조직센서는 극 미량의 Na+채널 차단 물질을 측정하기 위해 개발된 고감도 측정 장치로 해조류 내 Na+ 채널 차단 생리활성물질의 양을 측정하기에 적합하였다. 본 연구는 해조류 내 Na+ 채널 차단 생리활성물질의 존재를 학인 함으로써 해조류를 이용한 신성분 개발의 기초자료를 확보할 목적으로 수행되었다. 분석에 사용된 해조류는 총 92종으로 녹조류 13종, 갈조류 42종, 홍조류 37종으로, 주요 해조류 내 Na+ 채널 차단 생리활성물질은

The acetaminophen (APAP), an antipyretic and analgesic agent, induces the hepatotoxicity by increasing influx of calcium and destabilizing the cellular membrane which can be caused by N-acetyl p-benzoquinoneimine generated by cytochrome P-450 (CYP-450) when it is overdosed. Diltiazem (DIL), a calcium channel blocking agent, has been known to suppress the CYP-450 activities. To study the effect of DIL in APAP treated rats, the serum biotransformational enzyme analyses and the liver histopathologic examination were conducted on the rats which had been administered DIL at 3, 6, 9 and 12 hours after the 3,000 mg/kg of APAP administration. Following a single dose of DIL administered 12 hours after APAP administration, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, malondialdehyde and calcium contents of liver and microsome were significantly reduced. Glutathione S-transferase (GST) activity was significantly increased. Histopathologic studies showed that DIL had prevented the development of centrilobular necrosis induced by APAP in liver tissue. Our results suggested that diltiazem could inhibit the formation of free radical and the influx of calcium and could increase GST activity. Therefore, diltiazem can be administered at the time of 12 hours after overdosed APAP to diminish the liver damage.