A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH4NO3, 0.3% KH2PO4, 0.3% K2HPO4, 0.02% MgSO4ㆍ7H2O, 0.0025% CaCl2ㆍ6H2O, 0.0015% FeSO4ㆍ7H2O in 1ℓ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 ㎖ of the same medium in 250 ㎖ flask. They were cultivated at 30 ℃ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.

• 차미선(부산대학교 미생물학과) | Mi-Sun Cha
• 임은경(부산대학교 미생물학과) | Eun-Gyoung Lim
• 이근희(부산대학교 미생물학과) | Keun-Hee Lee
• 조순자(부산대학교 미생물학과) | Sun-Ja Cho
• 손홍주(밀양대학교 생물공학과) | Hong-Joo Son
• 이상준(부산대학교 미생물학과) | Sang Joon Lee Corresponding Author