간행물
한국환경과학회지 KCI 등재 Journal of Environmental Science International
- 발행기관 한국환경과학회
- 자료유형 학술지
- 간기 월간
- ISSN 1225-4517 (Print)2287-3503 (Online)
- 수록기간 1992 ~ 2023
- 주제분류 자연과학 > 자연과학일반 자연과학 분류의 다른 간행물
- 십진분류KDC 539DDC 628
권호리스트/논문검색
제11권 제1호 (2002년 1월) 10건
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An antimicrobial substance-producing microorganism was isolated from soil samples. Based of the taxonomic characteristics of its morphological, cultural, physiological properties and 16s rRNA sequence alignment, this microorganism was identified as Pseudomonas aeruginosa, and we named Pseudomonas aeruginosa EL-KM. The optimal culture condition for production of antimicrobial substance was 1% mannitol, 0.4% yeast extract, 0.5% Nacl, 0.2% K₂SO₄, 100μM MgSO₄.7H2O, 10μM CaCl2.$2H_2O$, 1μM $FeSO_4$.7H2O, 1μM MnSO4.$4-5H_2O$, initial pH 7 and 200 rpm at 30℃. The purification of the antimicrbial substance was performed by silica gel column chromatographys, and fraction with TLC $R_f$ 0.77 value represented good antimicrobial activity. The crude antimicrbial substance was stable within a pH range of 3-10 and temperature range of 4°C-121°C autoclaving. This crude antibacterial substance acted as bacteriolytic agent against Vibrio cholerae non-Ol ATCC 25872, and also exhibited excellent properties, when the substance was demonstrated against many other gram-positive, gram-negative bacteria, yeast and fungi.
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