Characteristics related to grain quality and starch viscosity were investigated in three independent japonica transgenicrice lines expressing a Protox gene from Bacillus subtilis and compared to the control. In this study, we found that there were nosigni

Transgenic rice plants expressing a Bacillus subtilis protoporphyrinogen oxidase (Protox), the last shared enzyme of the porphyrin pathway in the expressed cytoplasm or the plastids, were compared with non-trangenic rice plants in their growth characteristics such as tiller number, plant height, biomass, and yield. Transgenic rice plants of ~textrmT3 generation had 8 to 15 % and 25 to 43% increases in tiller number compared to non-transgenic rice plants at 4 and 8 weeks after transplanting(WAT); similar values were observed for ~textrmT4 generation at 4 and 8 WAT. However, the plant height in both ~textrmT3 and ~textrmT4 generations was similar between transgenic rice plants and non-transgenic rice plants at 4 and 8 WAT. Transgenic rice plants had 13 to 32% increase in above-ground biomass and 9 to 28% increase in grain yield compared to non-transgenic rice plants, demonstrating that biomass and yield correlate with each other. The increased grain yield of the transgenic rice plants was closely associated with the increased panicle number per plant. The percent of filled grain, thousand grains and spikelet number per panicle were similar between transgenic and non-transgenic rice plants. Generally, the growth and yield of transgenic generations (~textrmT2 , ~textrmT3 , and ~textrmT4 ) and gene expressing sites (cytoplasm-expressed and plastid-targeted transgenic rice plants) were similar, although they slightly varied with generations as well as with gene expressing sites. The transgenic rice plants had promotive effects, indicating that regulation of the porphyrin pathway by expression of B. subtilis Protox in rice influences plant growth and yield.

Bacillus subtilis PANH765 균주가 생산하는 pretense를 황산암모늄에 의한 염석, DEAE-cellulose ion exchange chromatography, Sephacryl S 200 HR 및 Sepharose CL-6B gel filtration을 이용하여 정제하였다. DEAE-cellulose ion exchange chromatography를 행한 결과, 흡착 단백질 부분에서 활성이 높은 분획을 얻었다. 이 분획

단백질분해효소를 생산하지 않는 균주 B. subtilis MT-2의 염색체 DNA를 추출한 다음, B. subtilis AC819 균주에 상동성 유전자재조합을 이용하여 competent cell 형질전환을 시켰다. 얻어진 형질전환체를 B. subtilis HL-1이라고 명명하였으며, 그 표현형은 histidine 요구성, streptomycin 내성, tetracyclin 내성을 나타내면서 단백질 분해효소를 생산하지 않았다. 플라스미드 pUB11