The objective of this study was to develop a simultaneous method of 8 penicillin antibiotics including amoxicillin, ampicillin, cloxacillin, dicloxacillin, nafcillin, oxacillin, penicillin G and penicillin V in meat using LC-MS/MS. The procedure involves solid phase extraction with HLB cartridge and subsequent analysis by LC-MS/ MS. To optimize MS analytical condition of 8 compounds, each parameter was established by multiple reaction monitoring in positive ion mode. The chromatographic separation was achieved on a C18 column with a mobile phase of 0.05% formic acid and 0.05% formic acid in acetonitrile at a flow rate of 0.2 mL/min for 20 min with a gradient elution. The developed method was validated for specificity, linearity, accuracy and precision in beef, pork and chicken. The recoveries were 71.0~106%, and relative standard deviations (RSD) were 4.0~11.2%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.003~0.008 mg/kg and 0.01~0.03 mg/kg, respectively, that are below maximum residue limit (MRL) of the penicillins. This study also performed survey of residual penicillin antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Penicillins were not found in all the samples except a sample of pork which contained cloxacillin (concentration of 0.08 mg/kg) below the MRL (0.3 mg/kg).

ABSTRACT
 재료 및 방법
  시약 및 재료
  표준원액 및 표준용액
  시료 전처리
  사용기기 및 분석조건
  분석법 검증
  적용성 검증을 위한 잔류실태조사
 결과 및 고찰
  분석법 선정 및 조건 확립
  분석법 검증
  적용성 검증을 위한 잔류실태조사
 요 약
 참고문헌

• 김명애(식품의약품안전평가원 식품위해평가부 잔류물질과) | Myeong-Ae Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 윤수진(식품의약품안전평가원 식품위해평가부 잔류물질과) | Su-Jin Yoon (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 김미경(식품의약품안전평가원 식품위해평가부 잔류물질과) | MeeKyung Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation) Correspondence to
• 조윤제(식품의약품안전평가원 식품위해평가부 잔류물질과) | Yoon-Jae Cho (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 최선주(식품의약품안전평가원 식품위해평가부 잔류물질과) | Sun-Ju Choi (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 장문익(식품의약품안전평가원 식품위해평가부 잔류물질과) | Moon-Ik Chang (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 이상목(식품의약품안전평가원 식품위해평가부 잔류물질과) | Sang-Mok Lee (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation,)
• 김희정(식품의약품안전평가원 식품위해평가부 잔류물질과) | Hee-Jeong Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation,)
• 정지윤(식품의약품안전평가원 식품위해평가부 잔류물질과) | Jiyoon Jeong (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation,)
• 이규식(식품의약품안전평가원 식품위해평가부 잔류물질과) | Gyu-Seek Rhee (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 이상재(식품의약품안전평가원 식품위해평가부) | Sang-Jae Lee (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation)