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반응표면분석법을 이용한 에틸락테이트 용매 기반 주황색 고구마 기능성 성분 추출 최적화 연구 KCI 등재

Optimization of Functional Compound Extraction from Orange-Fleshed Sweet Potato using Ethyl Lactate as a Green Solvent through Response Surface Methodology

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

This study optimized the extraction of β-carotene and antioxidant compounds from orange-fleshed sweet potato (Ipomoea batatas L.) using response surface methodology (RSM) with ethyl lactate as a green solvent. A central composite design (CCD) was utilized to evaluate how the solvent-to-sample ratio, temperature, and extraction time affected efficiency. The regression model was statistically significant (Adj. R2=0.67), revealing that the solvent-to-sample ratio was the most influential factor, while temperature and time had relatively minor effects. The optimal conditions identified were a ratio of 12 mL/g, a temperature of 35°C, and an extraction time of 30 minutes, which resulted in a yield of 27 mg/100g of β-carotene, along with high levels of polyphenols, flavonoids, and strong antioxidant activities as measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid)) assays. Validation experiments demonstrated a close alignment between predicted and experimental values, confirming the model's robustness. These findings indicate that ethyl lactate effectively extracts not only carotenoids but also a wide range of antioxidants, underscoring its potential as a sustainable solvent for developing functional food ingredients.

목차
Abstract
서 론
재료 및 방법
    1. 실험재료
    2. 실험설계
    3. β-Carotene 정량 분석
    4. 총 폴리페놀 및 플라보노이드 함량 분석
    5. 라디칼 소거능 측정
    6. 통계분석
결과 및 고찰
    1. 반응표면분석법(RSM)을 이용한 최적 추출조건 확립
    2. 기능성 성분 및 항산화 활성
요약 및 결론
감사의 글
References
저자
  • 장가은(농촌진흥청 국립식량과학원 전문연구원) | Gaeun Jang (Master's Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 남상식(농촌진흥청 국립식량과학원 전문연구원) | Sang-sik Nam (Master's Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 유경단(농촌진흥청 국립식량과학원 농업연구사) | Gyeong-dan Yu (Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 박유진(농촌진흥청 국립식량과학원 농업연구사) | You-jin Park (Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 김태화(농촌진흥청 국립식량과학원 농업연구사) | Tae Hwa Kim (Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 이교휘(농촌진흥청 국립식량과학원 농업연구사) | Kyohwui Lee (Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 박원(농촌진흥청 국립식량과학원 농업연구사) | Won Park (Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 안종욱(농촌진흥청 국립식량과학원 농업연구사) | Jonguk An (Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 황엄지(농촌진흥청 국립식량과학원 농업연구사) | Eom-ji Hwang (Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea) Corresponding author
  • 우관식(농촌진흥청 국립식량과학원 농업연구관) | Koan Sik Woo (Senior Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 이형운(농촌진흥청 국립식량과학원 농업연구관) | Hyeong-Un Lee (Senior Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)
  • 정미남(농촌진흥청 국립식량과학원 농업연구관) | Mi Nam Chung (Senior Researcher, National Institute of Crop and Food Science, Rural Development Administration, Muan 58545, Korea)