In this study, the condition of the hazardous materials in the bus was monitored according to the ventilation mode of the air conditioning system during bus service. The bus was surveyed using the indoor air quality measurement method of public transportation vehicles within one year of delivery. We evaluate the CO2 and PM10, which are the controlled parameters in buses by the Ministry of Environment, and VOCs and HCHO, the non-controlled parameters. The PM10 concentration increased due to outdoor air intake; however the CO2 concentration was found to decrease. In addition, the concentration of VOCs and HCHO was found to decrease due to the forced ventilation system and the outdoor air intake. These results show that the concentration of the other materials except PM10 can be changed due to the outside air concentration and forced ventilation system. Therefore, through indoor air quality characteristics of the bus according to air condition system are intended to be used as the basis of an operation manual.