Candida albicans and their associated Candida species are opportunistic pathogens which exists as normal flora in the oral cavities of healthy individuals. In response to physiological changes in the host, these yeasts can become pathogenic, resulting in oral candidiasis. The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidiasis. The presently available culture and biochemical methods for detection and species identification of Candida are time-consuming and lack the required sensitivity and specificity. In this study, we have established a seminested PCR (snPCR) using universal and species-specific primers for detection of Candida species in saliva. The universal outer primers amplified the 3end of 5.8S ribosomal DNA (rDNA) and the 5end of 28S rDNA, including the internally transcribed spacer 2 (ITS2), generating 350- to 410-bp fragments from the four commonly encountered Candida spp., viz., C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis. The saliva from 331 healthy and, over 50 years of aged people lived in Dong-gu, Gwangu city, was collected. Total DNA were extracted by Hoffman-Winston yeast total DNA prep. method and performed t he s nP CR. R esults appeared to b e negative on 292 people ( 88.2%), however, 2 6 people ( 7.9%) were p ositive Candida albicans, 6 people (1.8%) were positive Candida glabrata, 5 people (1.5%) were positive Candida tropicalis, and only 2 person (0.6%) were positive Candida parapsilosis. These result showed that detection and identification of Candida species could be established by saliva analysis, so that snPCR on saliva is useful method of diagnosis of clinical fields

I. INTRODUCTION
 II. MATERIALS and METHODS
  1. Reference Organisms
  2. Saliva
  3. DNA Preparation
  4. PCR Primers
  5. DNA Amplification and Detection
  6. Antifungal Drug Sensitivity Testing
 III. RESULTS
  1. Standardization of snPCR
  2. Species Identification of Candida isolateson Saliva
  3. Antifungal Drug Sensitivity Testing
 IV. DISCUSSION
 V. CONCLUSIONS
 VI. REFERENCES

• 고영종(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Young Jong Ko
• 임원봉(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Won Bong Lim
• 김지선(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Ji Sun Kim
• 김인애(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | In Ae Kim
• 권혁일(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Hyuk Il Kwon
• 김옥수(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Ok Soo Kim
• 김병국(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Byungkuk Kim
• 김선미(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Sun Mi Kim
• 윤숙자(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Suk Ja Youn
• 강병철(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Byung Chul Kang
• 임회순(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Hoi Soon Lim
• 김미숙(Jeonnam Institution of Health and Environment) | Mi Sook Kim
• 김옥준(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Ok Joon Kim
• 최홍란(Dental Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) | Hong Ran Choi correspondence