This study was performed to establish the control strategies effectively using Eco-friendly agricultural materials (EFAM) and natural enemy when cultivating autumn-type leaf perilla and to investigate the population densities of seasonal major pests such as Tetranychus urticae (two-spotted spider mite, TSSM), Broad mite (Polyphagotarsonemus latus), Aphis egomae, and Pyrausta panopealis. TSSM showed the occurrence in the seedling stage from August to the end of September and controlled using EFAM in the nursery. Broad mites had a low occurrence in October, December, and the following year showed the density of two or more leaves per leaf from February. Aphis egomae was locally prevented around the area of occurrence, and it showed more than five per leaf in October. Pyrausta panopealis seemed to occur from August to September. Aphis egomae is control using high-toxic EFAM before the overwintering and prevents it from using banker plants planted barley in the winter. Pyrausta panopealis is protected by biological pesticides in August registered in the leaf perilla.

시설재배 딸기에서 작은뿌리파리의 친환경 방제법 개발을 위하여 2년간 수행하였다. 피복재로 배지를 피복하고, 고리형 점착트랩을 설치하면 효과적이었다. 피복재 종류별 작은뿌리파리 발생정도는 무피복에서 트랩 당 67.1마리로 가장 많았고, 흑백필름 등 나머지 피복재에서는 2～5마리로 낮은 밀도를 유지하였다. 피복재 종류별 딸기 생육특성에서 초장, 엽장, 엽폭은 처리간에 비슷한 경향이었으나, 화방수는 무피복에서 9.7개로 가장 적었고, 수량은 적색필름과 흑백필름 피복이 무피복에 비해 14.6～9.2% 증수하였다. 하절기 피복재 종류별 근권 온도는 흑백필름과 적색필름이 주야간 편차가 적고 안정적이었으며, 동절기에도 적색필름은 다른 피복재보다 근권 온도가 높고 안정적이었다. 점착트랩 색상별 작은뿌리파리 유살정도는 큰 차이가 없었고, 트랩 형태별에서는 고리형이 원반형보다 다소 많았고, 설치 노력이 57.1% 절감되었다.

매실에서 복숭아씨살이좀벌에 대한 친환경 방제법 개발을 위해 2년 동안 수행하였다. 낙과된 매실 핵을 수거하여 보관 장소를 달리하였는데, 장마전에 수거해서 노지에 방치한 경우는 10.7%가 월동하여 모두 성충이 되었다. 수거한 매실 핵을 온실에 보관하다 10월에 노지에 방치한 경우에는 23.3%가 월동하였고, 이 중 77.1%가 성충이 되었다. 그러나 수거 당시부터 이듬해 3월까지 온실에 보관한 경우는 월동률이 42.4%로 매우 높았지만 단지 3.1%만이 우화에 성공하였다. 피해 매실 핵에서 발생한 곰팡이균 Fusarium sp., Geotrichum candidum 2종을 동정하였는데, 매실 핵이 빨리 부식된 경우에는 유충이 핵내에서 곰팡이에 감염되거나 물이 스며들어 사망하였다. 곰팡이 종류는 Fusarium sp.이 더 효과적이 었고, 피복작물로 헤어리베치를 재배하면 더욱 부식이 빨라 핵의 무게와 경도가 낮아져 유충의 월동을 제한하였다. 한편 품종별 피해과율은 옥영품종이 88%로 가장 높았고, 천매품종은 피해가 없어 단일품종보다는 여러 품종을 섞어 재배하는 것이 필요하였다.

For economic effect on an import prohibition for the buff-tailed bumblebee, income of the bumblebee products, function, economic effect of pollinating insects and bumblebee were analyzed. The largest incremental gain was observed when using domestic bumblebees. Incremental revenue showed a larger incremental revenue in pollination than artificial pollination, the cost of artificial pollination was lowest for incremental cost, followed by domestic bumblebees, honeybees and imported bumblebees. Consequently, higher economic value can be achieved when domestic bumblebees and honeybees are used. According to this study, as it is used to make policy decisions on the prohibition of import of buff-tailed bumblebee, other alternative plan can be utilized due to its import prohibition.

