논문 상세보기
pp.1-5
E. coli P90C 배양액중의 phosphatase는 ATPase로 대표될 수 있고 phosphatase 활성을 측정하기 위해서는 반응액과 30분이상 반응시켜야 안정된 발색도를 나타내었다. β-galactosidase의 생산을 위해 전배양에서 본배양으로 접종하는 시기는 phosphatase의 활성이 급증하기 직전(3hr)이 가장 좋았으며, 본배양에서 phosphatase 활성은 대수증식기에서 최고였으며, β-galactosidase가 생성되기 시작하면 phosphatase의 활성이 떨어지기 시작하는 것으로 나타났다.
ATPase was the most available phosphatase in culture broth of E. coli P90C. To measure the stable phosphatase activity it was necessary to react with reaction reagent over 30min and then we can get stable optical density at 410nm. Transfer time from preculture to main culture for the production of β-glactosidase was good after 3 hrs cultivation. Phosphatase activity was highest at log phase in main culture and as the cell begins to make β-galactosidase phosphatase activity begins to decrease.
