Fusarium head blight (FHB), caused by Fusarium graminearum is a major disease problem on wheat and barley in Korea. The objectives of this study were to evaluation of korean wheat cultivars for Type I and Type II resistance to FHB. We screened for Type II resistance in the greenhouse using single floret inoculation and for Type I resistance in the field using spray inoculation. Sumai 3 was used the FHB resistant check. Thirty-two korean wheat cultivars were evaluated for resistance to spread of symptoms within spike. The 2013 field screening with wheat cultivar was located in Kimjae-si Joeonbuk Korea. All plots were inoculated twice. Mist-irrigation was applied to facilitate FHB development. FHB severity was assessed visually 21 days after inoculation on 20 arbitrarily selected spikes per plot. FHB severity was determined as the percentage of symptomatic spikelets from the total of all spikelets. For FHB resistance, the average of FHB severity of Type I exhibited ranging from 21.9% to 77.2% and FHB severity of Type II ranging from 20.8% to 100%. Namhae, Milseong, Geuru, Joen, Anbaek and Sukang were the moderately resistant cultivars while Gobun, Alchan, Dajoong, Eunpa, Shinmichal1, Eunpa and Uri were the most susceptible cultivars for Type II resistance.

Tsw, a single dominant resistant gene against Tomato spotted wilt virus (TSWV), has been mapped on chromosome 10 in Capsicum chinense species. Previously reported molecular markers linked to the Tsw gene are not transferable for all pepper breeding materials. To develop additional markers and do genome-based fine mapping of the Tsw gene, approaches of mapping comparison, pooled transcriptome analysis, and genome walking were applied. Eleven additional SNP molecular markers tightly linked to the Tsw gene were developed using tomato and pepper whole genome sequencing databases. Among them, four SNP markers, SNP7715-1, SNP68-1, SNP17918-1, and SNP1072-1, showed no recombination in two segregating populations of F2 ‘Telmo’ (210 individuals) and ‘SP’ (843 individuals). Three scaffold sequences from the C. annuum BAC database and two BAC clones from the BAC library of C. annuum ‘CM334’ covering the Tsw gene were identified by transcriptome analysis and genome walking. A pepper scaffold sequence covering three pepper scaffold sequences was identified from a final version of the C. annuum BAC database. The Tsw gene was delimited within 149 kb by alignment analysis of two BAC clone sequences and the pepper scaffold sequence. A total of 22 predicted genes were resided in the target region between SNP7715-1 and SNP1072-1 co-segregating markers. Among them, five predicted genes showing annotations of CC/TIR-NBS-LRR resistance proteins, mRNA-6, mRNA-7, mRNA-11, mRNA-12, and mRNA-13, were identified. The transcriptome analysis and gene expression study showed that the mRNA-13 was expressed in ‘PI152225’ but was absent in ‘Special’, demonstrating the mRNA-13 could be a strong candidate gene for the Tsw gene. This result will be favorable for cloning the Tsw gene and developing cultivars which carry the TSWV-resistance gene.

The Brown planthopper (Bph) is one of the most serious pests of rice affecting rice yield and quality throughout the country. Combining the Bph resistance in the existing quality japonica cultivars is an important breeding target in Korea. Wide crosses using several strains of AA-genome wild rices, O.rufipogon have been used to produce the primary germplasm which is highly resistant to Bph. By repeated backcrossing, the resistance gene was introgressed in the background of two japonica cultivars, Ilpum and Hwaseong. Among the advanced backcross progenies, the ten BC2F3 lines were identified as the highest resistance in the comparative Bph bioassay with other resistant sources. The 24 polymorphic markers spanning the twelve chromosomes were tested for association with marker genotypes and resistance/susceptibility reaction in the 80 BC2F3 lines, RM3748 on the chromosome 7 showed the highest association.

