 3시간, 121°C에서 열수 추출하여 제조하였다. EtOH 추출물은 마찬가지로 100g의 버섯을 각 각 70% EtOH 1L로 24시간, 45°C에서 150rpm으로 진탕하여 제조하였다. 제조된 추출물은 HPLC 기기분석 을 통해 분석하였다. 분석항목은 1-deoxynojirimycin(DNJ), γ-amino butyric acid(GABA) 등 유리아미노산, 유 기산 등이다. 분석 결과 모든 버섯의 열수 추출물과 EtOH 추출물 전부 DNJ는 검출되지 않았다. 추출물의 유기산 농도는 EtOH 추출물보다 열수 추출물에서 월등히 높게 나타났으며, 특히 malic acid의 함량이 전체 적으로 가장 높게 측정되었다. 각 버섯의 열수 추출물 중 표고버섯의 총 유기산 함량이 14911.77 μg/mL로 가장 높았으며, 반대로 목이버섯의 총 유기산 함량은 556.17 μg/mL로 가장 적었다. EtOH 추출물에서는 glutamic acid를 제외한 유기산은 거의 측정되지 않았다.

In the manufacturing industry fields, thousands of quality characteristics are measured in a day because the systems of process have been automated through the development of computer and improvement of techniques. Also, the process has been monitored in database in real time. Particularly, the data in the design step of the process have contributed to the product that customers have required through getting useful information from the data and reflecting them to the design of product. In this study, first, characteristics and variables affecting to them in the data of the design step of the process were analyzed by decision tree to find out the relation between explanatory and target variables. Second, the tolerance of continuous variables influencing on the target variable primarily was shown by the application of algorithm of decision tree, C4.5. Finally, the target variable, loss, was calculated by a loss function of Taguchi and analyzed. In this paper, the general method that the value of continuous explanatory variables has been used intactly not to be transformed to the discrete value and new method that the value of continuous explanatory variables was divided into 3 categories were compared. As a result, first, the tolerance obtained from the new method was more effective in decreasing the target variable, loss, than general method. In addition, the tolerance levels for the continuous explanatory variables to be chosen of the major variables were calculated. In further research, a systematic method using decision tree of data mining needs to be developed in order to categorize continuous variables under various scenarios of loss function.

Many enterprises have been adopting management systems good enough for them and operating the schemes to have competitive edge better than their competitor. One of the finest systems that most companies have been selecting is ISO 14001. ISO 14001, environment management system, is widely known as advancing internal efficiency and productivity in the various industries. As a result, the number of companies that have adopted ISO 14001’s certification have been increasing continuously year by year since ISO 14000 standard was called in 1994s from ISO 18000 standard that originally used. The purpose of this study is to present that ISO 14001’s requirement execution influences on management performance in the small manufacturer. In order to carry out this research, we obtained 96 survey sheets and inspected ten assumptions based on the reliability, validity of the questionnaires. The results of regression analysis are summarized as follows; First, the three factors of ISO 14001 requirement, plan, implementation and operation and management consideration, have an important effect on the operational performance. Second, the three elements of ISO 14001 requirement, environment policy, implementation and operation and management consideration, affect to the financial performance significantly. Based on the results, it is sure that the companies that execute the factors of implementation and operation and management policy have a good performance to achieve operational and financial performance so that it is important to clarify the member’s role, authority and follow the procedure by a company’s process. Also, the truth shows CEO’s continuous will and periodical review of ISO 14001 influence on management performance as well.

ISO 9001, quality management system, is widely known as advancing internal efficiency and productivity in various industry. As a result, the number of companies that have adopted ISO 9001’s certification have been increasing continuously year by year since ISO 9000’ family standard was published in 1987s. This fact shows that a lot of businesses are trying to get the accreditation for improving the competitiveness of quality. The purpose of this study is to present that ISO 9001’s certification motives affect to the requirement’s implementation and show that the execution of requirements influences on management performance in the small manufacturer. In order to carry out this research, we obtained 96 survey sheets and inspected twenty assumptions based on the reliability, validity of the questionnaires. The results of regression analysis are summarized as follows; First, the factors of certification motive influence on the requirement’s implementation except resources management. Second, the two elements of ISO 9001 requirement, resources management and product realization, have an important effect on the operational performance and financial performance considerably in the companies that choose the internal motive. Finally, the two requisites of ISO 9001 requirement, resources management and measurement, analysis and improvement, affect to the operational performance and financial performance significantly in the businesses that select the external motive. Based on the results, the companies that choose the internal motive have a good performance to execute the ISO 9001 requirements. Also, to implement the requirements of ISO 9001 is helpful for the companies to produce the operational and financial performance.

