양손 협응을 통한 상지의 정상적인 운동은 일상생활동작 수행의 질을 결정하는 필수조건이 된다. 그러나 뇌졸중으로 인한 신경학적 손상은 뇌손상이 일어난 반대측 신체에 감각운동적 기능장애를 일으키게 된다. 마비측 상지 훈련뿐만 아니라 양측 상지의 동시적 운동 수행이 마비된 상지의 기능회복에 미치는 영향에 대한 연구가 활발히 진행되고 있다. 그러나 양측 상지 훈련을 다양한 기능 수준의 환자군에게 적용한 연구가 없다. 다양한 양측 상지 훈련 개발을 위해 양측 상지를 이용한 과제 에 대해 정확한 분석이 이루어져야 할 것이며 치료 효과를 이어갈 수 있도록 양측 상지 훈련에 대한 홈 프로그램 운동이나 프로토콜 개발이 필요할 것이라고 생각된다.

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC 381T. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was 25℃, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides (1.0 × 104 cells/㎖) cultures, over 98% of C. polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at 121℃ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at 121∼180℃ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28～32 ℃, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 % agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

The isolated strain, Rhodococcus sp. EL-GT was able to degrade high phenol concentrations up to 10 mM within 24 hours in the medium consisting of 5.3 mM KH2PO4, 95 mM Na2HPO4, 18mM NH4NO3, 1mM MgSO4·7H2O, 50μM CaCl2, 0.5μM FeCl3, initial pH8.0, temperature 30℃ in rotary shaker at 200rpm. This strain was good cell growth and phenol degradation in the alkaline pH range range, and the highest in the pH range of 7 to 9. The microorganism was able to grow at the various chlorinated phenols, benzene, toluene, and bunker-C oil. As Rhodococcus sp. EL-GT was good capable of attachment on the acryl media, it would be used as microorganism to consist of biofilm in wastewater treatment.

A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH4NO3, 0.3% KH2PO4, 0.3% K2HPO4, 0.02% MgSO4ㆍ7H2O, 0.0025% CaCl2ㆍ6H2O, 0.0015% FeSO4ㆍ7H2O in 1ℓ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 ㎖ of the same medium in 250 ㎖ flask. They were cultivated at 30 ℃ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.

An antimicrobial substance-producing microorganism was isolated from soil samples. Based of the taxonomic characteristics of its morphological, cultural, physiological properties and 16s rRNA sequence alignment, this microorganism was identified as Pseudomonas aeruginosa, and we named Pseudomonas aeruginosa EL-KM. The optimal culture condition for production of antimicrobial substance was 1% mannitol, 0.4% yeast extract, 0.5% Nacl, 0.2% K₂SO₄, 100μM MgSO₄.7H2O, 10μM CaCl2.$2H_2O$, 1μM $FeSO_4$.7H2O, 1μM MnSO4.$4-5H_2O$, initial pH 7 and 200 rpm at 30℃. The purification of the antimicrbial substance was performed by silica gel column chromatographys, and fraction with TLC $R_f$ 0.77 value represented good antimicrobial activity. The crude antimicrbial substance was stable within a pH range of 3-10 and temperature range of 4°C-121°C autoclaving. This crude antibacterial substance acted as bacteriolytic agent against Vibrio cholerae non-Ol ATCC 25872, and also exhibited excellent properties, when the substance was demonstrated against many other gram-positive, gram-negative bacteria, yeast and fungi.

The toxicity values of various heavy metals were evaluated by acute immobilization and chronic reproduction impairment tests, using Daphnia magna. Acute tests were evaluated by the inhibition of their mobilization after 24hrs without food addition. The tests of reproductive impairment were investigated for 21 days by food addition and exchange of water. The effect of each concentration was assessed by Probit analysis and t-test. The results obtained from this study were as follows : 1) The change of pH and DO was not significant in the acute tests, while, in the reproductive tests, pH was increased by 0.3∼1.4 and DO also increased. 2) The EiG50 values of immobilization to Daphnia magna in artificial fresh water were 0.030㎎/ℓ(Cu), 0.054㎎/ℓ(Cd), 0.12㎎/ℓ(Cr), 0.74㎎/ℓ(Pb), 3.4㎎/ℓ(As) and the NOEiC values were 0.010㎎/ℓ(Cu), 0.018㎎/ℓ(Cd), 0.010㎎/ℓ(Cr), 0.10㎎/ℓ(Pb), and 1.8㎎/ℓ(As). 3) The EiC50 values of reproductive impairment to Daphnia magna were 13.8㎍/ℓ(Cu), 2.9㎍/ℓ(Cd), 15.5㎍/ℓ(Cr), 61.7㎍/ℓ(Pb), 759㎍/ℓ(As), and NOErC values were 0.95㎍/ℓ(Cu), 0.54㎍/ℓ(Cd), 1.2㎍/ℓ(Cd), 7.4㎍/ℓ(Pb), 110㎍/ℓ(As). The results of tests using OECD artificial culture water were more sensitive than natural water for culturing. The presented data show that an artificial culture water is suitable in the experiment of bioassay for assessing the toxicity of materials.

Microorganisms producing bioemulsifier were isolated from the sea water in Pusan coastal area. The isolated strain which had the highest emulsification activity and stability was identified as the genus Acinetobacter from the results of morphological, cultural and biochemical tests and named Acinetobacter sp. EL-C6 for convenience. The compositions of optimum medium for emulsification of crude oil by Actnetobacter sp. EL-C6 were crude oil 2.0%, NH_4NO_3 0.2%, K_2HPO_4 0.01%, MgSO_4·7H_2O 1.0%, CaCl_2·2H_2O 0.1% and NaCl 3.0% at initial pH 7.5 and 30℃, respectively. The cultivation for emulsification of crude oil was carried out in 500㎖ shaking flask containing 100㎖ of the optimum medium at 30℃. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Acinetobacter sp. EL-C6 showed that utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, crude oil was best utilized by the Actnetobacter sp. EL-C6.