전 세계적으로 급격하게 성장하는 스마트농업의 중요 요소 중 하나인 자동 관개시스템은 토양수분 센서에서 계측한 데이터를 기반으로 관개시점과 관개량을 판단하기 때문에 토양수분 센서의 설치가 필수적이다. 하지만 국내의 경우 토양을 고려하지 않고 단순히 포장의 가운데에 센서를 설치하는 등 토양수분 센서의 설치 위치에 대한 기준이 마련되어 있지 않아 토양수분 계측 위치에 관한 기준 검토가 필요하다. 본 연구에서 통계학적 방법을 이용하여 토양수분의 대표 계측지점을 선정 연구를 수행하였다. 토양은 수직적 또는 수평적으로 불균일성을 갖기에 구명이 쉽지 않다. 따라서 포장 전체에 걸쳐 지속해서 편향이 발생하지만 특정 위치에서의 평균 토양수분이 시간에 따라 유지한다는 시간 안정성 개념을 기반으로, 평균 토양수분을 나타내는 대표지점 선정 연구를 수행하였다. 토양수분을 측정하기 위한 시스템을 제작하였고, TDR (Time Domain Reflectometry) 센서를 이용하여 총 30개 지점을 측정하였다. 2023년 5월부터 8월까지 측정한 날짜·지점별 데이터를 이용하여, 지점의 편향을 정량화하여 식별할 수 있는 MRD (Mean Relative Difference, 평균상대차이)와 측정의 정밀도를 나타내는 RD (Relative Difference, 상대차이)의 SDRD (Standard Deviation of Relative Difference, 표준편차)를 산출하고, MRD와 SDRD를 통합한 지표로써 RMSE (Root Mean Square Error, 평균제곱근오차) 를 구하여, 시간 안정성이 가장 높은 지점인 RMSE의 수치가 최소인 지점을 대표지점으로 선정하였다. 토양수분 센서로 측정한 데이터를 사용하여 지점별 RMSE를 산출하고 비교하여, 평균적인 토양수분을 나타내는 대표지점을 선정할 수 있음을 확인하였다.

This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25°C), shaking extraction (SHE, 80°C), and sonication assisted extraction(SAE, , 25°C) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.

The objective of this study was to investigate the distribution of functional compounds in perilla leaves of various genetic resources and their antioxidant activities. A comprehensive analysis of functional compounds was conducted for 90 genetic resources, focusing on total polyphenol content (TPC), total flavonoid content (TFC), individual phenolic content (IPC), and lutein. Their antioxidant activities were then analyzed based on their radical scavenging capacity using ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The TPC content exhibited a range of 13.19 to 35.85 mg gallic acid equivalent/g, whereas the TFC content varied from 11.74 to 46.51 mg catechin equivalent/g. Total IPC was detected in a range of 6,310.98 to 40,491.82 μg/g. Lutein was detected at levels between 70.97 and 597.97 μg/g. ABTS and DPPH radical scavenging activities of perilla leaves ranged from 30.39 to 58.58 mg trolox equivalent (TE)/g and from 7.74 to 46.56 mg TE/g, respectively. Furthermore, correlation analysis demonstrated that rosmarinic acid, a phenolic acid, exhibited a significantly positive correlation with antioxidant activity. These findings suggest that various genetic resource of perilla leaves could effectively mediate antioxidant capacity. Results of this study provide valuable information for use of perilla leaves in Korea as functional food materials.

Until now, in case of Palaearctic region, only one species, Nesendaeus monochrous in this genus was recorded from China. but the biology and life cycle are not clearly known, yet. This species collected from the bud of Glochidion chodoense in Korea for the first time and closely related to Heterochyromera imerodeus Kojima & Morimoto which was recorded only in Japan,. But Nesendaeus monochrous is easily distinguishable from H. imerodeus by the relatively longer elytra with bisinuately round apices. Heterochyromera imerodeus Kojima & Morimoto can be found from Eurya emarginata and Glochidion obovatum in Japan. So, this two species are closely related each other and need more taxonomic study about the generic synonymy or changing the generic position.

This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.0104 cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 105 cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.