Drought and salinity are two major environmental factors determining plant productivity that due to their high magnitude of impact and wide distribution. The regulatory circuits include stress sensors, signaling pathways comprising a network of protein-protein reactions, transcription factors and promoters, and finally the output proteins or metabolites. Plant receptor-like kinases (RLKs) are transmembrane proteins family, are predicted to be major components of the signaling pathways that allow plants to respond to diverse environmental and development condition. Subfamily of Catharanthus roseus RLK1-like kinases (CrRLK1Ls) is a novel type of RLK, was identified in Arabidopsis with 17 members carrying a putative extracellular carbonhydrate-binding malectin-like domain. To study the function of CrRLK1Ls subfamily in rice which is a most widely consumed staple food, we produced the phylogenomic data with the integration of microarray-based anatomical and stress expression profiling data to the context of rice CrRLK1Ls family phylogenic tree. The expression profiling data are based on a large number of public microarray data such as 1150 Affymetrix arrays and 209 Agilent 44K arrays. Chromosomal localization of CrRLK1Ls reveals that three of 16 genes were tandem duplicated. Subsequently, we identified 7 genes that showed circadian regulation pattern and three genes of them simultaneously response to drought stress: two were downregulated and one was up-regulated. Functional gene network development mediated by these stress responsible genes might be an useful foundation to explain the molecular mechanism of stress response mediated by this gene family.

Drought and salinity are the major abiotic stresses which are being continued to hamper the ecosystem and agriculture of the affected region. Plant species have adaptations to enhance their ability to tolerate stresses through physiological adjustment. Therefore, substantial amount of research are ongoing to provide insights about those mechanisms which enlighten the stress tolerance in plant. In this study, several rice cultivars were collected from the different parts of the world and ion accumulation experiments were conducted to select the best stress tolerant cultivar in drought and salt stress environment. For stress treatment, five rice cultivars were subjected to salt (200 mM NaCl) and drought (200 mM Mannitol) for 72h. Later Na+, Ca++, K+ concentrations in shoot and root samples were examined at different time interval. In both drought and salt stress, rice cultivar C201 (collected for uzbekistan) showed the lowest levels of Na+ ion and Na+: K+ ratio compared to other cultivars. It was significant parallel observation with pokkali (known salt tolerant cultivar). In this preliminary study, it was observed the C201 had more stress tolerant in terms of ion accumulation; however detail physiological studies are required to strengthen the idea regarding the best stress tolerant physiotype.

‘Jungmo2503’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2011. It was derived from the cross between ‘Dongsanpi81’ and ‘Kangbori’. Among the cross made in 1999, a promising line, SB992028-B-B-B-B-B-2, showed good characteristics in potential forage yield in the yield Trial tested at Iksan in 2007 to 2008 designated as Iksan 449. The line in the Regional Yield Trials (RYT) tested in eight locations around Korea for three years from 2009 to 2011, and was released as the name of ‘Jungmo2503’. It has the growth habit of group Ⅰ, erect plant type, green leaf and hood spike. Its average heading and maturing dates were on May 2, and May 29, respectively, with are similar to check cultivar ‘Yuyeon’. The cultivar had 102cm of culm length, 691 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Jungmo2503’ was about 10.9 ton ha-1 in dry matter in paddy field. ‘Jungmo2503’ also showed 9.4% of crude protein content, 27.3% of ADF (Acid Detergent Fiber), 49.0% of NDF (Neutral Detergent Fiber), and 67.3% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley.

High grain yield is one of the most important traits for improvement in rice breeding program. Much attention has been given to the genetic bases of spikelets per panicle (SPP) and grain weight (GW) because of their imporftance in rice yield. In this study, IL28, near isogenic line (NIL) developed by introgressing chromosomal segments from Moroberekan into Ilpumbyeo, showed significant increase in number of spikelets per panicle and 1,000 grain weight compare to the recurrent parent Ilpumbyeo. Quantitative trait locus (QTL) analysis in 1150 F2 plants derived from a cross between IL28 and Ilpumbyeo, indicated that both qspp6 and qgw6 were located in the interval RM3430 – RM20580. To map the QTL more precisely, substitution mapping of qspp6 and qgw6 using F4 lines was conducted. As a result of substitution mapping with fifty F4 lines, qspp6 was mapped to an 429kb interval between RM20521 and RM20562 while qgw6 was mapped to a 267kb interval between RM20562 and RM20572 based on the japonica genome sequence. This result seems to indicate that qspp6 and qgw6 are two different genes. It is notable that these QTL, qspp6 and qgw6 are independence from undesirable height and flowering time. Moreover, there was no negative correlation between qspp6 and qgw6 when two genes are pyramided in the genetic background of Ilpumbyeo. SSR markers tightly linked to the qspp6 and qgw6 will facilitate cloning of the gene underlying these QTLs as well as marker assisted selection for variation in SPP and TGW in an applied breeding program.