The purpose of this study is to present that the entrepreneurial orientation affects to management performance in the small manufacturer. In order to carry out this research, we obtained 171 survey sheets and inspected seven assumptions based on the reliability, validity of the questionnaires. The results of regression analysis are summarized as follows; First, the factor of entrepreneurial orientation, innovative-ness, influences on the technical innovation performance and financial performance significantly. Second, the element of entrepreneurial orientation, proactive-ness, has an important effect on the technical innovation performance and financial performance. Finally, the requisite of entrepreneurial orientation, risk taking, affects to the technical innovation performance significantly, but not the financial performance. Based on the results, the companies that achieve the technical innovation and financial performance have an atmosphere to encourage to perform the research and development works and find out innovative ideas and improvement points in processes. However, the entrepreneur is required to take careful judgement for determining the uncertain circumstance.

The purpose of this study is to introduce a empirical study of using Taguchi Method for the Entrepreneurial Orientation and Financial Performance. In this study, three factors of Entrepreneurial Orientation, innovation, pro-activity and risk taking, are used of the control factors and an element, period of establishment, is chosen as a noise factor. In order to carry out this research, we obtained 200 survey sheets and performed the reliability and validity analysis of the questionnaires and then the experiment was conducted by Taguchi's experimental design. To improve Financial Performance under the noise factor of period of establishment, optimal condition are as follows; the two factors of Entrepreneurial Orientation, innovation, pro-activity should be maintained to a high level. Also, other factor, risk taking should be performed as the level that the company has maintained. Through this paper, we find out that Taguchi method is applicable for analysis of questionnaire.

Multivariate control charts are widely needed to monitor the production processes in various industry. Among the several multivariate control charts, control chart have been used of the typical technique. The control chart shows a statistic that represents observed variables and monitors the process through the statistic. In this case, the statistic generally have the limit that any variables affect to that statistic. To solve this problem, some studies have been progressed in the meantime. The representative method is to disassemble total statistic into each of the variable value and make a decision the parameters with large values than threshold value as a main cause. However, the means is requested to follow the normal distribution. To settle this problem, the bootstrap technique that don't be needed the probability distribution was introduced in 2011. In this paper, I introduced the detection technique of the fault variables using multiple regression analysis. There are two advantages; First, it is possible to use less samples than the ascertainment technique applying to bootstrap. Second, the technique using the regression analysis is easy to apply to the actual environment because the global threshold value is used.

This study was carried out to study the survival rate of thawed Hanwoo embryos frozen by the slow-rate freezing or the cryotop vitrification method. Hanwoo cumulus-oocyte complexes were recovered from ovaries at a slaughter house, matured for 20~22 hours, fertilized with Hanwoo semen for 5~6 hours, and cultured for 7~9 days in 38.5℃, 5% CO2 incubator. For freezing, Day 7∼9 blastocysts were collected. Embryos for the slow-rate freezing were equilibrated in 1.8 M ethylene glycol (EG) with Dulbecco's phosphate-buffered saline (D-PBS). Programmable cell freezer was precooled down to —7℃, and the straw was seeded during 8 minutes-holding time, and was cooled to —35℃ at the cooling rate of 0.3℃/min, and then was plunged and stored in liquid nitrogen. Embryos for the cryotop vitrification were treated in TCM199 with 0.5 M sucrose, 16% EG, 16% dimethylsulfoxide (DMSO). Embryos were then loaded individually onto cryotop and plunged directly into liquid nitrogen. The survival rates of embryos frozen by these two freezing methods were evaluated at 12 to 24h post-thawing. The survival rates of frozen/thawed Hanwoo embryos by the cryotop vitrification method (56.86 ± 26.53%) were slightly higher than those by the slow-rate freezing method (55.07 ± 26.43%) with no significant difference. Using the cryotop vitrification and the slow-rate freezing of Hanwoo blastocysts on Day 7 following in-vitro fertilization (IVF) treatment, the survival rates of frozen/thawed Hanwoo embryos were 72.65 ± 18.3% and 79.06 ± 17.8%, respectively. The survival rates by the cryotop vitrification were higher than those by the slow-rate freezing on both Day 8 and 9 with significantly higher survival rate on Day 9 (p<0.05). Using the cryotop vitrification and the slow-rate freezing of Hanwoo embryos to compare between three different blastocyst stages, the survival rates of the blastocyst stage embryos were 66.22 ± 18.8% and 45.76 ± 12.8%, respectively with higher survival rate by the vitrification method (p<0.05). And the survival rate of expanded blastocysts was higher than those of early blastocysts and blastocysts in two freezing methods with significantly higher survival rate by the slow-rate freezing method (p<0.05).

