The purpose of this study was to investigate the protective effect on oxidative stress induced PC12 cells, and volatile flavor composition of essential oils derived from medicinal plant seeds- Gossypium hirsutum L. (G. hirsutum), Coix lachryma-jobi (C. lachryma-jobi) and Oenothera biennis (O. biennis). The essential oils were obtained by the solvent (hexane) extraction method from the seeds. The essential oils of the seeds were analyzed by the solid-phase micro-extraction gas chromatography mass spectrometry (SPME-GC/MS). The major compounds of G. hirsutum, C. lachryma-jobi and O. biennis were cyclonexanol (16.65%), β-asarone (14.29%) and ylangene (50.01%). The DPPH radical scavenging activity (IC50) was the highest value of 8.52 mg/mL in the O. biennis. Additionally, IC50 values of G. hirsutum and C. lachryma-jobi were 26.76 mg/mL and 36.81 mg/mL. For the oxidative stress on PC12 cells, we treated with hydrogen peroxide (H2O2). The pretreatment of oxidative stress induced PC12 cells with all the essential oils preserved or increased their cell viability and G. hirsutum and O. biennis attenuated the ROS generation (by 68.75% and 56.25% vs. H2O2 control). The results of this study suggest that the essential oils derived from medicinal plant seeds could be used as valuable back data as a natural essential oil material to prevent neurodegenerative diseases by protecting neuro-cells.

오이묘가 저온피해를 입기 전, 후 토양수분조건과 몇 가지의 화학물질처리가 오이묘의 냉해 경감에 미치는 영향을 연구한 결과 토양수분 -0.3bar 적습상태에서 07:00시에 2℃로 저온처리를 했을 때, 생존율은 28.3%이었으나 -5.5bar에서는 83.3%로 높아져 토양수분이 건조하면 저온피해가 감소되었다. 그러나 18:00시에 저온처리하면 -0.3bar에서도 생존율은 87% 이상이었다. 토양수분이 건조하면 엽신의 ABA함량은 크게 증가되었다. 저온처리 전, 후 ABA의 토양 및 엽면처리는 오이묘의 생존율을 유의하게 증가시켰고 세포의 전해질 누출량도 현저하게 감소시켰으며, 수량의 감소를 경감시켰다. 또한 ABA 처리는 저온처리 후보다는 저온처리 전에, 엽면처리보다는 토양에 처리하는 것이 보다 효과적이었다. ABA(10-5M)와 더불어 요소(0.2%)와 KH2PO4의 엽면살포는 오이의 생장과 수량에 미치는 저온피해를 경감시키는데 효과적이었다.

Sesame (Sesamum indicum L.) seeds contain abundant oil and antioxidative lignans related to the seed quality. To evaluate the potential effects of drought stress on the chemical composition of sesame seeds, eighteen cultivars were imposed water-deficit condition by withholding irrigation during 15 days at podding and maturing stage, compared with well-watered plants as control in seed yield and chemical composition. Drought treatments showed great decrease of seed yield with not affecting seed weight. The contents of sesamin and sesamolin decreased while lignan glycosides inversely increased in response to drought stress. Oil content was not significantly changed by drought treatment in spite of its slight decrease. In case of fatty acid composition, there were significant differences in increase of oleic acid while inverse decrease of linoleic acid under drought stress condition. These results demonstrate that the chemical composition of sesame seed may be modified with drought stress. In particular, the increase of sesaminol glucosides with strong antioxidative activity was observed.