This study was investigated to evaluate the effects of fertilizer application of surface and broadcast for rice culture on the soil chemical, physical, and microbial properties as well as growth and yield of rice. The application was made with ‘"Dongjin 1’" rice at Jeollanam-do Agricultural Research & Extension Services from 2008 to 2010. Soil organic matter and cation concentrations were increased by surface and broadcast applications, respectively. Plots treated by surface application tended to be higher seasonal N-mineralization rate in the organic fertilizer and seasonal soil organic matter than those of broadcast application. Soil physical properties seemed to be improved by the broadcast application, and soil microbial properties were increased by the surface application. Surface application increased 5% of rice yield compared to that of broadcast.