Along with the increase in the number of vehicles in circulation, the indoor air quality in automobiles is attracting attention as another possible health concern. However compared to data regarding indoor air quality in other spaces, there are insufficient data on indoor air quality in automobiles. In addition, there is no standard for the evaluation method. In this study, the change in the concentration of particulate matter in the vehicle while driving under real road conditions was analyzed in order to use it as basic data for a method to evaluate vehicle indoor air quality. Through the selection of measurement target materials and test vehicles and the preparation of test methodologies, evaluation was performed on vehicle, route, and HVAC modes. The concentration of particulate matter in the vehicle was the lowest in the RC (In-vehicle recirculation) condition, and it was confirmed that it decreased with time. The highest average concentration was confirmed in the OA (Outside air ventilation) condition, and the concentration change according to the changing HVAC mode was observed in the Auto condition. The concentration of pollutants inside the vehicle showed a significant correlation with factors such as season, external concentration, and HVAC conditions, along with a weak correlation to powertrain type. The results of this study can be used as basic data for developing methods for evaluating vehicle interior air quality in future work.