A simultaneous determination was developed for 9 aminoglycoside antibiotics (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin, spectinomycin, and streptomycin) in meat by liquid chromatography tandem mass spectrometry (LC-MS/MS). Each parameter was established by multiple reaction monitoring in positive ion mode. The developed method was validated for specificity, linearity, accuracy, and precision based on CODEX validation guideline. Linearity was over 0.98 with calibration curves of the mixed standards. Recovery of 9 aminoglycosides ranged on 60.5~114% for beef, 60.1~112% for pork and 63.8~131% for chicken. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001~0.009 mg/kg and 0.006~ 0.03 mg/kg, respectively in livestock products including beef, pork and chicken. This study also performed survey of residual aminoglycoside antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Aminoglycosides were not found in any of the samples.

ABSTRACT
 재료 및 방법
  시료
  시약 및 재료
  표준원액 및 표준용액
  추출 및 정제
  LC-MS/MS 조건
  분석법 검증
  적용성 검증을 위한 잔류실태조사
 결과 및 고찰
  분석법 선정 및 조건 확립
  분석법 검증
  적용성 검증을 위한 잔류실태조사
 요 약
 참고문헌

• 조윤제(식품의약품안전평가원 식품위해평가부 잔류물질과) | Yoon-Jae Cho (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 최선주(식품의약품안전평가원 식품위해평가부 잔류물질과) | Sun-Ju Choi (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 김명애(식품의약품안전평가원 식품위해평가부 잔류물질과) | Myeong-Ae Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 김미경(식품의약품안전평가원 식품위해평가부 잔류물질과) | MeeKyung Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation) Correspondence to
• 윤수진(식품의약품안전평가원 식품위해평가부 잔류물질과) | Su-Jin Yoon (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 장문익(식품의약품안전평가원 식품위해평가부 잔류물질과) | Moon-Ik Chang (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 이상목(식품의약품안전평가원 식품위해평가부 잔류물질과) | Sang-Mok Lee (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 김희정(식품의약품안전평가원 식품위해평가부 잔류물질과) | Hee-Jeong Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 정지윤(식품의약품안전평가원 식품위해평가부 잔류물질과) | Jiyoon Jeong (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 이규식(식품의약품안전평가원 식품위해평가부 잔류물질과) | Gyu-Seek Rhee (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 이상재(식품의약품안전평가원 식품위해평가부) | Sang-Jae Lee (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation)