CBD and one of key character The Convention on Biological Diversity (hereinafter CBD), adopted in 1992, play unique role for global environmental policy in the world. Comparing to other nature conservation convention like CITES and Ramsar which have listing mechanism on specific species or sites, CBD provide opportunity for setting challenge, priority on biodiversity in global, regional and local level and discussing solution. One of uniqueness of CBD is broad range of agenda. Conference of Parties (COP) to the CBD deals nature conservation policy, land use planning (agriculture), use of nature (eco-tourism and customary/traditional knowledge on nature and its use) and scientific and technological matter on nature (Genetically modified species and genetic resource use) and made decision by consensus of 193 countries and region. Japanese Civil Society Strategy to engage CBD Process Towards CBD-COP10 in Japan in 2010, Japan Civil Network for CBD (JCN-CBD) was established to response to such wide scope of CBD and had 17 working group. The network was supported by scientist. Natural science supported better understanding on biodiversity, appropriate advocacy work and making communication based on science. JCN-CBD focused their role as information center (gather, consolidate, arrange and make easy-communication tool from them) and arranging the synergies with working group so that can promote engagement of civil society to CBD process. After COP10, to support strategic plan and 20 Aichi targets which was most important outcome and common global target about biodiversity in UN system, Japan Committee for IUCN are promoting Nijyu-maru Project which is a kind of campaign collecting commitment for contributing Aichi biodiversity targets and got 242 in commitment actions and from 178 organization. Key message People in host country of COP can easily access and influence to the global discussion on biodiversity through its preparation process. I would like to emphasize the benefit to engage it and importance of the role of natural sciences.

This study purposed to provide a scientific base for understanding the effect of therapeutic intervention using motivation on chronic stroke patients’balance and determining whether it is applicable as a new therapeutic intervention. For this study, we sampled 38 chronic stroke patients, and divided them randomly into a motivation training group(n=20) and a control group(n=18). To the control group was applied neurological physiotherapy 5 times a week, and 30 minutes each time, and to the motivation training group was applied neurological physiotherapy and then, additionally, a Nintendo Wii-Fit program 3 times a week, and 30 minutes each time. Before and after the experiment, the subjects’dynamic balance was measured with functional reach test(FRT), timed up & go test(TUG), and 10m gait test, and their static balance was measured with the Romberger Test. When dynamic balance ability was compared between before and after the experiment and between the motivation training group and the control group, significant difference was observed in the results of FRT, TUG, and 10m walking test between before and after the intervention(p<.05). As to static balance, in addition, body balance movement distance was not significantly different. Therapeutic intervention using dynamic motivation was found to be more effective than the control group in improving dynamic balance.

This study is a cross-sectional study for conscripts with chronic low back pain, where it is designed to investigate general characteristics that conscripts with chronic low back pain carry, and it is also aimed to analyze the correlation as well as discrepancy within pain, depression and body mass index depending on their ranks. In this study, 90 Korean conscripts with chronic low back pain participated. And in order to analyze general characteristics as well as pain, depression and body mass index of the subjects, this study adopted a survey questionnaire upon general characteristics, and it also employed Visual Analogue Scacle(VAS) and Center for Epidemiological Studies Depression(CES-D) Scale which have proven their credibility, where BMI values for the subjects were calculated based on 'Asian Pacific standard' Body Mass Index(BMI). From the general characteristics exhibited by the subjects through this study, average age and average height of the subjects were 20.78±1.41 and 175.97±5.34 respectively; and corporals displayed the highest ratio of 42% with the chronic low back pain while 40% of the total subjects experienced the duration of the illness less than 6 to 9 months. Moreover, 57.8% of all subjects were reportedly suffering moderate pain, and 48% exhibited depression while 49% carried BMI value between 18.5 and 22.9kg/m2(normal range). Based on the analysis of correlation among pain, depression, discrepancy of BMI depending on the ranks and each independent variable, it appears that there is no significant difference amongst the ranks, and there is no correlation between independent variables; however, it was obvious that all groups divided by the ranks had a moderate pain(45-74mm), depression(≥16) and BMI value in normal range(18.5-22.9).

