Background: Various intervention methods are being used to treat subacromial impingement syndrome. However, there is a lack of research on intervention using manual therapy and kinesiotaping together. Objectives: To investigated the effect of subacromial impingement syndrome on the mobilization with kinesiotaping. Design: A quasi-experimental clinical trial. Methods: An experiment was conducted by allocated twenty-nine patients with shoulder impingement syndrome to the mobilization with kinesiotaping group (MKG, n=15) and the kinesiotaping group (KG, n=14). The intervention of MKG and KG was conducted 3 times a week for 6 weeks. The outcome was The Shoulder Pain and Disability Index (SPADI) and range of motion (ROM). The collected data was analyzed using the SPSS ver. 21.0 program by paired t-test and independent t-test. Results: After the intervention, MKG had significant improvements in SPADI pain, SPADI disability, external rotation and internal rotation in MKG. However In KG, there was no significant decrease in SPADI pain, SPADI disability, external rotation and internal rotation. And in MKG, there was a statistically significant decrease in SPADI pain, SPADI disability and increase in external rotation and internal rotation. Conclusion: The mobilization with kinesiotaping was effective in improving pain, disability, and ROM in patients with subacromial impingement syndrome.