논문 상세보기
pp.747-756
본 연구는 흰쥐에게 AFB1을 투여하거나 방사선과 AFB1을 병합처리함으로 유발된 흰쥐의 간세포에서의 AFB1-DNA 부가체의 형성과 세포의 산화적 손상에 대한 vitamin C의 효과를 조사하기 위하여 수행되었다. X-ray 조사는 실험기간 내 단 1회로 실험사육기간 1일에 조사 하였고 X-ray 조사 후 vitamin C를 투여하였으며 vitamin C 투여 1시간 후 AFB1을 투여하였다. Vitamin C와 AFB1은 모두 복강투여로 실험 사육 첫 일부터 1회 시작하여 3일에 한번씩, 5회 반복 투였으며 실험동물 사육기간은 총 15일로 하였다. ELISA에 의한 흰쥐의 혈청 내 AFB1 잔여 농도는 AFB1 단독 투여군에서 5.17±0.34ng/mL이었으나 여기에 vitamin C 혼합 투여군에서는 3.23±0.76ng/ml가 검출되었다. 간세포의 AFB1-DNA adduct 농도는 AFB1 단독 투여군에서는 9.38±0.41ng/mL이었으며 2군에 vitamin C를 함께 투여한 3군에서는 5.28±0.32ng/ml로 나타나 2군에 비해 유의적으로(p＜0.001) 44% 감소한 양상을 나타내었다. 한편 X선 조사와 AFB1 병합처리한 4군에 비해 4군에 vitamin C를 투여한 5군에서 혈청 내 AFB1 함량과 간세포의 AFB1-DNA adduct 함량이 다소 감소하였으나 유의적인 차이는 없었다. 또한 면역조직화학적 관찰에서 AFB1 단독 투여군에서는 중심정맥과 혈관주변에서 AFB1 축적이 관찰되었는데 이러한 현상은 vitamin C를 혼합 투여함으로써 중심정맥과 혈관 주변의 갈색 침전이 현저하게 감소한 것으로 나타났다. 그러나 X선 조사와 AFB1 병합 처리한 군에서는 그 정도가 약했다.
The objective of this study was to examine the effects of vitamin C on the formation of aflatoxin B1 (AFB1)-DNA adduct and AFB1-induing cellular oxidative damage in rat livers treated with radiation and AFB1. Six-week-old male Sprague-Dawley rats were randomly divided into five groups: the control group, the AFB1-treated group, the group treated with AFB1 and vitamin C, the group treated with X-ray and AFB1, and the group treated with X-ray and AFB1 with vitamin C. On the first day of the experiment, only one dose of X-rays was exposed to the entire liver at 1,500 cGy. Next, vitamin C was injected at 10 mg/kg body weight via intraperitoneal injection, followed an hour later by the administration of 0.4 mg/kg of AFB1 via intraperitoneal injection. These treatments were administered every three days for 15 days. On the 16th day, the animals were sacrificed. The AFB1 contents of the rat sera were determined via indirect competitive ELISA. In the quantitative analysis of AFB1 in the rat sera via ELISA, 5.17±0.34ng/mL of AFB1 was detected in the AFB1-treated groups, but the amount decreased more significantly to 3.23±0.76ng/mL in the groups treated with AFB1 and vitamin C (p＜0.01) than in the AFB1-treated groups. The effect of vitamin C on AFB1-DNA adduct formation was determined via ELISA. The values of AFB1-DNA adduct formation were 9.38±0.41ng/mL in the AFB1-treated groups, but the amount decreased more significantly to 5.28±0.32ng/mL in the groups treated with AFB1 and vitamin C (p＜0.01) than in the AFB1-treated groups. Immunohistochemistry revealed that the accumulation of the AFB1 was not observed in the normal liver tissue (G1). The AFB1-positive materials were observed in the central vein and the portal vein of the liver tissue from the AFB1(G2) treatment or the X-ray and AFB1(G4) co-treatment, but the AFB1-positive materials were observed weakly in the group treated with vitamin C (G3 and G5). These results indicate that vitamin C had ameliorating effects on the AFB1 accumulation of liver tissue.
