Metabolomics aims at the comprehensive, qualitative and quantitative analysis of wide arrays of endogenous metabolites in biological samples. It has shown particular promise in the area of toxicology and drug development, functional genomics, system biology and clinical diagnosis. In this study, analytical technique of MS instrument with high resolution mass measurement, such as time-of-flight (TOF) was validated for the purpose of investigation of amino acids, sugars and fatty acids. Rat urine and serum samples were extracted by selected each solvent (50% acetonitrile, 100% acetonitrile, acetone, methanol, water, ether) extraction method. We determined the optimized liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) system and selected appropriated columns, mobile phases, fragment energy and collision energy, which could search 17 metabolites. The spectral data collected from LC/TOFMS were tested by ANOVA. Obtained with the use of LC/TOFMS technique, our results indicated that (1) MS and MS/MS parameters were optimized and most abundant product ion of each metabolite were selected to be monitorized; (2) with design of experiment analysis, methanol yielded the optimal extraction efficiency. Therefore, the results of this study are expected to be useful in the endogenous metabolite fields according to validated SOP for endogenous amino acids, sugars and fatty acids.

본 연구는 천연 인듐의 중성자폭획 후에 발생된 감마선을 12개의 BGO(Bi4Ge3O12)섬광검출기로 구성된 검출장치를 이용하여 즉발감마선을 측정하여 중성자공명에의해서 발생된 감마선을 분석하여 중성자의 에너지 1 ~ 300 eV영역에 대하여 공명에너지를 분석하였다. 사용되어진 검출장치는 시료에서 발생된 즉발감마선에 대하여 모든 감마선을 측정할 수 있는 기하학적인 구조로 만들어졌다. 중성자원으로는 교토대학의 원자로연구소의 46-MeV 전자선형가속기의 광핵반응에서 발생하는 중성자를 이용하였다. 중성자발생원으로부터 검출기까지의 거리가 12.7 ± 0.02m 이므로 광핵반응에서 발생하는 강한 X선의 영향을 고려하여 수 keV영역이하의 중성자에너지에 대하여 중성자공명을 측정하였다. 측정되어진 공명들은 1 eV이상의 에너지 영역에서 거대 공명들을 측정하였고 이들 공명들은 이론 에서 알려진 값들과 비교하였다. 본 연구를 통하여 기존에 알려진 거대 공명의 에너지를 확인하였고, 100 eV이상의에너지 영역에서의 공명에너지들에 대한 평가에 의한 이론값들이 실제로 존재하는 공명임을 실험적으로 확인하였다. 1 keV이상의 영역에 대하여는 공명이 연속적인 구조를 보이고 있음을 실험적으로 확인했으며 공명에 대하여 통계적인 평가가 있어야함을 알았다. 91.49 eV 공명은 본 연구를 통하여 처음 발견되어진 공명이라 볼 수 있다.

The neutron capture spectrum for the light nuclide was very useful to study the nuclear structure. In the present study, the capture gamma-ray from the 27-keV resonance of 19F(n,g)20F reaction were measured with an anti-Compton NaI(Tl) spectrometer and the 3-MV Pelletron accelerator of the Research Laboratory for Nuclear Reactors at the Tokyo institute of technology. A neutron Time-of-Flight method was adopted with a 1.5 ns pulsed neutron source by the 7Li(p,n)7Be reaction. In the present experiment, a Teflon((CF2)n) sample was used The sample was disk with a diameter of 90mm. The thickness of sample was determined so that reasonable counting rates could be obtained and the correction was not so large for the self-shielding and multiple scattering of neutrons in the sample, and was 5mm. The primary gamma-ray transitions were compared with previous measurement of Kenny.