The purpose of this study is to provide the case of influence of myofacial release(MFR) and cryotherapy in acute whiplash injury. The subjects were consisted of 3 adults(2 males, 1 female) who had been diagnosed with whiplash injury. The performance period was 1 weeks, 6 times and 1 time is 40min(MFR: 20min, cryotherapy: 20min). We measured neck pain(Visual analogue scale, VAS) and cervical alignment(cervical curvature & line of gravity). All measurements of each subject were measured at pre-treatment and post-treatment. The results are listed below. The neck pain(VAS) index decreased from 7.54cm to 4.11cm. The cervical curvature increased from 23.62°to 29.76°. The line of gravity decreased from 8.03mm to 3.66mm. Based on the results, it can be suggested that both MFR and cryotherapy can be used to neck pain and alignment in acute whiplash injury.

With the increased use of online learning in language education, the importance of videoconferencing lessons has recently received much attention in improving English speaking skills in Korea. This study examines the aspects of videoconferencing lesson that were helpful for improving students’ pronunciation. Six middle school students in Korea were assigned to two videoconferencing sessions with pre- and post-tests, and they had opportunities for interviews and selfreports after the two sessions. During the pre- and post-test, students pronounced words from a screen, distinguishing specifically between /r/ versus /l/ and /b/ versus /v/. In the interviews and self-reports, they described the factors in the videoconferencing lesson that were most helpful for their English learning. This study examined the appropriateness of videoconferencing tools and found that students’ self-assessment, more teacher feedback, and the flexibility of the technology were most helpful for improving Korean students’ English pronunciation.

The present study investigates the use of signaling nouns (SNs) in published and Korean graduate student academic writing in applied linguistics. A set of 35 nouns was examined for their frequency as SNs, while the six most frequent SNs in published writing were subjected to detailed analyses of realization patterns. The results indicated that the nouns function as SNs in fifteen percent of the time of their total use and that students overall used a greater number of SNs than did published authors. Despite considerable overlap in the most frequent SNs, there was evidence that students rely on a narrower range of SNs than published writers. Published authors were differentiated from students in employing more anaphoric SNs, which contributed to cohesiveness and organization of text through the effective encapsulation of the preceding stretch of discourse. This study challenges the previous claim that the frequency of SNs positively correlates with the writing proficiency. Some pedagogical implications are drawn for academic writing instruction.

This study was performed to evaluate antimicrobial resistance of food-borne pathogens isolated from retail meat in Korea. A total of 157 samples of beef, pork, and chicken were collected and analyzed for E. coli, Salmonella, Campylobacter. Resistances to tetracycline were declined in accord with reduced usage of tetracycline in live stock production. E. coli stains from chicken meat had higher multi-drug resistance ratio than strains from other meat. One extended spectrum beta lactamase (ESBL) producing E. coli and two ESBL producing Salmonella were identified in this study. ESBL producing Salmonella strains were confirmed to carry CTX-M-1 type genes.

Streptococcus mutans (S. mutans) is a facultative anaerobic bacterium mainly found in the oral cavity and is known to contribute to tooth decay and gingivitis. Recent studies on intestinal microbiota have revealed that microorganisms forming a biofilm play important roles in maintaining tissue homeostasis through their own metabolism. However, the physiological roles of oral microorganisms such as S. mutans are still unclear. In our current study, we identified that constituents released from S. mutans (CR) reduce arecoline- mediated cytotoxicity without producing toxic effects themselves. Arecoline, as a major alkaloid of areca nut, is known to mediate cytotoxicity on oral epithelial cells and induces a sustained intracellular Ca2+ ([Ca2+]i) increase that is cytotoxic. The exposure of human gingival fibroblast (HGF) cells to CR not only inhibited the sustained [Ca2+]i increase but also the initial [Ca2+]i elevation. In contrast, CR had no effects on the gene regulation mediated by arecoline. These results demonstrate that S. mutans has physiological role in reducing cytotoxicity in HGF cells and may be considered a novel pharmaceutical candidate.

