This studies were conducted to investigate the survival rate of frozen-thawed spermatozoa of Jindo Dog by monosaccharide and freezing rates. Experimental animals were prepared 12 males within 1~8 year's old and collected once in a couple of weeks by digital manuplation methods. Collected semen was diluted 1:1 with Tris-egg yolk extender and added 4, 6 or 8% of glycerol and none, 4 mM glucose or 4 mM fructose as cryoprotectant and was equilibrated for 2 hrs in . In monosaccharide groups, the freezing rate was 5 cm-5 min. above . The survival rates without monosaccharide were , , in 4, 6 or 8% glycerol, respectively. In addition of glucose, the survival rates were , , in 4, 6 or 8% glycerol, respectively and in fructose, were , , in 4, 6 or 8% glycerol, respectively. There showed significantly different between glycerol groups and monosaccharides groups (p<0.05). The survival rates of freezing rate in 5 cm-5 min. group was , , and in 10 cm-10 min. group was , , in 4, 6 or 8% glycerol, respectively. There were significantly different between freezing rates (p<0.05). These results suggest that the addition of fructose with 6%-glycerol and slow freezing improve the survival of frozen-thawed sperm in Jindo Dog.
Switchgrass is a wild-perennial plant in North America, and rich in cellulose, making it attractive as a source for cellulosic ethanol. Especially, ethanol which is made from switchgrass produce more 540% energy better than the others. The objectives of this study were to examine the concentrations of phenolic compounds, monosaccharides and disaccharides which detected in some different part (upper, center, lower) of 34 species of switchgrasses. The total average of phenolic compound concentration was 4017.48 ㎍/g. Whereas it's concentration in the upper part was shown the highest (6669.09㎍/g) and the lower part was the lowest (1916.30㎍/g). The No.23 has the highest concentration of phenolic compounds but No.20 have the lowest concentration. In saccharides analysis, only arabinose and glucose are detected. The total average of monosaccharides and disaccharides concentration was 206284.75 ㎍/g. Whereas its concentration in the upper part was shown the highest (269738.18㎍/g) and the lower part was the lowest (167567.53㎍/g). The No.30 has the highest concentration of saccharides but No.2 have the lowest concentration. The obtained results shows that switchgrass are good sources of bio-ethanol and the upper part is the best among them.
품종의 쌀을 시료로 하여 전분분자의 미세구조 및 전분입자 막 조성 탄수화물의 특성과 식미치와의 상관을 분석함으로써 밥맛에 영향을 미치는 탄수화물 성분 특성에 대해서 검토한 결과는 다음과 같다. 1. 본 연구에 사용한 8품종 가운데 식미치는 고품벼가 가장 높았다. 2. 아밀로그램상의 호화특성에도 유의미한 차이가 있었는데, 최고 점도(Peak viscosity)와 강하 점도(breakdown)는 히토메보레가 가장 높았고, 미향벼가 가장 낮았다. 그러나 이들 아밀로그램상의 호화특성과 식미치와는 아무런 상관이 없었다. 3. 전분분자 중의 아밀로오스 함량은 품종 간에 유의미한 차이가 있었고, 토요식미기에 의해서 분석된 아밀로오스 함량과도 차이가 있었다. 그러나 아밀로오스 분자의 구조적인 특성과 식미치 간에는 상관이 없었다. 4. 아밀로펙틴의 미세구조 중 B/A의 값이 가장 큰 품종은 새추청벼였고, 가장 작은 품종은 팔공벼였다. 그러나 이들 수치와 식미치와는 상관이 인정되지 않았다. 5. DSC에 의한 전분입자의 호화특성도 품종 간에 차이가 있었으며, 특히 호화엔탈피는 품종 간 차이가 크게 나타났다. 그러나 호화엔탈피 역시 식미치와는 상관이 없었다. 6. 쌀 배유전분분획의 헤미셀룰로오스성 다당을 구성하고 있는 단당류는 rhamnose, fucose, ribose, arabinose, xylose 등이었으며, 총 단당류 함량은 새추청벼가 가장 높았고, 팔공벼가 가장 낮았다. 그러나 팔공벼를 제외한 양질미에서는 식미치와 상관이 없는 것을 알 수 있었다.