Background: Porcine embryonic development is widely utilized in the medical industry. However, the blastocyst development rate in vitro is lower compared to in vivo . To address this issue, various supplements are employed. Extracellular vesicles (EVs) play the role of communicators that carry many bioactive cargoes. Additionally, the contents of EVs can vary on the estrous cycle. Methods: We compared the effects of adding EVs derived from porcine uterine fluid (UF), categorized as non-EV (G1), EVs in estrus (G2) and EVs in diestrus (G3). After in vitro culture (IVC) was performed in three different groups, cleavage rate and blastocyst development rate were examined. In addition, glutathione (GSH) and reactive oxygen species (ROS) levels were measured 2 days after activation to assess oxidative stress. Results: Using NTA and cryo-TEM, we confirmed the presence of EVs with sizes ranging from 30 nm to 200 nm, that the particles were suitable for analysis for analysis. In IVC data, the highest cleavage rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Similarly, the highest blastocyst development rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Conclusions: These results indicate that estrus derived EVs contain biofactors beneficial for early blastocyst development, including GSH which protects the blastocyst from oxidative stress. Additionally, although diestrus-derived EVs are expected to have some effect on blastocyst development, it appeared to be less effective than estrus-derived EVs.

Extracellular vesicles (EVs) are nanovesicles that carry bioactive cargoes of proteins, lipids, mRNAs, and miRNAs between living cells. Their role in cellular communication has gained the attention of several research reports globally in the last decade. EVs are critically involved in sperm functions, oocyte functions, fertilization, embryonic development, and pregnancy. The review summarizes the state-of-theart of EVs research in the diagnostic and therapeutic (theranostic) potentials of the EVs during the pregnancy that might provide a solution for gestational disturbances such as implantation failure, maternal health problems, gestational diabetes, and preeclampsia. EVs can be found in all biological fluids of the fetus and the mother and would provide a non-invasive and excellent tool for diagnostic purposes. Moreover, we provide the current efforts in manufacturing and designing targeted therapeutics using synthetic and semi-synthetic nanovesicles mimicking the natural EVs for efficient drug delivery during pregnancy.

유전적 조절, 유전자 발현 그리고 환경적 단서, 화학적 신호에 대응하는 표현형 변이에서 세포 RNA는 ubiquitous 역할 이외에도 세포 외 RNA(exRNA)라 하는 새로운 형태의 RNA는 추후 연구의 방향을 제시한다. exRNA는 membrane vesicles 또는 세포 외 소포체(EV)로 알려진 membrane blebs를 통해 세포 외부로 운반된다. EV의 형성은 원핵생물, 진핵생물, 고세균을 포함한 모든 미생물군에 우세하게 보존되어있다. 본 리뷰는 세균 유래 exRNA 에 관해 세가지 주제에 초점을 두었다. exRNA의 발견과 박테리아 유전자 배열에 대한 외부 RNA의 영향, b. exRNA 의 분비기작을 통한 방출, c. 다른 그람음성 및 그람양성균에 의해 분비되는 exRNA로 고안될 수 있는 응용 가능 분야이다. 본 리뷰에서 장내 미생물군의 probiotics 및 후 성유전학적 규제에서 본 exRNA와 exRNA마커와 같은 EV 파생 응용프로그램에 대한 의견을 제공할 것이다.