Although canine brucellosis has been known to be an important re-emerging zoonosis, the pathophysiological mechanisms of Brucella canis infection remains clues to be solved. Different culture models, single and co-culture models, were constructed with canine epithelial cells, D17 and macrophage, DH82 to investigate the induction of immune responses in in vivo B. canis infection. Expression of genes related with induction of immune responses, Th1, Th2 and Th17, was compared in the two different models after the bacterial infection. In this study, expression of cytokine genes, IL-1β, IL-5, IL-6, IL-10, IL-23, and TNF-α was quantified in the DH82 at different time points using RT-qPCR in the two different culture systems after the infection. Cytokine genes related with Th1, IL-1β and TNF-α and Th17, IL-6 and IL-23 were expressed with time-dependent manners in the both systems (p<0.05). However, increase of Th2-related cytokine genes expression was not detectable in the both systems by comparison with control. The expression of Th1 and Th17 related cytokine genes was earlier in single cell culture than those in co-culture model (p<0.05). In general, amounts of the expressed genes were shown higher in single cell model than those in co-culture models. This study indicate that Th1 and Th17-associated immune responses are central to B. canis infection in dogs. In addition, it suggests a specific role of epithelial cells in the B. canis infection in vivo, which should resolved in the further study.

최근 몇 년간 국제 테러단체가 점점 확대고 있으며, 파키스탄은 새로운 테러단체인 “Arch of Instability” 의 중심이 되고 있다. 이는 파키스탄의 국내 정치 경제 발전에 심각한 위협을 줄 뿐만 아니라 국제 안보 구조와 대테러 국제정세에 심각한 위협을 가하고 있다. 또한 이는 중국 “일대일로”의 중요한 프로젝트인 “파키스탄 경제 통로”에도 심각한 위협이 되고 있다. 따라서 기존문헌을 정리한 결과를 파키스탄의 테러 상황에 대한 추가적인 연구가 필요하여 본 논문은 컴퓨터 과학 및 기술영역에서 새로 부각되고 있는 시각화 연구방법을 사용하여 Web of Science 데이터베이스를 활용하고 국제 학술계가 파키스탄의 테러에 대한 연구결과를 시각화 분석하여 국제 학술계가 연구한 파키스탄 테러단체의 국가간의 협력망도 제출하였다. 더불어 파키스탄 테러 연구에 관한 국제 학술계의 저자 및 협력 네트워크에 대한 양적인 분석으로 파키스탄 테러 연구소의 국제 학술계의 연구 핫스팟 및 연구 트렌드도 정리하였다.

At present, ECDIS has been widely used in ship positioning, collision avoidance and navigation, and has developed into an important part of integrated navigation and automation system. However, most ECDIS software focuses too much on system function and technical level when realizing powerful navigation AIDS, neglecting the problems that the crew are most concerned about, such as realizing the human-computer interface operation quickly and simply as well as obtaining the information of human-computer interaction effectively and accurately. In order to improve the design of Electronic Chart Display and Information system（ECDIS）human-computer interface and its usability effectively, a new method for evaluating ECDIS human-computer interface is proposed. Starting from the concept and connotation of usability, five-factor personality model is used to measure the users’ personality type in reference to the essence of usability (user’s emotional experience) and calculate the basic emotional variant of users. Then, according to the users’ emotional stimulation after finishing the given ECDIS task and combining with the system Usability scale (SUS), a tool dedicated to usability testing and five dimensions of usability evaluation proposed by Jakob Nielsen, father of usability, the emotion relationship model of usability and users is established by using partial least square method ( PLS ), and the validity of the model is verified with an aim to locate the key indicators that affect the usability of the ECDIS human-computer interface, It will be more helpful to improve the usability of ECDIS human-computer interface.

The multilateral trade relations between Malaysia and MERCOSUR is a new market. This study focuses on automotive policy areas towards the MERCOSUR regime which focus on trade ties. The purpose of this study is to expand national automotive policy to penetrate the automotive sector in the MERCOSUR. While it create opportunities to penetrate the automotive sector in MERCOSUR where Malaysia needs to enhancement national trade performance of FTA potential within MERCOSUR regime especially on investment and trade. The main issue is the lack of progress and process are restrictions on Malaysia's within MERCOSUR in the automotive field. Malaysia does not pursue trade within respective nation but in statistical shows have a large and positive market economy with the southern continent of America, especially the automotive industry and lack of performance of Malaysian counterparts and MERCOSUR and unsuccessful automotive investment and exporters. This research uses qualitative research methods, data collection, interviews, and content analysis methods for this study. Expert from respective agencies as interviewees such as the Ministry of Foreign Affairs (MOFA), Malaysia Trade Relations Division (MATRADE), the Department of Robotics and the automotive trade policy of Malaysia and the IoT Institute. Coding techniques are used for coding processes in content analysis. Hereby, the analysis by using the Economic Integration theory with SWOT Analysis to find out the implementation of Malaysia FTA within the MERCOSUR .Hence, research findings show that Malaysia's multilateral within MERCOSUR relations have a potential to be strengthened with a variety of positive and initiatives from the Malaysia government.

The shipping revolution was introduced due to development and improvement that occurred in the shipping industry. Until now, changes in the shipping industry are still ongoing to improve their services, especially by introducing mega ships to transport cargoes. Thence, the shipping revolution stimulates seaport to comply with the requirements for a new mega ship. Therefore, this study was conducted to determine the external environment factors that contribute to the challenge of Malaysia Port towards the shipping revolution. Firstly, the external environmental factors that challenge Malaysia Port towards shipping revolution are identified by using the PESTEL analysis; which are political, economic, social, technological, environmental, and legal factors. Then, these identified factors are ranked by using Analytic Hierarchy Process (AHP) to determine the priority external environment factors that contribute to the challenge of Malaysia Port towards shipping revolution. Data were collected by using pair wise comparison questionnaire which is qualitative method. The questionnaire consists two sections; there are demographic information and PESTEL factors. Thereafter, the data were analysed by AHP analysis. The results shown that the top three priority external environmental main factors that contribute to the challenge of Malaysia Port towards shipping revolution which are economic, technology and environment. Then, the top three sub factors are economic growth, digitalization and reduce emission. As a conclusion, these factors can influence the Malaysia Port towards shipping revolution and need priorities attention. Whereas the economic growth will monetary support shipping and port revolution, digitalization can reduce cost and increase efficiency, and reduce emission towards environmental friendly. Consequently, the findings will raising awareness and as a blueprint to government and port operators during development and expansion of harbour towards shipping revolution.

The present work introduces a new method for the recycling of waste flocculation sludge to prepare electrode materials for supercapacitor. Hazardous azo dye was removal from textile dying wastewater by a new chitosan-based flocculant, and the generated dye sludge flocs was used as a nitrogen-containing precursor for the fabrication of N-doped carbon materials. The influence of azo dye on specific surface areas, nitrogen content, pore evolution of the resulting products and their electrochemical performance were investigated in detail. The results demonstrated a dual role of azo dye worked as both a nitrogen resource and pore-forming agent. The resulting N-doped carbon nanosheets derived from azo dye flocs demonstrated high electrochemical capacitance and good stability for supercapacitor electrode, which is attributed to the unique nitrogen doping, higher specific surface area and efficient charge transfer ability.