The objective of this study was to determine the efficacy of ozone in sanitizing water experimentally inoculated with the gram-positive food-poisoning bacterium Staphylococcus aureus. The bactericidal effect was measured after experimentally inoculated solutions were exposed to 0, 0.5, and 1.0 ppm ozone at several time points and different temperatures, in the presence of varying concentrations of different organic matter, namely, fetal bovine serum (FBS) or cattle liver. Results revealed inhibition of the bactericidal effect in the presence of the lowest percentage of FBS, but a lower extent of the inhibition occurred when liver was used as the organic matter. It was also apparent that a higher temperature and shorter ozone exposure time had led to a more reduced bactericidal efficacy than that under a lower temperature and longer ozone exposure. This study provides insight into the potential use of ozonated water as an effective and safe disinfectant in an abattoir setting.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Bacterial strain and growth condition
  Preparation of organic matter
  Preparation of ozonated water
  Statistical analysis
 RESULTS AND DISCUSSION
  Bacterial reduction rate
  Efficacy of ozonated water in preventing bacterialgrowth upon elimination of organic matter and atdifferent incubation times
  Effects of organic matter on the bacterial reduction rate
  Effects of different temperatures on the rate of bacterialkilling
 REFERENCES

• Lauren Togonon Arayan(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Alisha Wehdnesday Bernardo Reyes(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Huynh Tan Hop(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Huy Tran Xuan(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Institute of Agriculture and Life Science, Gyeongsang National University) Corresponding Author
• Hong Hee Chang(Institute of Agriculture and Life Science, Gyeongsang National University)
• Han Sul Yang(Institute of Agriculture and Life Science, Gyeongsang National University)