Background : The human patch test is a method used to evaluate potential skin irritation after contact with a cosmetic materials. Pectin lyase-modified red ginseng extract (GS-E3D) is a newly developed ginsenoside Rd-enriched ginseng extract. This study was designed to investigate the skin safety of GS-E3D in human patch test. Methods and Results : Thirty two female volunteers were tested with GS-E3D. GS-E3D (20 ㎕) was applied to occlusive patch test devices and was then applied onto the back of subject with normal skin for 24 hours. Cutaneous irritation responses were evaluated and graded according to criterion of International Contact Dermatitis Research Group (ICDRG) at 30 min, 24 hours, and 48 hours after removing of GS-E3D patch. The average age of subjects was 47.3 ± 9.3 years. Skin reactivity calculated from irritation score in GS-E3D treated group was 0.51 and skin irritation score of no application group was 0, respectively. Skin irritancy was no response in both GS-E3D treated group and no application group. From above data, GS-E3D was identified as a non-irritant according to ICDRG guideline that skin irritation score of ‘0.00 - 0.75’ is a non-irritant. Conclusion : These results indicate that GS-E3D can be useful as a safe cometic ingredient.