Stathmooda masinissa is one of the most serious lepidopteran pests which damaging the furit of persimmon. Its pheromone composition has been idetified as (E4,Z6)-4,6-hexadecadienyl acetate (E4,Z6-16Ac) and (E4,Z6)-4,6-hexadecadien-1-ol (E4,Z6-16OH). We evaluated electrophysiological response of (E4,Z6)-4,6-hexadecadienal (E4,Z6-16Ald) and attractivenss of E4,Z6-16Ald by combination of two pheromone components. E4,Z6-16Ald evolked electrophysiological responses as same to that of two pheromones. The lure combined with E4,Z6-16Ald and two pheromone components attracted more male S. masiissa.

Pine wilt disease (PWD) induced by the pinewood nematode (PWN), Bursaphelenchus xylophilus, is a great threat to pine forests in Europe and East Asia. Identification of interrelation between Monochamus spp., the major vectors of PWD and PWN is the key factor for effective control of PWD because PWN is trasmitted to healthy pine trees only by Monochamus vectors. In this study, direct injection system is designed to apply PWN artificially to M. alternatus just before emergence to adults. After injection of PWN (300~500 nematodes in 10 μl of 1X PBS) using adapted glass pipets, the survival rate of M. alternatus and detection rate of live PWN were 80.0% and 68.8% at 168 h respectively. As a result, we demonstrated the artificial injection methods of nematode to Monochamus vectors for further study of coeffect between vectors and specific stage of PWN including 3rd stage dispersal juvenile.

Pine wood nematode (PWN), Bursaphelenchus xylophilus causes devastating damage to pines worldwide. To develop new agent for controlling PWN, alkyloxyalcohols(A-H) were synthesized and nematicidal activity was tested against PWN. As a control, monochamol, which reported to have nematicidal activity was also tested. The nematicidal activity was diffferent according to carbon chain length in compounds. LC50 values of F, G and H were as same as that of monochamol. However A-E which have shorter carbon length than F-H showed weak nematicidal activity.

전국 136개 시군에 있는 690개소 벼 관찰포에서 12종의 주요 해충을 6월 1일부터 9월 13일까지 15일 간격으로 총 8회 조사한 결과를 국가농작물병해충관리시스템(NCPMS)을 통하여 수집하였다. 2017년도 벼 주요해충 12종의 총 발생 추정면적은 158,942ha로 재배면적 대비 21.1%에 발생되었다. 발생면적이 가장 많았던 해충은 애멸구로 43,875ha(재배면적 대비 5.8%)이였고, 그 다음으로는 혹명나방 34,112ha(재배면적 대비 4.5%), 벼물바구미 22,802ha(재 배면적 대비 3.0%), 먹노린재 21,118ha(재배면적 대비 2.8%)순 이었다. 평년보다 발생면적이 많았던 해충은 애멸구, 멸강나방, 먹노린재로 애멸구는 평년보다 113%수준, 멸강나방은 평년대비 136% 수준, 먹노린재는 269% 수준이었다. 먹노린재의 발생이 점차 증가하고 있는 것을 확인할 수 있었다. 결론적으로 2017년 벼 주요해충의 발생정도는 전년 대비 96% 수준으로 유사하였지만, 평년대비는 56% 수준으로 발생이 낮은 경향임을 확인하였다.

Carbonyl sulfide is a chemical that is registered as a crop protection product in Australia due to microorganisms such as soil, plant roots, branches, fertilizers and compost. In order to develop new fumigants for domestic use, the susceptibility of Carbonyl sulfide fumigants to two agricultural pests was evaluated. The susceptibility of peach aphid and spotted mite, which is an agricultural pest, was evaluated. After 5 hours of treatment (10, 20, 40, 60, and 80 mg / L) . As a result, peach aphid larvae showed a high mortality rate of 97.6% at 10 mg / L and 100% at 20 mg / L or more. Larvae were found to have a rate of 95.8% at 80 mg / L. The insect mortality rate was 91.8% at 60 mg / L and 94.1% at 80 mg / L.

Mediterranean fruit fly, Ceratitis capitata, is one of the most important quarantine pest worldwide. Fruit flies cause serious damage on orchard, and also can cause quarantine problem to fruit exportation. Recently, because of global warming, there is threats of fruit fly invasion to Korea, especially to Jeju island. So there should be a anticipative research about fruit fly control. Many physical quarantine treatment methods, like heat treatment and low-temperature treatment, are developed to control quarantine pests. Recent researches indicate that low-temperature treatment shows good efficacy on several quarantine pests without phytotoxicity. In this trial, we’ve applied the low-temperature treatment on citrus at laboratory and pilot scale containers to validate efficacy and phytotoxicity. Egg and larvae stage of C. capitata was completely controlled after 5 days and 8 days low-temperature treatment at 0℃ respectively, and there was no phytotoxicity on citrus. These results indicate that the low-temperature treatment can be applied to control fruit fly on citrus fruit.

