본 연구에서는 참외과실의 수확후 식용왁스코팅처리가 과실품질 및 과골 갈변에 미치는 대사체의 변화를 분석하였다. 코코넛 오일과 밀랍 (9:1 w/w) 처리를 참외 과실표면에 코팅 후 실온 저장 7일후에 과실 품질, 에틸렌 발생량 및 호흡량을 조사하였다. 그 결과, 참외의 경도와 골갈변도는 식용코팅처리된 과실과 무처리군 사이에 유의한 차이는 없었다. 하지만, 과피색의 명도(L*), 순도(a*), 및 색상각(hue angle)은 식용왁스처리군이 무처리군에 비해 유의한 차이가 있었다. 이는 호흡량 및 에틸렌 발생량이 식용왁스처리에 의해 유의하게 낮아져 과피색이 유지된 것으로 생각된다. 식용왁스 처리에 의한 참외의 대사체 변화를 분석한 결과, gamma-aminobutyric acid, aspartate 그리고 myo-inositol 대사가 가장 유의적인 차이를 나타났으며, 특히 삼투보호제 역할을 하는 alanine, 가바(GABA)성분이 식용왁스코팅에 의해 증가하였다. 따라서, 식용왁스코팅처리는 호흡과 에틸렌 발생의 억제, 수분 스트레스 경감 등을 통해 참외의 선도 유지를 유도한 것으로 판단되며, 참외 과피색 변화 억제 등 상품성 향상에 활용될 수 있을 것으로 보인다.

Liquid crystal (LC) system was introduced into W/O emulsion in order to enhance the stability and moisturizing effect. The LC system, composed of beeswax, surfactant, and water was formed on the surface of emulsion droplet, which was investigated through a polarized microscope. The phenomenon that the viscosity in W/O emulsion system is decreased with time, was reduced by the formation of LC with the addition of beeswax. Centrifugal separation test showed that the stability of emulsion system was increased with the addition of beeswax to 3%. The color change of vitamin C was delayed in LC emulsion systems, which indicates stabilization effect against the oxidation of vitamin C. Evaporation rate in W/O emulsion was retarded by LC, so that high moisturizing effect is expected in W/O LC system.

The beeswax sample was collected from the beehives, isolated and then refined. The first step of producing beeswax was to separate honey from beehives. The beehives which were cut put in hot water. The upper layer was crude beeswax, which was treated with phosphoric acid. The crude beeswax was purified through the bleaching. The objectives of this study are to identify headspace volatile compounds and to know the contents of α-carotenes and β-carotenes of korean beeswax. Headspace volatile compounds of Korean beeswax were measured by using the combination of dynamic headspace sampler (DS 5000, Donam System Inc.), gas chromatography and mass selective detector (HP5890 & 5971, Hewlett Packard). Seventy five compounds identified from about 100 peaks by analyzing the purified beeswax were 60 hydrocarbons, 8 carbonyls, 4 essential oils, 3 esters. Carotenes of Korean beeswax were analyzed by using High Performance Liquid Chromatography (Waters Inc.). As A result, the content of α-carotenes and β-carotenes were 0.07ppm, 0.011ppm individually.