We conducted quarantine insect species diversity monitoring using DNA barcoding with 517 lepidopteran samples that were obtained from quarantine inspections of foreign vessels entering Korea. The DNA barcode of each sample was treated as a molecular operational taxonomic unit (MOTU). For species delimitation and species identification of the analyzed samples, we applied a 2% cutoff rule and then identified with using BLAST of NCBI GenBank and BOLD System ver. 3.0. Consequently, 517 analyzed samples were delimited as 214 putative species across 20 families. Of these 214 putative species 145 (368 samples) were considered taxonomically identified if the closest BLAST match was no more than 2% different. Therefore the number of samples that were identified to the species level was relatively low, at approximately 71%. 115 of the 145 species were known in Korea. Of the 30 species that were not known in Korea, three, i.e., Noctua pronuba (Noctuidae), Orthosia hibisci (Noctuidae), and Pieris brassicae (Pieridae), were checked as ‘Regulated pests’ in Korea. We suggest that the three regulated pest species could be prevented from being introduced to Korea if monitoring of the vessels that pass the navigation route that contains these three species is performed consistently. Therefore, we suggest that the monitoring of quarantined insect pest enables the prevention of the introduction of alien species.

우리나라는 1996년 WTO/SPS(동식물검역협정)에 따라 검역병해충제도를 정비하였으며, 해외병해충의 위험으로부 터 자국내 생물자원과 자연환경을 보호하기 위하여 다양한 노력을 하고 있다. 현재까지의 수입식물 검역과정에서 검출된 병해충을 분석한 결과 병해충 검출건수는 수입량이 늘어나면서 증가하는 추세이다(00년 6,233건 → 17년 12,749건). 하지만 식물의 수입 유형에 따라 곡류, 사료류 등 비재식용 식물의 경우 병해충 검출률은 07년 이후 감소하고 있으며(07년 9.8% → 17년 3.3%), 묘목류, 종자류 등 재식용 식물의 경우 11년 이후로 지속적으로 증가하고 있어(11년 7.9% → 17년 22.0%) 재식용 식물을 통한 해외 병해충의 유입 위험도가 가증되고 있는 상황이다. 따라서 우리는 검역본부에서 운영하고 있는 자체 전산시스템인 병해충정보시스템(PIS)을 이용하여 2000년 이후 식물검역과 정에서 발견된 병해충 검출동향과 수입 유형에 따른 검출동향을 비교분석하고, 2016년 대비 2017년 병해충 검출동향을 파악하였다.

Craspedoxantha genus has been distributed in worldwide as 7 species in Afrotropical and 2 species in Oriental Regions. It is generally known that C. marginalis is preferred Asteraceae including Vernonia spp. for host plant. However, C. marginalis was first found in cut-flower of Phaenocoma prolifera, which was imported from South Africa, under the plant quarantine inspection in Korea. Therefore, we first report that P. prolifera as a new host plant for C. marginalis and provide information of the morphological characteristics and DNA barcoding sequences on male and female for identification.

We carried out DNA barcoding of five Korean Lymantria species to establish identification references library for quarantine inspection. Total of 118 samples including 34 samples obtained through quarantine inspection, two from USDA, and one collected from Philiphine were used for this study. And 30 sequences of 10 species from GenBank of NCBI were used as reference sequences. In a result of DNA barcoding of the Korean Lymantria species, sequence divergence of 148 DNA barcodes ranged from null to 17.0%, intraspecific divergence from null to 1.0%, and interspecific divergence from 5.1 to 17.0%. In NJ tree, L. dispar contained three clusters, which were identified as L. dispar asiatica, L. albescens, and L. xylina, respectively. L. xylina was collected through quarantine inspection on a foreign merchant ship in Yeosu port, and L. albescens was obtained by pheromone trap on L. dispar installed in Busan port. And L. monacha known as single species in Korea was revealed as species complex with three species, L. monacha, L. minomonis, and L. sugii. In subspecies level, L. dispar dispar (EGM) built single cluster, but L. d. asiatica (AGM) and L. d. japonica showed as multiple cluster. Therefore, DNA barcoding lead to rapid and accurate identification in species level, but in subspecies level, only a taxon showing geographically far distance was discriminated from the others. And the results could provide a taxonomic outline of the Korean Lymantria fauna and might be used as identification reference for Lymantria species in quarantine inspection.