The susceptibility of the Cigarette beetle, which are pests in tobacco, to methyl bromide and phosphine fumigants was evaluated. Five concentrations of each methyl bromide were selected for all stages and treated for 4 hours. As a result, 100% larvae were observed in eggs CT 51.20, late larva CT 73.61, pupa CT 71.87 and adult 52.87 mg h / L, respectively. The LCT50 values for methyl bromide were 13.896 for eggs, 36.038 for late larvae, 25.172 and 21.758 mg / l, respectively. The phosphine treatment was carried out for 5 to 6 concentrations (0.025, 0.051, 0.099, 0.501, 0.999 and 1.500 mg / L) for 20 hours. As a result, egg and late larvae showed 100% larvae at CT 5.137 and 6.435 mg h / L, respectively, and 61.9% and 98.9% of larvae and adult larvae were similar to each other. Pupae showed 86.4% larvae at CT 10.520 and 100% larvae at over 13.777 mg / h of CT. The LCT50 values for phosphine were 0.317 for eggs, 0.649 for late larvae, 3.748 for pupa and 0.703 mg / l for adults.

Riptortus pedestris is a polyphagous species and an economically important insect pest of soybean. We investigated the phenology of R. pedestris at 13 locations - Jeonju, Naju, Goheung, Milyang, Gunwi, Andong and 7 locations of Jeju. Fish trap was used for monitoring R. pedestris. The traps were baited with lures containing a 1:5:1 ratio of myristyl isobutyrate, (E)-2-hexenyl (E)-2 hexenoate and (E)-2-hexenyl (Z)-3-hexenoate. There was difference the first adult catch and local temperatures among sampling sites. To determine spring emergence distribution and annual phenology pattern, we used the monitoring data and ambient temperature from each sampling sites. We compare the seasonal occurrence and spring emergence between 2016 and 2017 in each location.

Riptortus pedestris is one of economically important insect pests in soybean fields. The study was conducted at six constant temperatures of 15.8, 19.7, 24.0, 27.8, 32.6 and 35.5℃. We investigated the biological traits of R. pedestris adults. R. pedestris adults survived at all temperatures tested but female did not oviposit under 15.8℃. The longevity of R. pedestris male was longer than that of female. Oviposition model was constructed using nonlinear functions and the number of eggs produced by a cohort of females was estimated in relation to constant temperature and adult age. The life table parameters in relation to temperature were analyzed.

The oriental fruit fly is a major polyphagous insect pest with a worldwide distribution. The effects of temperature on stage-specific development were investigated at eight constant temperatures (13.0, 14.4, 16.2, 19.5, 23.8, 27.7, 31.8 and 34.8℃). Stage-specific lower developmental thresholds and thermal constants were determined using linear regression. The lower and higher temperature threshold (TL and TH) were estimated using the Sharpe-Schoolfield-Ikemoto (SSI) model. The daily adult emergence frequency of B. dorsalis was estimated in relation to adult age and temperature. Thermal performance was compared among B. dorsalis populations from different locations in Taiwan.

The oriental fruit fly, Bactrocera dorsalis (Hendel), has potential of serious damage to various vegetables or fruits, especially genus of Citrus, such as mandarin orange and tangerine in Korea, where its larvae do damages. Animal and Plant Quarantin Agency of Korea and Animsl Systematics Laboratory of Kunsans National University have collected the samples of oriental fruit fly in East Asian countries, Vietnam, Philippines, Myanmar, China, Cambodia, India Nepal and Laos. To confirm genetic differences and structure of B. dorsalis population samples collected from those countries, we analyzed 192 individuals from 28 locations over 7 countries using 15 microsatellite loci. In total samples, number of different alleles, number of effective alleles and Shannon's Information Index were 6.421±0.364 (standard error, SE), 3.664±0.137 (SE) and 0.902±0.030 (SE), respectively. Grand means (±SE) of observed and expected heterozygosity over all loci and populations were 0.534 (±0.018) and 0.597 (±0.017), respectively. Among all populations, Fst values ranged from 0.016 to 0.705 with averaging 0.194 (±0.026).

