This study investigated the wetland plant community in Mije Reservoir, Gunsan City, during September and October 2024, emphasizing its ecological significance as a primary producer and habitat provider within agricultural reservoir ecosystems. The reservoir was divided into 14 distinct sectors to comprehensively assess spatial plant distribution patterns. Aquatic plants were sampled using collection anchors deployed at the water’s edge, while hygrophytes and emergent macrophytes were surveyed along transects extending from the water’s edge to the embankment. A total of 192 vascular plant species were identified, of which 73 were classified as wetland plant species, encompassing diverse life forms: 36 hygrophytes, 17 emergent macrophytes, 6 floating-leaf species, 3 free-floating species, and 11 submerged species. Notably, the survey documented the presence of two endangered species, Euryale ferox and Cicuta virosa, alongside eight invasive alien plant species. A comparative analysis with a prior survey conducted in 2008 revealed a substantial increase of 54 wetland plant species. Currently, Mije Reservoir is subject to intensive aquatic plant removal management due to observed excessive proliferation and cyanobacterial bloom. However, the presence of endangered species underscores the critical need for a more nuanced reservoir vegetation management strategy that integrates the spatial distribution and conservation status of these ecologically important wetland plants.