The relationship of several candidate genes polymorphisms with breeding values of economic traits were investigated in Korean Native Pigs. Record (2001-2006) of 546 Korean native pigs were obtained from National Institute of Animal Science, Korea having data on average daily gain (ADG), age at 70 kg (D70 kg) and backfat thickness (BF). The data's obtained were analyzed by the DF-REML (Derivative-Free Restricted Maximum Likelihood) program of Boldman using a single-trait animal model to analyse the genetic parameters. The analysis of restriction fragment length polymorphism (RFLP) was conducted on 68 Korean native pigs (KNP) using single nucleotide polymorphism (SNP). Different genotype frequencies of 5 candidate genes such as MC4R, PRKAG3, FABP3, ESR and PRLR3 were observed in KNP. Significant relationship of AA and AB genotype between MC4R polymorphic site and breeding value for average daily gain (ADG, p<0.05) was observed. PRKAG3 polymorphic sites were also found to be significantly related to breeding values for ADG, AA, AB genotype (p<0.05) and also, for Backfat thickness (BF), days to 70 kg and BB genotype (p<0.05). In conclusion, selection method would be more effective if it encompasses significant genotype for performance traits and that would further aid in the selection of seed stock in KNP.
There are four (yellow, brindle, black, black in Jeju) kinds of native cattle in Korea. But only twelve hundred Korean brindle cattle (KBC) are remaining in limited areas of Korea and the genetic lineage, diversity, polymorphisms of KBC has not been identified. To analysis genetic polymorphism of KBC, 33 KBC were characterized using 11 microsatellite markers. Size of microsatellite marker was decided using Gene Mapper software after analysis ABI 3130XL. The average of allele numbers of KBC was 6.7 in this study, but that of Hanwoo was 10.0 in our previous report. The observed and expected heterozygosities of KBC were 0.719 and 0.738 but those of Hanwoo were 0.751 and 0.760 in our previous report. Also polymorphism information content (PIC) values were average 0.690 in KBC but 0.725 in Hanwoo. These results demonstrate that genetic polymorphism of KBC have decreased because the population was limited.
The objective of this study was to evaluate the effects of co-culture of bovine oocytes with cumulus cells on in vitro maturation and development following in vitro fertilization in bovine oocytes. Bovine cumulus-oocyte complexes (COCs) and denuded oocytes (DO) were co-cultured with the cumulus cells in TCM199 for 20~22 hr, and evaluated the nuclear type of oocyte. After in vitro maturation, oocytes were coincubated for in vitro fertilization with frozen-thawed spermatozoa selected by 65% percoll in DM-Heparin and DM-Caffeine for 15~18 hr. Presumptive zygotes were cultured for 48 hr in CR1aa in vitro culture medium with 10% FBS, and evaluated the cleavage rates. The results confirmed that the highest percentage of metaphase II (M-II) stage was observed in COCs (30.1±3.5%, 24.2±1.8%) as compared to DO (7.1±1.3%, 17.4±13.9%) (p<0.05). In addition, the increased cleavage rates were obtained from COCs (69.6±2.1%, 75.6±2.9%) when compared to DO (21.6±7.5%, 29.5±12.6%) (p<0.05). In conclusion, this study suggested that cumulus cells secreted positive factors during in vitro maturation of oocytes and early embryonic development after in vitro fertilization of bovine oocytes.
This experiment was conducted to investigate the reasonable levels of energy and protein for the production of large broilers. A total of nine hundred sixty, day old broiler chickens (Ross) were randomly divided into six groups with four replicates of forty birds each. Experiments were factorial designed with 3,100, 3,000, and 3,030 kcal of ME/kg and 22, 21, and 21% CP for the first week; and 3,100, 3,035, and 3,050 kcal of ME/kg and 19, 19, and 18.5% CP for the second and third week; and 3,100, 3,175, and 3,100 kcal of ME/kg and 17, 18, and 17.5 CP for the fourth and fifth week; and 3,100, 3,175, and 3,250 kcal of ME/kg and 17, 17, and 18% CP for the sixth and seventh week. Body weight and feed conversion ratio were higher in T3 than the other treatments groups (p<0.05). Feed intake was higher in than males than females treatment group (p<0.05). No significant differences were observed in carcass percent and leg meats production rate was 22.5% in T1 treatment of male and female were 22.5% of the T5 treatment. In addition, 21.4% in females treated T4 and T6 treatment was 21.5%. Abdominal fat was higher in males than females. After seven weeks, the moisture content of litter did not differ among the treatments. In the experimental results, large male broilers at the end of the feeding in protein and energy levels should be increased. However, females did not suggest a need to increase.