In porcine embryo culture, one of reactive oxygen species (ROS) is harmful factors that are made during in vitro culture. To decrease the detrimental effect of ROS on embryo development, superoxide dismutase, catalase and glutathione peroxidase could be used in the embryo culture. Out of these antioxidants, 7,8-dihydroxyflavone (7,8-DHF) was reported its antioxidant effects to prevent the glutamine-triggered apoptosis. Therefore, this study was performed to investigate the most appropriate concentration of 7,8-DHF in porcine embryonic development. For that, 5 different concentration (0, 0.1, 0.5, 1, 2 uM) of 7,8-DHF was supplemented in the porcine IVM media and then maturation and blastocyst formation rates were compared among 5 groups. In maturation rates of porcine oocytes, significant higher maturation rates was shown in the 1.0 uM group compared with another 4 groups (83.3 ± 2.1 vs. 80.7 ± 1.4, 79.8 ± 1.4, 78.3 ± 1.2, 79.4 ± 1.6), respectively (P<0.05). In the embryo culture, 1.0 uM group also showed the significant higher cleavage rates (76.8 ± 3.1 vs. 62.1 ± 5.0, 65.7 ± 4.0, 68.6 ± 3.7, 64.6 ± 4.0%) and blastocyst formation rates - (39.6 ± 4.0% vs. 28.6 ± 3.3, 31.1 ± 3.9, 29.3 ± 2.5, 39.6 ± 4.0, 26.4 ± 3.2%), respectively (P<0.05). There was no significant difference among 5 groups in the cell number of blastocyst (P<0.05). In conclusion, supplement of 1.0 uM of 7,8-DHF was effective to increase the porcine embryonic development competence as antioxidant to ROS.

Transcription activator like effector nucleases (TALENs) are artificial restriction enzymes generated by fusing a TALE DNA binding domain to a DNA cleavage domain which remove and introduce specific genes to produce transgenic animals. To investigate the efficient laboratory techniques for the injection of TALEN mRNA, pEGFP-N1 commercial plasmid were microinjected into porcine parthenogenetic and in vitro fertilization (IVF). In Experiment 1, to investigate injection time, compared 4 different time durations (2 hr, 4 hrs, 6 hrs & 8 hrs) after post activation of parthenogenetic embryos and after 6 hrs of co-incubation with sperms in IVF embryos. There were significant difference (P<0.05) in development to the blastocysts (4.4, 8.9, 3.9, 0.6%), GFP expression in blastocysts (1.3, 5.7, 2.3, 0.0%) which injected after post activation of 4 hrs compared with other 3 groups. IVF embryos after 2 hrs and 4 hrs injected were expressed GFP significantly higher than rest of two groups (P<0.05). In Experiment 2, compared development of 2 different concentrations (20 ng/μl and 50 ng/μl) of EGFP injection. There were significant difference (P<0.05) between two treatments which has higher cleavage (58.8 vs 41.9%), blastocysts development rate (13.0 vs 11.1%) and GFP expressed blastocysts (5.7 vs 0.0%) in 20 ng/μl than the 50 ng/μl in parthenogenetic embryos. In IVF embryos, only 20 ng/μl injected embryos were expressed GFP (4.2%) after 7 days of incubation and 77.3 vs 64.7% of cleavage, 26.4 vs 23.5% development to blastocysts. In Experiment 3, three different volumes (5, 10 and 20 pl) were microinjected into porcine embryos to determine the most appropriate volume. Out of 3 groups, significantly higher development rates of cleavage (68.3, 58.0, 29.3%), blastocysts (11.7, 12.7, 0.5%) and GFP expressed blastocysts (2.9, 7.8, 0.0%) were shown in the 10 pl group (P<0.05). In conclusion, these results imply that 20 ng/μl concentration, 10 pl of volume and injection at 4 hrs after post activation for parthenogenetic and 2∼4 hrs after IVF, 20 ng/μl concentration and 10 pl volume for IVF embryos were more effective microinjection conditions.