The consumption of ready-to-eat side dishes is rapidly growing in South Korea. These foods are particularly vulnerable to microbiological contamination as they are often cooked without any treatment, such as heating or stored at room temperature after cooking. Hence, in 2022, we analyzed the ready-to-eat side dishes sold in Gyeongsangnam-do, South Korea for microbiological contamination. We collected 100 samples from supermarkets in 7 cities, and then examined them for presence of food-borne pathogens and sanitary indicator bacteria. In the analysis of the food-borne pathogens, Bacillus cereus and Clostridium perfringens were isolated from 51 samples (51.0%) and 3 samples (3.0%), respectively. However, both quantitatively met the Korean Food Standards Codex. Genes of five different enterotoxins and one emetic toxin were analyzed from the 51 isolated B. cereus strains. We detected enterotoxin entFM (100.0%), nheA (94.1%), hblC (58.8%), cytK (56.9%), and bceT (41.2%) in 51 isolates, and emetic toxin gene, CER, in only one (2.0%) isolate. We did not detect C. perfringens toxin gene (cpe) that causes food poisoning in any one of the three C. perfringens isolates. In the case of sanitary indicator bacteria, Kimchi had the highest levels of total aerobic bacteria and coliforms, followed by Saengchae, Jeotgal, Jeolim, Namul, and Jorim, respectively. We counted total aerobic bacteria at two different storage temperatures (4oC and 20oC) to determine the effect of storage temperature. When stored at 20oC, total aerobic bacteria count increased in most of the ready-to-eat side dishes, except for Jeotgal. This result conclusively shows the need for refrigerating the ready-to-eat side dishes after purchase. Further research is needed to assess the risk and safety of the ready-to-eat side dishes available in the market and determine appropriate safety management practices.

Korea is a country where the population is concentrated in metropolitan areas that have undergone rapid industrial development. As of 2020, more than 43% of the total population lives in large cities, and about 18.5% of the total population lives in Seoul. A basic human need living in such a metropolis is a pleasant environment. In this study, complex odors and designated odors were evaluated at the boundary areas and at the outlets for 15 public environmental facilities selected from among odor sources in Seoul. As a result of measuring the complex odor intensity was 3 ~ 6 times at the boundary areas and 100 ~ 4,481 times at the outlets. In food waste treatment facilities, incineration facilities, and waste transfer station facilities, the compound making the largest contribution to odor is acetaldehyde, which was recorded at 46%, 25%, and 32% respectively. At a sewage treatment facility and agro-fisheries wholesale market, hydrogen sulfide was the largest contributing compound at 71% and 29% respectively.

Fermented foods of Korean temple are vegetable-based foods and their seasonings are limited to five pungent herbs. The aim of this study was to investigate cell-free supernatants (CFS) of lactic acid bacteria isolated from the fermented foods of Korean temples. Three selected CFS significantly inhibited the biofilm formation of Escherichia coli O157:H7 at 24 h, while one of the selected CFS markedly inhibited the biofilm formation at 72 h. However, only one CFS significantly attenuated the biofilm formation of Staphylococcus aureus at 24 h, but these CFS failed to inhibit the biofilm formation. Moreover, selected CFS effectively inhibited the growth of E. coli O157:H7, whereas the growth of S. aureus was not affected in the presence of CFS. These results suggest that CFS of lactic acid bacteria isolated from fermented foods of Korean temples shows antimicrobial activity agasint Gram-negative E. coli O157:H7, but not at Gramd-positive S. aureus .

The purpose of this study was to develop synbiotic materials and to apply them to the puffed grain products using electrostatic spraying technology. Lactic acid bacteria were isolated from Kimchi and selected through tests of acid resistance, bile salt resistance and γ-aminobutyric acid (GABA) content. The isolated Lactobacillus brevis CFM21 produced highest GABA production up to a concentration of 926.42 μg/mL when grown in MRS broth containing 0.8% MSG. The possibility of coated grains as a prebiotic material was evaluated Confocal laser scanning micro scopy (CLSM). Rice bran extract containing 2% dextrose, 2% soytone, 0.2% potassium chloride, 0.6% MSG was produced 524.77 μg/mL of GABA. Citrus sinensis oil showed the highest antibacterial activity against Clostridium perfringens Electrostatic spray showed much higher effectiveness than conventional spray in coating the puffed grain product through CLSM. Applying Rice bran culture and Citrus sinensis oil to puffed grain product using electrostatic spray could contribute to promote intestinal health of consumers.

Hepatic stellate cells (HSCs) play essential roles in normal and pathophysiological function in liver. In steady state, HSCs contribute to retinoid storage, immune tolerance, and extracellular matrix (ECM) homeostasis. Upon liver injury, they become activated and lead to morphological and functional changes. Studies have demonstrated that activation of HSCs by various stimuli such as toxins, microbial infection, or metabolic overload can promote the fibrotic changes in liver by production of ECM. Herein, we provide current knowledge about the basic characteristics of HSCs and the mechanism by which they are activated.

We investigated the physicochemical properties of brown rice by cultivar to select cultivar suitable for making brown rice porridge. The moisture content of the brown rice ranged from 8.79~11.78% with the highest varieties being ‘Geonyangmi’. The crude ash and crude lipid content ranged from 1.02~1.65% and 1.65~3.26%, while the rest were similar except for ‘Daebo’. Crude protein also had the lowest ‘Daebo’ and generally glutinous rice showed higher crude protein content than common rice. The hardness showed that ‘Seolgaeng’ and ‘Keunnun’ were the lowest, and ‘Haiami’ was the highest. In the RVA analysis, the setback was in the range －80.61~22.44 and was low in the order of ‘Wolbaek’, ‘Baekjinju'', and ‘Dongjinchal’. As a result of water binding capacity (WBC) measurement, ‘Sindongjin’, ‘Geonyang’ and ‘Samkwang’, were high in common rice, and ‘Dongjinchal’ and ‘Hwaseonchal’ were high in glutinous rice. Generally, solubility and swelling power in common rice was found to be lower than in glutinous rice. As a result, ‘Samkwang’ is considered suitable for brown rice porridge production because of high WBC, breakdown, and low setback.

