Total phenolic compounds and antioxidant activity were investigated in leaves of organic apricot, filbert, mulberry, persimmon, and pomegranate trees during growing season. Total phenolic compounds and antioxidant activity in leaves of organic apricot and filbert trees were gradually increased from May to June, attained peak in July, thereafter decreased to minimum level in October. In leaves of organic mulberry tree total phenolic compounds and antioxidant activity were simultaneously increased from May to June and sharply decrease to very low level. The leaves of organic persimmon trees showed very high level of total phenolic compounds and antioxidant activity from May to June, thereafter gradually decrease to October. The leaves of organic pomegranate trees showed extraordinarily highest level of total phenolic compounds among five fruit trees investigated in the study during growing stages, as well as maintained higher than 91 percent of antioxidant activity from May to October. Correlation coefficients between total phenolic compound and antioxidant activity of the leaves of five organic trees were the highest in mulberry leaves, and then persimmon, filbert, apricot, and pomegranate, respectively. However, correlation coefficients between total phenolic compound and antioxidant activity of the leaves of organic pomegranate were very low level, and not significant in their relationship.
본 연구는 우리나라에서 보존하고 있는 구기자(Lycium chinesis Miller) 유전자원 131점의 잎과 줄기를 에탄올로 추출한 후 페놀화합물의 함량을 측정하여 비교 분석하기 위하여 수행되었다. 구기자 잎의 페놀 함량은 8.8~14.9mgg1을 보였으며 11.6~13.5mgg1 사이에는 약 60%의 유전자원이 분포되어 있었다. CB03286-89 유전자원은 총 페놀함량이 14.9±0.3mgg1으로 가장 높은 반면 CBP03310-250은 8.8±0.2mgg1으로 가장 낮은 함량을 보였다. 줄기에 대한 총 페놀함량은 6.8~12.4mgg1의 함량 범위를 보이고 있으며 잎보다는 상대적으로 다소 낮은 것으로 나타났다. 구기자 잎의 페놀화합물 중 33가지 물질을 정량 분석한 결과 China collection No.1에서 가장 높게 나타났으며 99797 수집종에서 가장 낮게 나타났다. (+)catechin 함량은 CB03286-89에서 가장 많았다. Myricetin은 Geumsan jaerae, Japan No.1, China collection No.1, CL32-13, CB04329-13, China collection No.12 그리고 CB03286-89 등에서 분석되었지만 나머지 구기자 유전자원의 잎에서는 검출되지 않았다. 줄기에 대한 페놀화합물은 잎에 비하여 상대적으로 함량이 적었으며 99797 수집종이 가장 많은 함량을 보였으나 CB04329-13에서는 가장 적은 함량을 보였다. (+)catechin 함량은 Japan No.1이 231.0±0.5ugg1으로 가장 높았으며, myricetin은 Japan No.1, China collection No.1, China collection No.12, CB03286-89 및 99797에서 분석되었지만 나머지 구기자 유전자원의 줄기에서는 검출되지 않았다.
Artichoke extracts are widely used alone or in association with other herbs for embittering alcoholic and soft drinks and to prepare herbal teas or herbal medicinal products in Viet Nam. The objective of this paper was a screening of flavonoids and total phenolic compounds content in the parts of artichoke (Cynara scolymus L.) as flowers, leaves, roots, trunks, stumps. The total phenolic compounds and flavonoids in the parts of artichoke were extracted among 3 extraction methods as methanol extraction (EM1), mixing methanol and water method (EM2) and water extraction method (EM3). Total phenolic compounds and flavonoids were determined by UV/VIS, HPLC techniques. The apigenin 7-O-glucosides, cynarin, narirutin, gallic acid, caffeic acid were found as the main flavonoids constituents in all parts of artichoke. It showed that value of total phenolic compounds and flavonoids by EM3 were higher than that of total phenolic compounds and flavonoids by EM1 and EM2. Furthermore, the results of this study revealed that total phenolic compounds and flavonoids, obtained by these convenient extraction methods, may show the quick efficacy of artichoke in all respects of their quality and quantity.
This objective of this study was to determined the activity of antioxidants of DPPH, SOD and total phenolic compounds in mushroom(P. eryngii spp.) extract. The antioxidant acitivity using DPPH showed that strain No. 2007(31.78~%) and strain No.2014(27.45~%) were the highest DPPH inhibition. Among the thirteen mushroom examined, strain No. 2014(58.30~%) and strain No. 2010(57.19~%) had the highest SOD activity. The highest total phenol content were in strain No.2013(1465.03~mug/g) and strain No.2005(1401.09~mug/g) . Our study suggest that it may be possible to improve mushroom with high natural antioxidant.
Cereal grains are rich in phenolic compounds that give beneficial effect in human health. Although several research works have been reported on the effects of phytochemicals of plant origin, such as fruits, vegetables, few studies have examined the antioxidative effects of whole cereal grains. The objective of this study was to determine total antioxidant capacity of 80% ethanolic extracts of cereal grains by testing the ability of the extracts to inhibit UV -induced lipid peroxidation in vitro using linoleic acid in comparison to well-known antioxidant such as ascorbic acid and tannic acid. The total phenolic content of the cereal grain (80% ethanolic extracts) investigated in this study varied from 2.1 mg/g (wheat cv. Olgeurumil) to 10.4 mg/g (barely cv. Seodunchalbori). Highly positive relationship between total phenol compounds and antioxidant activity was found. When the antioxidant activities of all investigated extracts were measured with application of same quantity of phenol compounds, oat grain extracts showed similar antioxidant activity of barely cultivars. However, barely extract appeared as the most potent antioxidant activity of inhibition of UV -induced lipid peroxidation. This indicated that factors such as phenolic compound composition and their individual antioxidant activity could playa crucial role in the total antioxidant activity of cereal grains.