Considerable research in dieting has examined the goal priming effect that promotes successful dieting. However, priming literature found that the process of which priming is led to behaviors involves not only the priming construct itself but the momentary self-concept activated by the priming. Based on this notion, studies examined whether the active self-concept as an “unsuccessful dieter” or “successful dieter” determines the priming-to-behavior effect in dieting. Study 1 used contexts of the prime as manipulation for participants’ active-self following the prime and measured their chronic self-concept in dieting (rated on the Perceived Self-Regulation Success [PSRS] in dieting scale) as a moderator. The result supported the active-self account for the effect of the dieting prime. The interaction of the PSRS scores and the priming contexts was found in the dieting behavior of participants. Participants whose chronic self-concept in dieting is unsuccessful (the low PSRS) showed the goal-congruent priming effect only in the condition where the priming context reminded them of their self-concept in favor of dieting success. In the context where their dieting failure in the past became salient, however, the participants with the low PSRS were more likely to succumb to tempting food. In contrast, eating behaviors of the high PSRS participants were independent of the contexts. Study 2 tested a possible mechanism from the active-self to the behaviors. It is concluded that the active-self operates in the priming process by influencing anticipatory thoughts rather than automatic responses toward foods. The implications for understanding repeated dieting failures and designing practical interventions to help dieters are discussed.

한국산 무당벌레붙이과(Endomychidae Leach)를 분류학적으로 검토하였다. 한국산 무당벌레붙이과에는 3아과, 7속, 10종이 포함되어 있는데, 그 중 Endomychus gorhami (Lewis) Eumorphus quadriguttatus pulchripes Gerstaecker, Lycoperdina castaneipennis Gorham, Mycetina ancoriger Gorham 등 4종을 국내에서 처음으로 보고한다. 또한 Lycoperdina castaneipennis의 숙주버섯을 처음으로 보고한다.

Asymmetric sitting posture may cause asymmetric buttock pressure and unilateral low back pain (LBP). The purpose of this study was to compare the differences of buttock pressure between both sides, and pelvic angle (sagittal and coronal planes) during typing in a sitting position on a pressure mat (Baltube) in individuals with and without unilateral LBP. Ten subjects with unilateral LBP and ten subjects without unilateral LBP were recruited for this study. Buttock pressure was measured using a pressure mat and pelvic angles were measured using a palpation meter. The subjects performed typing in a sitting posture for 30 minutes. Pressure data were collected and averaged at initial term (from start to first minutes) and final term (last minutes of 30 minutes). Angles of pelvic tilting were measured after 30 minutes typing. Pressure asymmetry values (difference in pressure between both sides) were calculated at the initial and final terms. A two-way analysis of variance was used to compare the differences between the initial and final pressure asymmetry values in subjects with and without unilateral LBP. An independent t-test was applied to compare the pelvic tilt angles between the two groups. To compare the change of pressure from the initial term to the final term between the symptomatic and asymptomatic sides in the unilateral LBP group, a paired t-test was applied. In the unilateral LBP group, the pressure asymmetric value at the final term was significantly greater than that of the initial term (p<.05). The angle of pelvic tilting in coronal plane was significantly greater in the unilateral back pain group compared to the without unilateral LBP group (p<.05), however, there was no significant difference in the angle of pelvic tilting in the sagittal plane between the two groups (p>.05). In the unilateral LBP group, the change of pressure from the initial term to the final term was significantly less in the symptomatic side (-6.90 ㎜Hg) than the asymptomatic side (5.10 ㎜Hg). This asymmetric sitting posture may contribute to unilateral LBP in the sitting position. Further studies are needed to determine if asymmetric weight bearing in sitting causes unilateral LBP or if unilateral back pain causes asymmetric weight bearing, and if the correction of asymmetric weight bearing in sitting can reduce unilateral LBP.