The purpose of this study was to investigate antioxidant characteristics and anti-adipogenic effects of colored rice and brown rice extracts in 3T3-L1 adipocyte depending on cultivar (Josaengheugchal, Heugjinmi, Hongjinju, Geongganghongmi, Seolgaeng, Milyang 320, Sindongjin, Baegjinju). Colored rice and brown rice was extracted with 100% ethanol, followed by the analysis of polyphenol, flavonoid, anthocyanin, antioxidant, and anti-adipogenic activity. Total polyphenol and flavonoid content ranged from 6.86~314.08 mg GAE/g and 1.47~56.88 mg CE/g the highest total polyphenol and flavonoid content was observed in Heugjinmi cultivar. Anthocyanin composition was analyzed by HPLC, cyanidin-3-gluoside and peonidin-3-glucoside was found in black rice including Josaengheugchal and Heugjinmi. Also, the ABTS and DPPH radical scavenging activity of colored rice cultivars was higher than that of brown rice cultivars, the highest ABTS radical scavenging activity also was observed in Heugjinmi (128.20 mg TE/g). The anti-adipogenic effects of colored rice and brown rice extracts on differentiation of 3T3-L1 preadipocytes evaluated that extracts of Heugjinmi cultivar significantly reduced intracellular lipid accumulation. These results provide valuable information for the use of Korean colored rice cultivar as a functional food materials relative to anti-obesity.

정서조절은 정서와 관련된 개인차 요인이 개인의 정서 생활 및 행동에 미치는 영향을 매개하는 것으로 알려져 있다. 본 연구는 기존 매개모델에 상황 요인을 추가하여 상황에 따라 정서조절의 매개효과가 변화하는지 알아보았다. 이를 위해 다양한 직종에서 고객응대를 주 업무로 하고 있는 180명의 감정노동자를 대상으로 설문을 실시하여 감정 노동자의 일상적 부정정서, 인지적 재평가, 정서적 소진, 본인이 경험하는 고객 불만의 강도 등을 측정하였다. 조절된 매개효과를 알아보기 위하여 조건적 간접효과를 분석한 결과, 고객 불만의 강도가 높은 상황에서는 부정정서가 인지적 재평가를 매개로 하여 정서적 소진에 미치는 간접효과가 정적으로 유의하였으나 고객 불만의 강도가 낮은 상황에서는 해당 간접효과가 부적으로 유의하였다. 이는 높은 강도의 부정정서 유발 상황에서는 인지적 재평가의 매개효과를 통해 감정노동자의 정서적 소진이 감소될 수 있지만, 낮은 강도의 부정적서 유발 상황에서는 인지적 재평가의 매개효과를 통해 정서적 소진이 증가될 수도 있다는 것을 보여준다. 이러한 결과는 정서조절 연구에 있어 개인차적인 측면과 상황적 측면이 통합적으로 고려되어야 함을 강조하며, 감정노동자 직군의 정서적 특수성에 대한 정보를 제공한다.

The purpose of this study was to investigate the inhibitory effect of enzyme activity and anti-proliferation of human cancer cell lines (HCT 116, NCI-H460 and MCF-7) of peanut skin depending on cultivars (Arachis hypogaea L. cv. K-Ol, cv. Sinpalkwang, cv. Daan, cv. Heuksaeng) and extraction solvent. Peanut skin was extracted with 80% ethanol, 80% methanol, 80% acetone, and distilled water, followed by analysis of the enzyme inhibitory activity and anticancer activity. Methanol extract of Daan cultivar most effectively inhibited α-gluosidase (65.08%, 0.025 mg/mL), tyrosinase (82.49%, 2 mg/mL) and ACE (73.61%, 10 mg/mL). The inhibitory effect of peanut skin extracts on colon cancer cell (HCT-116), lung cancer cell (NCI-H460) and breast cancer cell (MCF-7) growth were investigate using MTT assay. The highest anti-proliferation of cancer cell line of peanut skin extracts was observed in the methanol extract of Daan cultivar. The cell viability on HCT 116, NCI-H460 and MCF-7 cell lines of methanol extracts from peanut skin of Daan cultivar was 48.13%, 41.03%, and 36.02% at 200 μg/mL, respectively. These results suggest that peanut skin extracts may mediate physiological activity, and provide valuable information for the use of peanut byproduct as a functional food material.