This study was conducted to investigate plant body temperature response of soybean (Glycine max) to saline stress. Two-weeks-old seedlings of soybean in V1 growth stage were treated with 0, 10, 20, 40, 80 and 160 mM of NaCl for salt stress. Thermal images acquired using Flir T-420 (US) were obtained at 4 days after treatment. Soybean leaf temperature increased with increasing NaCl concentration, resulting in significant positive correlation between soybean leaf temperature and stress intensity (P < 0.01). Leaf temperature of soybean was significantly different at 160 mM of NaCl, where no visual symptom was observed. Therefore, soybean leaf temperature can be used for evaluating the response of soybean to salt stress as a non-destructive and phenomic parameter. Non-destructive diagnosis of soybean leaf temperature may be a key parameter in a high throughput screening (HTS) system in breeding program for salt stress tolerance soybean cultivars.

UDP-glucose 4-epimerase catalyzes the reversible conversion of UDP-glucose to UDP-galactose. The gene, named BrUGE1, isolated from a Chinese cabbage composes of a total length of 1,328 bp that contains a single open reading frame (ORF) of 1,056 bp which encodes a polypeptide of 351 amino acid residues with a calculated mass of 39.0 kDa. Expression analysis showed that BrUGE1 is tissue specific and highly expressed in stem of rice plant. Interestingly, BrUGE1 mRNA was highly accumulated by drought stress with significantly higher amount of soluble sugar. Morphological evaluation showed an increase in yield and yield components compared to the wild type. Moreover, a better growth performance on galactose as well as higher UGE1 expression was observed in transgenic rice lines than in wild type. In the Ubi-1::BrUGE1 lines, the increase of UGE1 expression was apparently sufficient to overcome the toxic effects of galactose. Taken together, the Ubi-1::BrGUE1 rice lines increased yield probably by increasing the rate of filled grains. The enhanced drought tolerance may be due to the induction of soluble sugar which may act as osmolyte to compensate dehydration during drought stress.

Recently whole genome SNP genotyping has been used to do association analysis and to map a gene of interest. Here we report application of bulked segregant analysis(BSA) using Infinium HD assay with ‘BARC Bean6K_3’, a SNP genotyping beadchip containing 5,399 SNPs for common bean to locate a target gene. We used BSA using Infinium HD assay was performed to find the candidate region of a single dominant rust resistant gene in PI310762, a common bean cultivar. And SSR markers were identified and mapped on the candidate region using F2 population derived from the cross of susceptible Pinto114 x resistant PI310762. BSA revealed the candidate region of the resistant gene is on chromosome 4 where we developed nine SSR markers. Three SSR markers (beanssr1170, beanssr1168, and beanssr1167) of them appeared closely linked to the resistant gene which is located between beanssr1167 at 0.1cM and beanssr1170 at 0.5cM on chromosome 4. This study showed BSA using high-throughput whole genome SNP genotyping is a very fast and efficient method to locate a gene of interest on chromosome.

The aim of this study was to select the abiotic tolerant sorghum mutants using chlorophyll a transient OJIP analysis of PSⅠ and PSⅡ so called Kautsky’s effect within 1 second. It was clearly identified that wwt-and drought tolerant sorghum mutants could be classified by wet factor index(WFI). On the basis of WFI, wet tolerant sorghum matants were classified as follows; Ⅰ group, MUT534 bmr/new, MUT525 bmr; Ⅱ group, M2P1207 bmr, 25M2-0404 bmr, MUT371 bmr24, unknown bmr22, 10M2-0775 bmr, MUT135 bmr23; Ⅲ group, M2P0411 bmr, MUT641 bmr, M2P1064 bmr36, MUT855 bmr, 25M2-0137 bmr/new, MUT436 bmr, M2P0929 bmr, 25M2-0026 bmr, 10M2-0387 bmr, 25M2-0173 bmr/new; Ⅳ group, 25M2-0698 bmr. In conclusion, for the selection of wet tolerance, four photochemical parameters such as Electron transport flux until PSI acceptors per PSII(RE1o/RC), Performance index for energy conservation from photons absorbed by PSII antenna, until the reduction of PSI acceptors(PI_total ABS), Driving force on absorption basis(DF_total ABS) and Electron transport flux from QA to QB per PSII(ETo/RC) were important photochemical parameters deduced from maximum quantum yield and electron transport efficiency.

