Low molecular weight hydrolysates from donkey bone extracts (LHDB) was prepared with different food enzymes, and its antioxidative, elastase and collagenase inhibitory, and fibroblast cell protection effects against photoaging were evaluated. Gelatin from donkey bone was extracted three times at 121℃ for 1 h and was lyophilized. The lyophilized powder (5 g) was dissolved in 95 mL distilled water with 1% FoodPro alkaline protease (A), 1% Protease P (P), 1% Protease M (M) and a 0.3% A + 0.3% P + 0.3% M (APM) mixture and was hydrolyzed for 3 h at 45℃. After enzyme inactivation at 90℃ for 10 min, the LHDBs hydrolyzed by A, P, M, and APM were separated by centrifugal filtration and were lyophilized and marked as LHDB-A, LHDB-P, LHDB-M, LHDB-APM. The LHDB-M showed higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline–6- sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) than the other treatments (p<0.05). The elastase inhibition effect (37.49%) of LHDB-M were significantly higher than those of the other treatments (9.97-34.18%). The viability of human fibroblast cells (Hs68) after UVB irradiation was significantly increased by LHDB-M, indicating that it can be used as an antioxidant or as a UVB stress protector. However, further in vivo studies should precede its usage in the bioactive compound industry.

The present study was performed to determine beneficial effects of donkey meat extract supplementation (15 and 30 mg/kg B.W.) on streptozotocin (STZ) induced diabetic rat. Rump muscle of donkey was extracted by boiling in water for 3 hrs and supplemented to STZ induced diabetic rats for 6 weeks. Feed and water intake of STZ induced diabetic rats were higher than those of normal rats, while no significant difference was found in DME treated rats as compared to the rats in control group. STZ-induced diabetic rats did not improved their body weight as compared to that of rats in the control group. However, blood glucose level of 30 mg DME supplemented rats was found to be significantly reduced after 4 weeks of supplementation (p<0.05) when compared to the control animals. Administration of DME did not show any improving effect on serum lipids (HDL-cholesterol, total cholesterol, and triglyceride). No significant changes were noticed in terms of weight of all the visceral organs of animals fed on DME except liver. It is therefore, concluded that supplementation of DME with dose of 30 mg/B.W. resulted in decreased blood glucose levels, however, blood lipid profile of diabetic rats, having more than 400 mg/dl glucose level, did not change.