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pp.8-13
A strain of Saccharomyces cerevisiae BY-366was found to produce a strong sucrose-hydrolyzing enzyme Using this strain, the optimal culture conditions for the production of invertase were investigated. The results are as follows : 1. For enzyme production, optimal temperature, initial pH and critical concentrations of sucrose and raffinose were 30℃, 5.0 and 3.0%, respectively. 2. Enzyme production was reached maxium by organic nitrogen source, 0.3% yeast extract plus 0.5% bactopeptone. 3. It was appeared the presence of 0.1M Mn^2+ and Fe^2+ ion was essential factors, on the other hand, 0.1M Ag^+ and Hg^2+ ion almost block in yeast growth and enzyme production. 4. Invertase productivity was reached maxium within 3days on stationary culture with medium-composed of sucrose 3%, bactopeptone 0.5%, yeast extract 0.3%, K_2HPO_4 0.1%, MgSO4·7H_2O 0.05%.