Grain size is a major determinant of grain yield in rice. In a previous study, a QTL for grain width(GW), qgw1 was detected on chromosome 1 using 96 BC3F8 lines derived from a cross between ‘Hwaseongbyeo’ as a recurrent parent and ‘O. rufipogon’ as a donor parent. At this locus, the O. rufipogon allele increased GW. Among the 96 introgression lines, three ILs with the O. rufipogon qgw1 locus showed significantly increase in grain width compare to the recurrent parent. One of the three lines, CR572 was selected and crossed to ‘Hwaseongbyeo’. A total of 494 F2:3 were evaluated for grain width and agronomic traits in the field. QTL analysis in 494 F2:3 lines indicated that QTL for grain width was located in the interval RM495–RM5443. To narrow down the position of qgw1, substitution mapping using F4 lines with different cross-over breakpoints in the region is underway. The result will be discussed.

This study aims to identify major quantitative trait loci (QTL) for yield components under low-input systems in tropical regions in rice. A total of 156 highly advanced recombinant inbred lines (RILs) have been developed from a cross between two temperate rice varieties, Dasanbyeo (Tongil-type indica) and TR22183 (japonica). Both parental lines and RILs were tested under two different regimes of irrigation and phosphorus (P) application levels. During the wet season of 2012, under mild drought conditions, TR22183 showed more vigorous root growth at 15 days after sowing than Dasanbyeo. The early root establishment of TR22183 may have contributed to the enhanced P uptake from the top soil in the early growth stage. The linkage map for DT-RILs was constructed with 312 single nucleotide polymorphism markers aided by 384-plex platform of BeadXpress high-throughput genotyping system. For the vegetative growth, major QTLs on chromsome 6 for plant height and tiller numbers were identified. For the grain yield related traits, major QTLs on chromsome 2 were closely linked to each other. On the other hand, panicle length QTLs were identified on chromsomes 2 and 9. We are currently analyzing phenotypic data and the experiment is being repeated in dry season and temperate region.

This study was conducted to provide basic data for high-throughput screening (HTS) system construction based on phenomics. Rice (Oryza sativa cv. Chucheongbyeo) seedlings in vegetative growth stage were grown in the glass house and treated with 0, 3.75, 7.5, 15, and 30% (w/v) of polyethylene glycol (PEG) to give osmotic stress. Three days after PEG treatment, hyper-spectral reflectance images were obtained and analyzed after removing background image in several steps. The reflectance of rice seedlings treated with 15 and 30% of PEG solutions were significantly different at 680 nm, where differences in the chlorophyll reflectance spectrum and visual symptoms were not observed. These results thus indicate that hyper-spectral reflectance observed at 680 nm can be used to screen drought tolerant rice lines. A HTS system equipped with this hyper-spectral reflectance system may play an important role of future rice breeding program.

In order to find new genetic sources of rice salt tolerance, we did screening with about 10,000 rice mutant lines created by Ac/Ds insertional mutagenesis. First, we raised rice seedlings with media soil on 0.7% NaCl solution and selected 71 putative salt tolerant lines and analyzed their Ds insertion sites. We tested their salt tolerance by growing seedlings on MS medium containing, 0 mM, 150 mM, and 250 mM NaCl. Also, their seedling salt tolerance were evaluated by growing on Yoshida nutritional solution containing 0.6% NaCl. Finally, we selected eight mutant lines showing increased seedling salt tolerance compared with wild type variety, Dongjin, repeatedly. We grow them in rice field and investigated their agronomic traits such as heading time, culm length, panicle length, and panicles per hill. Among them two lines which were named Salt10 and Salt23 and showed favorable agronomic characteristics were crossed with Dongjin for further genetic analysis and mapping the causative gene variation.

옥수수는 타식성 작물로 잡종강세 육종을 하기 위한 자식계통이 요구되며 육종효율을 높이기 위해서는 반드시 자식계통들의 특성 평가가 선행되어야 한다. 본 시험은 농촌진흥청 국립식량과학원에서 육성한 단옥수수 자식계통들의 특성을 평가하여 육종효율을 높이고 유전체 연구의 자원으로 활용코자 수행하였다. 시험에 사용된 계통들은 국립식량과학원에서 육성한 KSE1 등 sugary enhancer(se) 유전자를 가진 단옥수수 자식계통 45점이며 4월 중순에 국립식량과학원 밭작물시험포장(수원)에서 재식거리 60×25㎝(6,600본/10a)로 파종하여 발아율, 식물체특성, 생육특성, 화분 및 종자생산 관련 특성 등을 조사하였다. 발아율은 KSE2가 31%로 가장 취약하였고 수광태세와 관련된 엽신과 줄기사이의 각도는 21~65°로 다양하게 분포하였으며 숙기와 관련된 출사일수는 47~67일로 다양하게 나타났다. 화분의 생산량과 관련된 웅성소수의 밀도는 3~7, 1차 지경수는 5~25개, 웅수의 길이는 19.1~40.7cm 범위였다. 종자생산량과 관련된 이삭의 길이는 7.8~16.2cm, 폭은 3.0~4.5cm, 이삭당 열수는 10~18열 범위였다.

