The oriental fruit fly, Bactrocera dorsalis, is highly polyphagous pest that damages over 400 plant species, including commercially grown crops and fruits. It was first described in Taiwan and has since invaded Japan and China. In 2021, B. dorsalis was reported on Jeju Island in the Republic of Korea (ROK). To investigate its origin and understand its colonization pathway in ROK, the genetic characteristics were analyzed by using mitochondrial genes. The study analyzed the haplotypes and genetic differences among populations oriental fruit flies collected from Southeast Aisa, including Thailand, Taiwan, and Vietnam. The mitochondrial genes cytochrome oxidase subunit I (COI) and cytochrome B (CytB) were used for the analysis. The results showed that B. dorsalis had high levels of haplotype diversity among species. No major haplotype was found among populations. The present study enhances our understanding of the haplotype diversity of B. dorsalis in neighboring countries of ROK. This will expand our knowledge of the source of origin and invasive pathway for B. dorsalis.

Solenopsis invicta, known as the red imported fire ant, is an insect native to South America. This species was unintentionally introduced into Australia, New Zealand, several Asian countries, Caribbean countries, and the United States. It shows a high survival rate and settlement potential in human-habitable and non-living areas such as tropical rainforests, disturbed areas, deserts, grasslands, and roads. In Korea, invasions of red fire ants have been reported every year since 2017, and two invasions were discovered in 2023. Quarantine agency analyzing the haplotype and colony social type of S. invicta for surveillance and control. Population genetic analysis using Microsatellite Alleleic data of 66 loci to trace the origin of the invasion. Through research cooperation with the United States Department of Agriculture (USDA), we have received samples and expanded our genetic information database. This study analyzed genetic differences between 15 invasive populations and 44 reference groups. As a result of microsatellite analysis, the domestic invasive population showed a genetic structure similar to those in Guangzhou, China, and Florida, USA.

To identify sand crab Ovalipes punctatus populations and establish management units for each population, mtDNA COI regions were analyzed. As a result, the clade of O. punctatus in Korea were separated by two with a genetic distance of 0.17 – 2.08%, and there was no significant difference in the result of pairwise FST values representing genetic differentiation by sampling areas (p > 0.05). Also, no geographical separation found in the distribution of haplotypes and the results of the haplotype network. This result suggests that O. punctatus larvae were dispersed for a long time by the ocean current by suffering meroplanktonic period for 1 month, and increased the gene flow due to the development of the swimming legs for the increase in mobility. Therefore, in the results of mtDNA COI region analysis of O. punctatus in the East Sea, Yellow Sea, South Sea and East China Sea (Ieodo) of Korea, no clear intra-species differentiation was found.

최근 기온이 높아지면서 강원도 대관령 감자 재배지로 날아오는 진딧물 발생이 늘어나고 있다. 그 중 복숭아혹진딧물(Myzus persicae)은 전 세계적으로 분포하고 경제적으로 중요한 해충으로, 감자, 고추 등 노지작물에 흡즙을 통한 바이러스 매개하고 생육저하를 일으키는 피해를 주고 있다. 본 연구는 복숭아혹진딧물의 지리적 차이에 따른 집단의 유전적 관계 규명을 통해 기주식물, 메타집단 등의 군집구조 및 발생패턴을 분석하고, 이러한 요인으로 인한 진딧물 집단의 분산 및 이주 관계를 추정하였다. 최종적으로 개체들 간의 집단유전학적 관계규명을 통해 이들의 확산과 비래 양상을 규명하고자 한다.

세계화로 인해 교역 및 여행객의 증가로 외래 병해충의 유입이 증가하고 있고 이들의 유입 및 확산 경로 규명을 위하여 분자 유전학적 분석 방법이 이용되고 있는 실정이다. 본 연구는 외래 해충인 Reticulitermes kanmonensis의 유전적 특성을 분석하기 위한 18개의 초위성체마커(microsatellite)를 이용하여 총 16개 지역(전주, 김제1, 김제2, 군산1, 군산2, 서천, 완주1, 완주2, 완주3, 완주4, 익산1, 익산2, 논산, 일본1, 일본2, 일본3)의 흰개미 244개체를 분석하였다. 집단간 대립 유전자 패턴(allelic patterns)분석결과 일본3 지역의 유전적 다양성이 높게 나타났고 완주2 지역은 다른 지역에 비해 비교적 낮게 나타났다. 집단 간 유전적 거리(Genetic differentiation)는 평균적으로 0.163정도의 유전적 다향성 비율을 나타냈고, 서천과 군산2 지역이 0.048로 낮게, 서천과 일본2 지역이 0.442로 높게 나타나 서천과 군산2 집단이 유전적으로 가깝게 나타나고, 반면 서천과 일본2 집단과는 유전적으로 멀게 나타났다. STRUTURE 분석결과는 클러스터 집단 수가 6(k=6)일때 이상적인 집단 패턴을 나타내었고, 유전적 구조는 A그룹(전주, 김제1, 완주2), B그룹(서천, 군산1, 군산2), C그룹(완주1, 김제2), D그룹(논산, 익산1, 익산2), E그룹(김제1, 완주2, 완주3), F그룹(일본1, 일본2, 일본3)의 6가지의 패턴이 지역별로 분리되어 나타났다.

In the 21st century, the risk of the exotic pest being invaded is increasing due to the rise of trading activities. Oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), has been considered to very destructive invader as it is highly intrusive and has a wide host range. In this study, 2,299 samples were collected from 88 locations in 12 countries by quarantine and investigation. Among them, 608 individuals were used for COI DNA barcoding analysis based on Neighbor Joining method with P-distance model. Population genetics analysis was conducted for 510 individuals selected from 47 locations of 12 Southeast Asian countries using 15 microsatellite markers. COI DNA barcoding results showed that B. dorsalis did not support any clade as a geographical isolation but almost indicated mixed populations of each country. Population genetics analysis showed similar pattern of genetic structure between neighboring countries across borders. Most domestic quarantine groups were more similar in genetic structure to Taiwan, China and Thailand in the order of appearance.