The study conducted to understand the annual phenology of hemipteran insect pests including Halyomorpha halys, Nezara antennata, Plautia stali, and Riptortus pedestris in Jeju, Korea, 2017 and 2018. We investigated the population density of them at three locations for two years. The commercial and fish trap were used for monitoring. We compare the seasonal occurrence among species and sampling locations. The repeated measured ANOVA was used to explain the difference of seasonal occurrence among locations and sampling time. There was difference the first adult catch and the peak occurrence of species among sampling sites.

Insects are directly and/or indirectly associated with plants, and interactions of them have long been studied in many countries. The suborders Heteroptera and Auchenorrhyncha, in Hemiptera which is the largest hemimetabolous insect order, are also closely associated with various plants as their hosts and habitats. In addition, some groups of Auchenorrhyncha are known as the vector to transmit pathogens such as phytoplasma and virus to the plant. However, there have been a few researches on interaction of insect groups and plants, although it is important to construct data and to understand their interaction for application in various fields. In this talk, the current research on interaction of two suborders and plants is presented. The importance and direction of future work are also discussed.

In the midst of environments with crop-mixing, there were more preferred the fruit trees by brown-winged green (Plautia stali (Scott)), and yellow-brown stink bug (Halyomorpha halys (Stål) and vegetables by Far eastern green stink bug (Nezara antennata Scott) and Bean bug (Riptortus pedestris (Thurnberg)). The major dominant species was identified as P. stali, infesting fruit trees over 99.4% among tested insects. It is severely damaged on peach of middle and late ripening, crab apple, pear and wild fruit of Actinidia arguta. In an investigation for the seasonal occurrence using aggregation pheromone trap, P. stali was peaked with three times at late-May ~ early-June, mid- and late-July, and mid-Sept. ~ Oct. And H. halys slightly peaked at mid-June, and mid- and late-July and increased the occurrence at mid-Sept. ~ mid-Oct. Using seven insecticides - dinotefuran WP, flonicamid WP, etofenprox WP, chlorpyrifos WP, cabaryl WP, chlothianidin SC, and bifenthrin WP - registered on apple, contact and residual toxicity was tested on both male and female of P. stali and H. halys. Contact toxicity of dinotefuran WP was excellent on P. stali but the others had low effect. While all insecticides except flonicamid, residual toxicity were all effective on P. stali. Dinotefuran have a excellent contact toxicity and chlorpyrifos and bifenthrin have good residual toxicity against both male and female of H. halys.

The Riptortus-Burkholderia symbiosis is a newly emerging insect-bacterium symbiotic system. This symbiosis system has a good merit as an experimental model system to produce the non-symbiotic (apo) and symbiotic (sym) host insect. In recent reported papers, the symbionts play important biological roles for the host insects. Meanwhile, juvenile hormone (JH) is one of major hormone synthesized corpora allata(CA) to control many physiology of insect. However, the study for cross-talk mechanism between symbionts and host hormones to control important physiological phenomenon of insects is almost none. In this study, we found that Riptortus speed up adult emerging and increase egg laying on presence of symbiont Burkholderia. Also we found that hexamerin proteins, which were controlled the expression by JH, were accumulated in sym-Riptortus hemolymph compare with apo-Riptortus. According as combined results, we hypothesized that the gut symbiont Burkholderia can control JH titer to conclude out beneficial effects such as development and reproduction of R. pedestris. To verify this hypothesis, we examined measurement of JH titer, expression of hexamerins as JH response genes and RNAi for hexamerin protein during whole Riptortus life on presence or absence of symbiont Burkholderia. All results demonstrated that gut symbiont controlled JH titer of Riptortus. Controlled JH amount by symbiont Burkholderia in host midgut regulated hexamerin protein expression for speeding up adult emerging and increasing egg production.

In the midst of environments with crop-mixing, there were more preferred the fruit trees by brown-winged green (Plautia stali (Scott)), and yellow-brown stink bug (Halyomorpha halys (Stål)) and vegetables by Far eastern green stink bug (Nezara antennata Scott) and Bean bug (Riptortus pedestris (Thurnberg)). The major dominant species was identified as P. stali, infesting fruit trees over 99.4% among tested insects. It is severely damaged on peach of middle and late ripening, crab apple, pear and wild fruit of Actinidia arguta. In an investigation for the seasonal occurrence using aggregation pheromone trap, P. stali was peaked with three times at late-May∼early-June, mid- and late-July, and mid-Sept. ∼Oct. And H. halys slightly peaked at mid-June, and mid- and late-July and increased the occurrence at mid-Sept. ∼mid-Oct. Using seven insecticides - dinotefuran WP, flonicamid WP, etofenprox WP, chlorpyrifos WP, cabaryl WP, chlothianidin SC, and bifenthrin WP - registered on apple, contact and residual toxicity was tested on both male and female of P. stali and H. halys. Contact toxicity of dinotefuran WP was excellent on P. stali but the others had low effect. While all insecticides except flonicamid, residual toxicity were all effective on P. stali. Dinotefuran have a excellent contact toxicity and chlorpyrifos and bifenthrin have good residual toxicity against both male and female of H. halys.

경남 지역의 단감원에서 2000­2001년에 수은유아등과 2001년에 갈색날개노린재의 집합페로몬(methyl (E, E, Z)-2, 4, 6-decatrienoate)) 트랩을 이용하여 갈색날개노린재(Plautia stali)와 썩덩나무노린재(Halyomorpha halys)의 발생소장을 조사하였다. 유아등 조사결과 썩덩나무노린재는 6월 하순부터 발생하기 시작하여 8월 상ㆍ중순이 최성기이었다. 갈색날개노린재는 썩덩나무노린재와는 달리 유살 최성기가 뚜렷하지 않았는데, 主 유인시기는 2개년 모두 7월 중순­8월 하순이었다. 두 종 모두 9월 이후에는 거의 유살되지 않았다. 집합페로몬 트랩에 의한 유살 양상은 유아등에 의한 유살양상과 달랐다. 집합페로몬 트랩조사 결과, 두 종 모두 5월 중순부터 8월 하순까지 꾸준히 유인되었다. 썩덩나무노린재는 유인수가 적어 주 발생시기가 뚜렷하지 않았고, 갈색날개노린재는 지역에 따라 6월 하순부터 8월 하순 사이에 많이 유살되었다. 갈색날개노린재는 수은유아등 보다는 집합페로몬 트랩에, 썩덩나무노린재는 수은유아등에 더 많이 유인되었다. 또한 집합페로몬 트랩에는 두 종 모두 수컷보다 암컷이 더 많이 유인되었다. 두 종류의 트랩에 의한 유살소장 차이의 원인을 탄닌함량과 연관지어 고찰하였다.