This study was performed to develop and optimize a rapid strip kit for the dual-detection of Aflatoxin B1 (AF) and Ochratoxin A (OTA). The strip kit is composed of sample pad, conjugation pad, nitrocellulose membrane, and absorbent pad. Manually spotted mycotoxin-BSA conjugates and anti-mouse IgG on nitrocellulose membrane were used as the test line and the control line, respectively. Conditions for rapid and easy detection of mycotoxins, conjugation between toxin-MAbs and nano Au particles, pretreatment method of pads, and composition of standards solutions were optimized. Feasibility of the strip kit to detect AF and OTA were evaluated with standard samples. Test results were acquired in 10 min and the detection limits were 100 ppm for AF and OTA, respectively.

• 문지혜(국립농업과학원 농업공학부) | Jihea Moon
• 김기영(국립농업과학원 농업공학부) | Giyoung Kim Corresponding author
• 이상대(국립농업과학원 농업공학부) | Sangdae Lee
• 박샛별(국립농업과학원 농업공학부) | Saetbyeol Park
• 김혁주(국립농업과학원 농업공학부) | Hyeokju Kim