This study investigated the densification behavior of rhenium alloys including W-25 wt.%Re and Re-2W-1Ta (pure Re) during sintering. The dilatometry experiments were carried out to obtain the in-situ shrinkage in H2 atmo-sphere. The measured data was analyzed through shrinkage, strain rate and relative density, and then symmetricallytreated to construct the linearized form of master sintering curve (MSC) and MSC as a well-known and straightforwardapproach to describe the densification behavior during sintering. The densification behaviors for each material were ana-lyzed in many respects including apparent activation energy, densification parameter, and densification ratio. MSC witha minimal set of preliminary experiments can make the densification behavior to be characterized and predicted as wellas provide guideline to sinter cycle design. Considering the results of linearized form and MSC, it was confirmed thatthe W-25 wt.%Re compared to Pure Re is more easily densified at the relatively low temperature.

In the present work, WO3 and WO3-TiO2 were prepared by the chemical deposition method. Structural variations, surface state and elemental compositions were investigated for preparation of WO3-TiO2 sonocatalyst. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and transmission electron microscopy (TEM) were employed for characterization of these new photocatalysts. A rhodamine B (Rh.B) solution under ultrasonic irradiation was used to determine the catalytic activity. Excellent catalytic degradation of an Rh.B solution was observed using the WO3-TiO2 composites under ultrasonic irradiation. Sonocatalytic degradation is a novel technology of treating wastewater. During the ultrasonic treatment of aqueous solutions sonoluminescence, cavitaties and "hot spot" occurred, leading to the dissociation of water molecules. In case of a WO3 coupled system, a semiconductor coupled with two components has a beneficial role in improving charge separation and enhancing TiO2 response to ultrasonic radiations. In case of the addition of WO3 as new matter, the excited electrons from the WO3 particles are quickly transferred to TiO2 particle, as the conduction band of WO3 is 0.74 eV which is -0.5 eV more than that of TiO2. This transfer of charge should enhance the oxidation of the adsorbed organic substrate. The result shows that the photocatalytic performance of TiO2 nanoparticles was improved by loading WO3.

This report describes a rare case of primary rectal mature teratoma in a 56-year-old woman. She was referred to the outpatient clinic with a large pedunculated rectal mass, which was found during a regular health check-up. Polypectomy was performed and microscopic findings showed various structures derived from all three germ cell layers. Epidermis, hair follicles, sebaceous glands, eccrine glands, and apocrine sweat glands, with some scattered melanophages and lymphocytes were present as ectodermal derivatives. Smooth muscle fibers, blood vessels, and fibrous and adipose tissues were found as mesodermal derivatives. In addition, thyroid follicles, mucinous glands, and bronchial respiratory epithelium with peribronchial glands were detected as endodermal derivatives. She is healthy and has shown no evidence of recurrence or distant metastasis for 25 months post-surgical resection. Primary rectal teratomas are generally benign and primarily affect women. Therefore, minimally invasive surgical procedures, such as endoscopic polypectomy for a pedunculated polyp and segmentectomy for a larger mass, are satisfactory in most cases. Induction of primary rectal teratomas has been suggested to occur mainly by errors in a single germ cell after the end of meiosis I; in addition, it has also been suggested that the difference in gender incidence may be associated with differences in sex chromosomes between males and females rather than with anatomical proximity between ovary and rectum.

