In this study, the virucidal efficacy of a fumigant (35% paraformaldehyde) against avian influenza virus (AIV) was examined. After AIV suspensions had been deposited on stainless steel carriers, the dried carriers were exposed to the fumigant in a 300-m3 test room for 3 h. Thereafter, all carriers were submerged in a neutralizing solution to scrape off the surviving viruses, and the respective suspensions were diluted. Each dilution factor was respectively inoculated into the allantoic membrane of five 10-day-old embryos. After incubation, AIV viability in the collected allantoic fluids was examined and the EID50 was calculated. The fumigant inactivated ≥5.7log10EID50 of AIV and was nontoxic to the embryos.

• Chun-Nam Cha(Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
• Eun-Kee Park(Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University)
• Chang-Yeul Yoo(Department of Smart Information Convergence, Gyeongnam Provincial Namhae College)
• Ji-Youn Jung(Department of Companion and Laboratory Animal Science, Kongju National University)
• Song-Ee Son(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Hu-Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author