본 연구는 중국 소비자를 대상으로 명품 브랜드 확장 시 영향의 요인을 살펴보기 위해 모브랜드의 특성 및 확장제품 간의 관계를 통합적으로 고찰하였다. 첫째, 모브랜드의 특성으로는 브랜드의 명품성 정도에 따라 명품 브랜드와 비명품 브랜드를 선정하여 브랜드 명품성이 브랜드 확장에 미치는 영향을 살펴보았다. 실증 연구 결과 소비자들은 명품 브랜드의 확장제품에 대해 비명품 브랜드의 확장제품보다 브랜드 확장에 대해 긍정적으로 평가하였다. 둘째, 확장제품의 일치성이 브랜드 확장에 미치는 영향을 알아보고자 하였다. 실증연구 결과 소비자들은 일치, 극도로 불일치한 확장제품보다 적당히 불일치한 확장제품을 더 긍정적으로 평가하였다. 또한 명품 브랜드, 비명품 브랜드에 적용하여 본 결과에서도 적당히 불일치한 브랜드 확장에 더 높은 호감도를 보였다. 마지막으로 브랜드의 명품성과 확장제품 일치성의 상호작용에 따른 브랜드 확장 평가에 미치는 영향에 대해 살펴보았는데 중국소비자들을 대상으로 실증연구한 결과 브랜드의 명품성과 확장제품의 일치성 정도는 브랜드 확장 평가에 상호작용을 나타내었다. 이러한 결과는 중국 소비자들에게 어필하기 위해서는 ‘브랜드의 명품성’과 ‘확장제품의 일치성’이라는 변인을 접목시켜 브랜드 확장 전략을 사용해야 됨을 실증한 결과이다. 따라서 두 변인 간의 연결 고리를 확고히 하고, 브랜드 확장을 통해 중국 소비자들과의 긴밀한 관계를 형성하는 것이 효과적으로 자사의 브랜드 확장 제품을 인식시킬 수 있다는 시사점을 제공해 준다.

RC shear wall sections which have irregular shapes such as T, ㄱ, ㄷ sections are typically used in low-rise buildings in Korea. Pushover analysis of building containing such members costs a lot of computation time and needs professional knowledge since it requires complicated modeling and, sometimes, fails to converge. In this study, a method using an equivalent column element for the shear wall is proposed. The equivalent column element consists of an elastic column, an inelastic rotational spring, and rigid beams. The inelastic properties of the rotational spring represent the nonlinear behavior of the shearwall and are obtained from the section analysis results and moment distribution for the member. The use of an axial force to compensate the difference in the axial deformation between the equivalent column element and the actual shear wall is also proposed. The proposed method is applied for the pushover analysis of a 5- story shear wall-frame building and the results are compared with ones using the fiber elements. The comparison shows that the inelastic behavior at the same drift was comparable. However, the performance points estimated using the pushover curves showed some deviations, which seem to be caused by the differences of estimated yield point and damping ratios.

본 연구는 244,443 도체로부터 얻은 도체특성(성별, 등지방두께 및 도체중), 사육환경(자돈의 성별 분리사육, 돈사의 온도관리 유무, 돈사의 형태, 환기방식, 급수원의 종류, 음수 개선시설의 유무, 돈사의 연간 소독횟수, 돈사의 바닥형태 및 평당 사육두수), 출하방법(선별출하, 출하일령, 등지방두께의 편차 및 도체중의 편차) 및 번식특성과 자돈관리(모돈의 산차, 이유일령, 정액의 분양처 및 자돈의 품종)등의 자료를 바탕으로 돼지의 도체가격에 미치는 영향을 분석하기 위해 실시하였다. 도체특성 변수에서 암퇘지 및 거세돈이 숫퇘지보다 높은 가격을 형성하였으며(p<0.05), 등지방두께 15-29mm 및 도체중 91-95kg가 높은 도체가격을 보였다(p<0.05). 도체가격에 대한 등지방 두께 및 도체중은 도체가격 형성에 각각 90.75%와 90.23%를 차지하였다. 하지만, 사육환경, 출하방법, 번식관리는 각각의 방법별로 가격에 유의적인 차이를 보였으나, 도체가격형성에는 낮은 영향을 미쳤다. 이상의 결과 여러 가지 변수 중 등지방 두께 및 도체중이 도체가격에 큰 영향을 미친 것으로 나타났다.

This study was performed to investigate the possibility of repeated superovulation treatment at interval from 27 days to 41 days in Hanwoo cattle and to compare with superovulation effect between doses of FSH 200 mg and FSH 400 mg. Different doses of FSH (200 mg or 400 mg) were injected at Day 8 after controlled internal drug release (CIDR) treatment for superovulation of Hanwoo donors following CIDR treatment (Day 8 after the estrus). Superovulation was repeated four times for one donor and number of corpus luteum (CL), number of embryos, number of transferable embryos and pregnancy rate after embryo transfer (ET) were investigated. 5 cows were used for each FSH treatment (10 cows in total). Average number of CL were and for the donors treated with FSH 200mg and FSH 400mg, respectively. Average number of embryos collected were and for the donors treated with FSH 200 mg and FSH 400 mg, respectively. Average number of transferable embryos were and for the donors treated with FSH 200 mg and FSH 400 mg, respectively. The pregnancy rate following ET with embryos collected from 200 mg FSH treated donors and 400 mg FSH treated donors were 61.9% and 53.8% respectively. The numbers of embryos tended to be decreased as the numbers of repeat of superovulation were elapsed. These results indicated that superovulation treatment by about a month to Hanwoo donors is usable and 200 mg of FSH is preferable for simple FSH treatment following CIDR treatment.