This study was conducted to evaluate the applicability of a carrier media with natural minerals as packing material in a biofilter to remove odor-causing compounds. The carriers were prepared by mixing powdered zeolite, barley stone, and clay. They have a pellet type with a length of 5m m to 10 mm, 3.2 m2/g of a specific surface area, and 0.04 cm3/g of a pore volume. The adsorption capacity and the biodegradation by biomass formation on the media were experimented with toluene and ammonia as the test compounds. The carrier possessed the ability to adsorb toluene and ammonia. The adsorption capacity of toluene and ammonia at the inlet concentration of 100 ppmv was 58 g/g and 96 g/g, respectively. In the biofilter using the carrier as the packing material, the biofilter performances were different depending on the supply of moisture and liquid-nutrient. The critical loading was found to be 33.13 g/m3/hr for toluene removal and 6.5g /m3/hr for ammonia removal even when no nutrients were supplied. The proposed material has been confirmed to be capable of adsorbing inorganic and organic compounds, and can be effectively applied as packing materials for the biofiltration.

Nitrate contamination of water environments can create serious problems such as eutrophication of rivers. Conventional biological processes for nitrate removal by heterotrophic denitrification often need additional organic substrates as carbon sources and electron donors. We tried to accelerate biological denitrification by using bioelectrochemical reactor (BER) in which electrode works as an electron donor. Denitrification activity of 8 environmental samples from various sediments, soils, groundwaters, and sludges were tested to establish an efficient enrichment culture for BER. The established enrichment culture from a soil sample showed stable denitrification activity without any nitrite accumulation. Microbial community analysis by using PCR-DGGE method revealed that dominant denitrifiers in the enrichment culture were Pantoea sp., Cronobacter sakazakii, and Castellaniella defragrans. Denitrification rate (0.08 kg/m3·day) of the enrichment culture in BER with electrode poised at -0.5 V (vs Ag/AgCl) was higher than that (2.1×10-2kg/m3·day) of BER without any poised potential. This results suggested that biological denitrification would be improved by supplying potential throughout electrode in BER. Further research using BER without any organic substrate addition is needed to apply this system for bioremediation of water and wastewater contaminated by nitrate.

This study was conducted to determine optimum design parameters in nitrification and denitrfication of chemical fertilizer wastewater using pilot plant, Jet Loop Reactor. The chemical fertilizer wastewater which contains low amounts of organic carbon and has a high nitrogen concentration requires a post-denitrfication system. Organic nitrogen is hydrolyzed above 86%, and the concentration of organic nitrogen was influent wastewater 126㎎/L and of effluent wastewater 16.4㎎/L, respectively. The nitrification above 90% was acquired to TKN volumetric loading below 0.5 ㎏TKN/㎥‧d, TKN sludge loading below 0.1 ㎏TKN/㎏VSS‧d and SRT over 8days. The nitrification efficiency was 90% or more and the maximum specific nitrification rate was 184.8 ㎎TKN/L‧hr. The denitrification rate was above 95% and the concentration of NO3-N was below 20㎎/L. This case was required to 3 ㎏CH3OH/㎏NO3-N, and the effluent concentration of NO3--N was below 20㎎/L at NO3--N volumetric loading below 0.7 ㎏NO3--N/㎥‧d and NO3--N sludge loading below 0.12 ㎏NO3--N/㎏VSS‧d. At this case, the maximum sludge production was 0.83 ㎏TS/㎏T-Nre and the specific denitrfication rate was 5.5 ㎎NO3-N/ gVSS‧h.

The combined wastewater of municipal landfill leachate and municipal sewage was treated using several sets of bench-scale aerated circulating system including porous media. Investigated items in this experiment were the dominant protozoa and metazoa in this system, the variation of microfauna relationship between operating condition and dominant genera. Also considered the factors determining dominant genera and their role. The outcome of this research is as follows; 1. Aspidisca, Vorticella, Trachellophyllum, Lecane, Philodina, Cyclops were mainly appeared prior to combinding leachate, while Trachelocerca, Bodo, Glaucoma were the dominant genera after combinding leachate. 2. As to metazoa, Nematode and Philodina were not influenced by 5% leachate mixing ratio, meanwhile Crustacea has high sensitivity for increased leachate mixing ratio and it was not appeared in 5% leachate mixing ratio. 3. The appropriate treatability could`nt be expected at the above 10% leachate mixing ratio. Especially, in the condition of 20% leachate mixing ratio, all of the microfauna were affected damage seriously on their existence. Meanwhile hydraulic retention time, substrate loading rate and sludge production rate didn`t give notable influence on increasing the number of microfauna. 4. As to protozoa, saprozoic and holozoic species were appeared commonly and polysaprobic species were dominent. 5. Filamentous organsms were nearly not affeced by leachate mixing. It seems that they could live without any trouble at the 10% leachate mixing ratio, if the substrate is sufficient. 6. Diversity of microfauna had a reducing trernd as the sewage was mixed with leachate.