We investigated the effect of glutathione supplementation on feed intake, body weight gain, velvet antler yields and economics of elk yearling. Fourteen elk yearlings were divided into 2 groups. Seven yearlings in each group (control and treatment) were fed with 1.5-2.5 percent body weight (%BW) of concentrate feed for elk, voluntary intake of hay as roughage and 6 mg/kg body weight (KBW) of glutathione. The results were as follows. Average daily gain (ADG) for control elk yearlings for first 1.5 month was 0.46 kg, while that of glutathione supplemented was 0.55 kg. Although glutathione fed group had higher ADG compared to control group (p<0.05), ADG after 1.5 month showed no difference. In spring, daily DM intake per elk yearlings was 3.98 and 4.24 kg for control and glutathione supplemented groups, respectively. The statistical differences in feed intake between two groups were not observed. However, feed intake tends to increase in glutathione fed group. Production of velvet antlers for elk yearlings were 911±256 and 1066±357 g for control and glutathione supplemented groups, respectively. Statistical difference between two groups was not observed due to the high variation. In economic analysis, additional 109,110 KRW per head for the cost of glutathione resulted in 2 percent higher revenue index due to the increased antler production. In conclusion, feeding glutathione to elk deers effectively increased DM intake and ADG of elk yearlings. Glutathione supplementation in feed might increase velvet antler production as well as ADG.
This study was carried out to elucidate changes in milk production, milk components and milk product properties as influenced by organic and conventional farming system. Milk production in transient organic feeding was lower by 7.6% than that in conventional feeding and also milk fat, milk protein content, solids not fat and somatic cell count were lower than conventional farming system. But, milk lactose content in transient organic feeding was higher than that in conventional feeding. Ca and Fe content in milk in organic feeding tended to be higher compared to conventional feeding. Linoleic acid and unsaturated fatty acids in milk in organic feeding was higher than those in conventional feeding and properties of milk products were improved in organic milk compared to conventional milk.
legumes and rye on paddy field at the experimental field of Jeollanam-Do Agricultural Research and Extension Services from 2004 to 2008. The five treatments used in this experiment were rye mono (C.V. Koolgrazer), rye+chinese milk vetch mixture, rye+crimson clover (C.V. Linkarus) mixture, rye+forage pea (C.V. Austrian pea) mixture, rye+hairy vetch (C.V. Oregon Common) mixture. The dry matter (DM) percentage at harvest was 23. 0~25.3%. The DM percentage among rye single and mixture treatments were similar. The yields of fresh and crude protein (CP) were high in Italian ryegrass (IRG)+Forage pea mixtures as a 50,370 kg and 1,177 kg per ha. And the yields of dry matter were high in IRG+forage pea was at mixtures as a 12,390 kg/ha. The Av. P2O5 in IRG+forage pea mixture was 85 ㎎/㎏. In conclusion, the mixture cultivation of rye and forage pea was very effective
The present study was performed to determine beneficial effects of donkey meat extract supplementation (15 and 30 mg/kg B.W.) on streptozotocin (STZ) induced diabetic rat. Rump muscle of donkey was extracted by boiling in water for 3 hrs and supplemented to STZ induced diabetic rats for 6 weeks. Feed and water intake of STZ induced diabetic rats were higher than those of normal rats, while no significant difference was found in DME treated rats as compared to the rats in control group. STZ-induced diabetic rats did not improved their body weight as compared to that of rats in the control group. However, blood glucose level of 30 mg DME supplemented rats was found to be significantly reduced after 4 weeks of supplementation (p<0.05) when compared to the control animals. Administration of DME did not show any improving effect on serum lipids (HDL-cholesterol, total cholesterol, and triglyceride). No significant changes were noticed in terms of weight of all the visceral organs of animals fed on DME except liver. It is therefore, concluded that supplementation of DME with dose of 30 mg/B.W. resulted in decreased blood glucose levels, however, blood lipid profile of diabetic rats, having more than 400 mg/dl glucose level, did not change.
Analysis of meat quality grade based on the DNA identity test samples were done on 320 Hanwoo (Korean cattle) raised in Gyeonggi region of Korea. 62.29% of cows and 91.35% of steers showed high quality which was above 1st beef grade. However, differences were observed between Gyeonggi Hanwoo and total slaughtered Hanwoo in Gyeonggi in the year 2009, though the sampling was entirely based high quality meat. Meat samples of pedigree registered cattle showed high rate of above 1st meat grade compared to advanced and fundamentally registered cattle. Pedigree registry is associated with quality grade of beef like the sex of cattle. Appearance rate of above 1st beef grade in offsprings of the top 9 Korean Proven Bulls produced through mating to Gyeonggi Hanwoo was 43.27% which was found to be higher than the average grade in cows (47.3%) and was 13.08% higher than the average grade in steers (77.49%). Our results showed superior genetic characters of Korean Proven Bulls which favors and influence the meat quality grade of their offsprings. Furthermore, our results claim that sex of Hanwoo irrespective of pedigreed or Korean Proven Bulls affects and/or influence the meat quality. Conclusively, the results could therefore be used as a basic data for the improvement of beef quality. However, paternity test by DNA typing and huge sample needs to be analysed to authenticate the findings.
The present study investigated the dealing mode and the quality of Chuncheon Dakgalbi on marketing. Distribution of chicken as raw material for Dakgalbi at 100 restaurants in Chuncheon was studied. Boneless chicken was sold at 89, bone-in chicken was sold at 9 and both of them were sold at 2 restaurants, respectively. Consumer's taste for Dakgalbi was surveyed at different area such as apartment (A), university (B) and downtown area (C) in Chuncheon. The redness and taste score of Dakgalbi was found to be significantly higher (p<0.05) among the members of family (A) as compared to others. Saccharinity and salinity of Dakgalbi were rated significant (p<0.05) among university student (B) than other consumers. In conclusion, quality characteristics of Dakgalbi differs with marketing area or consumer group in Chuncheon.