본 조사는 채집방법에 따른 곤충군집을 비교하기 위해 공검지 습지보호지역 내에서 쓸어잡기, 함정트랩, 버킷트랩 세 가지 방법을 이용하였다. 조사 시기는 5월 15~16일, 8월 3~4일, 11월 6~7일 총 3회 실시하였으며, 조사는 보호지역 중심부에서 실시하였다. 본 연구결과 세 방법으로 총 7목 51과 110속 120종 14,717개체가 채집되었다. 채집방법별로 쓸어잡기에서 4목 25과 36속 37종 331개체, 함정트랩에서 3목 12과 18속 19종 30개체, 버킷트랩에서 6목, 38과 76속, 84종, 14,121개체가 조사되었다. 군집 분석결과 다양도 지수는 쓸어잡기가 3.63으로 가장 높게 나타났으며 풍부도 지수는 버킷트랩이 8.69로 가장 높게 나타났다. 유사도 지수는 세 방법이 서로 0.2이하로 매우 낮았다.

참나무와 서어나무는 온대지역에 걸쳐 넓게 분포하고 있으며, 다양한 초식곤충의 먹이원이 되는 것으로 알려져 있다. 이 연구에서는 지리산국립공원 온대림에서 참나무림(반선)과 서어나무림(상선암)에서 초식곤충 활동량을 알고자 하였다. 각 조사지역에 0.1ha의 방형구를 설치하여 참나무와 서어나무의 분포를 확인 한 뒤 개체별로 초식곤충의 활동량인 잎 손상 지수를 측정하였다. 잎 손상 지수는 손상 퍼센트로 환산하여 분석하였다. 측정결과 전체 4,413장의 잎을 관찰하였으며, 참나무림에서는 2,683장, 서어나무림에서는 1,730장의 잎이 조사되었다. 초식곤충의 활동량은 참나무림과 서어나무림에서 각각 차이를 보였으며 서어나무림인 상선암에서 참나무의 식흔량과 서어나무의 식흔량 이 참나무림인 반선보다 모두 높게 나타났다. 이 연구결과를 바탕으로 온대림 우점 수종인 참나무와 서어나무에서 초식곤충의 활동량을 정량적으로 추정할 수 있을 것으로 생각된다.

One of the leading pests of rice, small brown planthopper (Laodelphax striatellus) can grow up to have either short or long wings, depending on conditions. However, under the same breeding conditions, the phenotypes of the long- and short-winged small brown planthopper observed to keep the first collected phenotype. To investigate the mechanism involved in wing dimorphism, metabolomic researches have been conducted. In this study, we observed several metabolites change, and the difference of metabolites could provide clues to the relationship between physiological changes in the small brown planthopper and ecological transport.

Mosquitoes are transmit many dangerous disease such as malaria, yellow fever and dengue fever. So far, chemical insecticides such as DEET have been mainly used to control mosquitoes, but there are many side effects. This study used ultrasonic sounds as an alternative to chemical insecticides. We found that Culex pipiens, which are common in Korea, exhibit avoidance behavior in a specific ultrasonic frequency. Through electrophysiological recording, we have inferred that avoidance behavior is caused by different from each other mechanisms depending on the ultrasonic frequency. Using immunohistochemical staining, we analyzed the expression pattern of auditory related genes in the chordotonal organ. Quantitative real time-PCR was used to compare the expression levels of auditory related gene depending on the time of exposure to ultrasonic sounds.

감귤그린병(Citrus Huanglongbing Disease)은 전 세계적으로 감귤산업에 가장 큰 피해를 주는 병해로 국내 미분포종인 귤나무이(Diaphorina citri Kuwayama)가 매개충으로 알려져 있다. 바이러스에 감염된 나무는 5~12년내에 대부분 고사하며 열매는 크지 못하고 색이 들지 않는 증상을 보여 상품성을 저해시킨다. 현재 접목, 묘목, 매개충등의 유입으로 전 세계적으로 확산되고 있는 실정이다. 귤나무이의 국내 분포가능성이 꾸준히 제시되면서 유입 대비를 위한 예찰방법 및 동정법 개발이 필요한 실정이다. 제주도에는 총 33종이 분포하는 것으로 알려져 있으며(권, 1983; Cho & burckhardt, 2017) 2016년부터 지금까지의 조사로 11종의 나무이와 10종의 기주식물을 확인되었다. 귤나무가 속해있는 운향과를 기주로 하는 나무이는 발견되지 않았다.