구기자나무는 열매, 잎, 뿌리 등 이용부위가 다양한데 지표성분인 베타인이 구기순과 잎에도 존재한다. 중국에서는 열매가 달리지 않고 줄기만 자라는 Ningqi vegetable No. 1을 육성하였고, 각종 요리에 상용하고 있다. 따라서 구기순을 대량생산하기 위해서는 맹아력이 왕성하고 순이 굵으면서 잎이 넓은 계통을 선발할 필요가 있다. 이를 위해 청양구기자시험장에서 보존하고 있는 구기자나무 유전자원 143계통에 대하여 특성검정을 실시하였다. 구기순은 맹아되는 새순을 10cm 길이로 채취하여 분석하였다. 구기순의 엽형지수는 0.37 ± 0.068로 피침형이었고, 베타인 함량은 평균 2.8 ± 0.89%이었으며, 대한약전에서 규정한 0.5%(열매)보다 높았다. 잎 폭은 길이나 두께에 비하여 변이계수가 높았고, 구기순의 건조 무게는 줄기 굵기와 정의 상관관계를 나타내었다. 5월 2일에 채취한 구기순의 베타인 함량과 8월 24일에 채취한 잎의 베타인 함량을 분석한 결과 두시기 모두 베타인 함량이 높은 계통으로 IT232687, 232711, 232648 등 3계통을 선발하였다. 또한 두시기의 베타인 함량에 대한 단순상관을 분석한 결과 정의 상관관계를 나타내었고, 생육초기에 베타인 함량을 분석하여 그 함량이 낮은 계통을 조기에 도태하는 것으로 육종효율을 높일 수 있을 것으로 판단되었다. 구기순의 건물중이 높고 베타인 함량이 높은 계통으로 IT232589, 232648, 232676, 232679, 232705등 5계통을 선발하였고, 앞으로 구기순 생산 전용품종을 육성하기 위한 육종소재로 이용할 수 있을 것으로 사료되었다.

국내 적응 Quality Protein Maize (QPM) 옥수수 교잡종을 육성하려면 효율적인 선발 체계를 확립하는 것이 필요하다. 본 연구는 최근 국립식량과학원에서 육성중인 QPM1 등 4 교잡계 종실의 일반성분, 지방산 조성 및 아미노산 함량을 대비품종인 일반 옥수수 장다옥과 비교 검토하여 QPM 옥수수 신품종 육성을 위한 기초 연구 자료로 활용하고자 실시하였다. QPM 교잡계들의 단백질함량은 10.0〜11.40%로 장다옥의 8.1%보다 모두 많았다. QPM 교잡계들의 지방함량은 3.8〜4.0%이었으며 QPM1을 제외한 QPM 교잡계들은 장다옥과 차이가 없었다. QPM2 교잡계를 제외한 QPM 교잡계들의 회분함량은 장다옥보다 많았다. QPM 교잡계들과 장다옥 모두 linoleic acid의 조성비가 가장 높고 oleic acid, palmitic acid, stearic acid, linolenic acid의 순으로 지방산 조성비가 높은 것으로 나타났다. QPM 교잡계들의 포화지방산 함량은 17.2〜18.4%로 장다옥과 비슷하였다. QPM 교잡계들의 불포화지방산 함량은 81.6〜82.8%로 장다옥과 비슷하였다. QPM 교잡계들과 장다옥간 필수아미노산 중 isoleucine, valine, threonine, 황을 함유한 methionine, cystine 등의 함량 차이는 없었으나, lysine함량은 QPM 교잡계들이 장다옥(3.84g/100g)보다 4.05〜4.69g/100g로 많았으며 그중 QPM1 교잡계가 4.69g/100g으로 가장 많았다. QPM 교잡계들과 장다옥간 산성아미노산인 MMA(monoamino monocarboxylic acid), DMA(diamino monocarboxylic acid), 방향족 아미노산인 AAA(aromatic amino acid) 등의 함량 차이는 없었다. 본 연구에서 육성된 국내 적응 QPM1 교잡계는 옥수수의 경우 필수아미노산 중 가장 문제시 되고 있는 lysine 함량이 보통 옥수수보다 개선되어 식용 및 사료용 옥수수로 활용 가치가 높을 것으로 기대된다.