The anti-proliferative efficacy of t,t-conjugated linoleic acid (t,t-CLA), c9,t11-CLA, and t10,c12-CLA was compared in several human cancer cell lines. Gastric NCI-N87, liver Hep3B, pancreas Capan-2, and lung NCI-H522 cancer cells were incubated with 50 μM CLA isomers over a period of 6 days. The t,t-CLA inhibited the growth of all cancer cell lines to different extents, but c9,t11-CLA and t10,c12-CLA inhibited or stimulated the growth of the cancer cell lines. NCI-N87 cells were the most sensitive to growth inhibition and apoptosis from all CLA isomers tested. In NCI-N87 cells, CLA isomers reduced the release of arachidonic acid (AA) via the inhibition of cytosolic phospholipase A2 (cPLA2 ) activity, consequently reducing the production of PGE2 through the inhibition of cyclooxygenase-2 (COX-2). The efficacies of CLA isomers were in the following order (from most to least effective): t,t-CLA, t10,c12-CLA and c9,t11-CLA. Overall, these results imply that the anti-proliferative efficacy of t,t-CLA on cancer cells, especially NCI-N87 cells, was greater than other CLA isomers due to its induction of apoptosis through the inhibition of cPLA2 and COX-2 activities.

Antimicrobial peptides (AMPs) are an important component of innate defense mechanisms with broad-spectrum activities against various pathogenic microorganisms, including Gram-positive and Gram-negative bacteria, fungi, and viruses. Antibiotic resistance has become a pervasive and global health burden, resulting in the immediate need to develop a new class of antibiotic substances. We screened a 16-mer random peptide library using the yeast two-hybrid system with Beclin 1 as bait and found that two 16-mer peptides (named P4 and P30) appeared to interact with Beclin1 in the β-gal assay. The two candidate cDNAs were introduced into the yeast secretory system of Pichia pastoris and their expression induced in the presence of methanol. Spectrophotometric analysis and Disc clear zone assay using the supernatant of the yeast growth media showed that both of the two peptides had strong activities against Staphylococcus aureus, MRSA (methicillin resistance Staphylococcus aureus), MRSA2242, and MRSA-2250, but no effect on commensal Lactobacillus strains. PCR analysis of the genomic DNA of transformed Pichia pastoris using AOX1 primers revealed that the two cDNAs were integrated into the genome at the AOX1 locus. Our result suggests that these peptides could be developed as a useful alternative to classic chemical antibiotics.

Our previous research on sulfated polysaccharide purified from Ecklonia cava, a brown alga found in Jeju island, Korea, showed that sulfated polysaccharides modulate the apoptotic threshold of intestinal cells, thereby preventing intestinal damage caused by ionizing radiation. In this study, we investigated the ability of sulfated polysaccharide to augment restoration of small intestinal stem cells from γ-ray-induced damage. In our results, sulfated polysaccharide treatment increased the numbers of Ki-67-positive cells as well as inducible nitric oxide synthase (iNOS)-expressing cells in the small intestine compared with those of irradiated only mice. Meanwhile, exposure to irradiation increased the number of paneth cells, which are frequently associated with intestinal inflammation, whereas sulfated polysaccharide treatment reduced the number of paneth cells in the small intestinal crypt. Conclusively, our data suggest that reduction of iNOS-expressing cells and paneth cells in sulfated polysaccharide-treated mice contributes to the inhibition of radiation-induced intestinal inflammation.

Wide-field JHKs images obtained with the SIRIUS near-infrared camera of the IRSF 1.4m telescope are used to examine the tidal structures of the spatial stellar configuration around six metal-poor ([Fe/H]< −1.0) globular clusters located within 3 kpc from the Galactic center. The radial surface density profiles are obtained from the surface photometry of the cluster images and the star counting for the photometric data. For the star counting, candidates of cluster member stars are selected with an filtering algorithm in color-magnitude diagrams. We find that the six target clusters show tidal overdensity features in the radial surface density profiles. There is a break inside the tidal radius for each cluster, and the profile in the outer overdensity region is characterized by a power law. Two- dimensional density maps of all the clusters show distorted asymmetric stellar configurations in the outer region. In five out of the six target clusters, the overdensity features are likely to be associated with the effects of the Galaxy dynamical interaction and the cluster space motions. The observed tidal configurations of stars suggest that several metal-poor clusters in the Galactic bulge are possibly surviving remnants of mergers to build the old stellar system of the Galactic bulge.