This study investigated the fish fauna of Jeonjucheon and Samcheon Streams, flowing through the city of Jeonju, through surveys conducted at 10 sampling sites using kick net and cast net. The fish fauna collected included 10 families and 36 species, with a total of 2,064 individuals. Samcheon had 8 families and 30 species with 1,074 individuals, Jeonjucheon had 8 families and 26 species with 986 individuals. The dominant species was Zacco platypus with 1,202 individuals, and a total of 153 individuals of the subdominant were Pseudogobio esocinus. In Jeonjucheon Stream, a total of 567 individuals of the dominant species were collected as Z. platypus and 99 individuals of the subdominant species were collected as Pungtungia herzi, and there was no significant change in the stream environment except for the confluence site. Further expanded research covering the entire Jeonjucheon and Samcheon Streams region, accompanied by regular monitoring, is essential to record and understand fluctuations in fish fauna.