“장새미” 품종은 제주특별자치도농업기술원에서 수집한 유전자원 중 조숙 다수성 계통을 육성하기 위하여 자체 선발하고 있는 유전자원 중 중국 산동지역에서 수집한 모집단 제주-도입-501호 모집단에서 계통분리에 의해 1998년 선발하였고, 그 중 2차 생장 등 환경변이를 일으키는 개체를 제외하고 인편분화가 빠르거나 화경 및 주아가 이상적으로 비대한 개체를 중심으로 2003년 계통분리 육성하였다. 2003년부터 2005년까지 생산력 검정, 2006년부터 2009년까지 지역적응을 거쳐 “장새미”로 명명하였다. 2010년 품종보호 출원하여 2012년 품종보호등록 되었다. “장새미” 품종의 숙기는 조생종이며 생육적온은 중온이다. 생리적 특성은 잎색은 연녹색이며 인편분화기 1월 중·하순, 화경추대는 완전추대 형이고 구색은 진한 자색을 띤다. 재배적 특성은 제주지역에서 겨울철 지상부 생육이 왕성하게 진행되고 화경을 제거하면 구 비대가 급속히 진행되고 잎 노화도 빠르게 진행된다. 자식체 특성은 화경이 직립형태에서 돼지꼬리 모양과 성숙이 진행되면 직립으로 진행되며 총포내 주아수가 많고 1개 주아무게가 0.2g이하이다. 구 모양은 넓은 편원형이며 저반의 위치는 편편형이고 알리인 13.070mg/kg, 알리신 함량이 189mg/kg이다. 평균 구중은 52.5g이고, 초장은 85㎝, 평균 엽수는 9.1개, 초형은 반개장형이다. 마늘의 수확 시기는 제주지역에서 4월 하순부터 5월 중순까지 수확이 가능하다. 재배상의 주의점은 질소비료를 과용했을 때 병 발생이 많고 특히 무름병이 증가할 수 있으므로 균형시비를 해야 한다. 또한 조숙성이기 때문에 다른 난지형 품종보다 빠른 비배관리가 필요하다.

거베라 ‘Pinkholic’은 2007년 수원 국립원예특작과학원에서 자주색 반겹꽃 ’A Cappella’와 분홍색 반겹꽃 ‘Peter’를 교배하여 획득한 종자로부터, 2007년 실생 계통을 양성하여, 화색이 선명하고 화형이 안정된 대륜계통의 분홍색 반겹꽃 거베라 ‘07B3-2’를 개체 선발하였다. 선발된 계통에 대하여 2008년부터 2011년까지 개체 증식 및 1·2차 생육특성검정을 수행하고, 2012년에 ‘원교B3-49호’로 계통명을 부여하여 3차 특성검정, 안정성·균일성에 대한 연차별 재현성 및 기호도 평가를 수행한 결과, 화색 및 화형에 대한 기호도가 우수하고, 절화수명이 우수한 품종으로 그 우수성이 인정되어, ‘원교B3-49호’는 2012년 농촌진흥청 직무육성품종심의회를 거쳐 ‘Pinkholic’으로 명명되고 직무육성품종으로 등록되었다. ‘Pinkholic’의 생육 및 개화특성은 화색과 화형이 유사하며 교배모본으로 사용된 ‘Eta’를 대조품종으로 하여 조사하였다. ‘Pinkholic’은 녹색 화심의 RHS color chart RP58C의 분홍색 반겹꽃으로, 대조품종(RHS R52C)보다 밝은 분홍색 품종이다. 평균 화경은 12.0cm로 대조품종보다 0.8cm 큰 대륜계 품종이며, 설상화의 길이와 폭도 각각 5.7cm와 1.2cm로 비교적 길고 넓다. 대조품종의 꽃대 굵기가 꽃목부위 4.0mm, 중간부위 5.5mm인데 반해, ‘Pinkholic’은 각각 4.9mm, 6.8mm로 비교적 굵고 둥글어 경할현상이나 꽃목굽음 등의 생리장해 발생이 적고, 절화수명도 평균 10.4일로 길다. 절화장이 평균 55.7cm 정도로 길어 절화 품질이 우수하며, 연간 채화량도 53.9(본/주) 정도로 많은 다수성 품종이다.