A 26-month-old male mixed-breed dog of Korean origin was subjected to necropsy following death after a history of decreased appetite and weight loss. Necropsy revealed generalized lymphadenopathy and splenomegaly. Histopathological examination of samples from the spleen, mandibular lymph nodes, liver, kidney, and large intestine showed granulomas with numerous macrophages containing intracytoplasmic Leishmania amastigotes. Transmission electron microscopy revealed Leishmania amastigotes in the macrophage cytoplasm. All tissues with granulomas were positive for Leishmania spp, which was confirmed to be Leishmania infantum by conventional polymerase chain reaction (PCR), real-time PCR, and in situ hybridization. To our knowledge, this is the second case of canine visceral leishmaniasis in Korea.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Clinical signs and symptoms
  Necropsy and histopathological examination
  PCR and real-time PCR
 RESULTS
 DISCUSSION
 REFERENCES

• Hyunkyoung Lee(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• Kyung-Hyun Lee(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• Jae-Won Byun(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• Soon-Seek Yoon(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• Jung-Won Park(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• MyoungHeon Lee(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• Eun-Jin Choi(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• ByungJae So(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
• Dae-Young Kim(Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri)
• Matti Kiupel(Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, MichiganStateUniversity)
• Ingeborg M. Langohr(Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, MichiganStateUniversity)
• You-Chan Bae(Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) Corresponding Author