Contaminated soils and water by heavy metals have posed a serious threat to environment and human health, which can be solved by both physicochemical and biological methods. Phytoremediation has recently become a topic of scientific research and gained much attention as a cost-effective and eco-friendly alternative. Many kinds of hyperaccumulator plants have been applied to deal with heavy metal contaminants such as As, Cd, Pb, Zn and various radionuclides. In recent study, a seedling vigor index (SVI) model, which uses specific hyperaccumulator plants (i.e., sunflower) as test subjects, is proposed to predict the phytotoxicity of heavy metals. However, whether this mathematical model can be commonly used has not been cleared yet. In this study, rapeseeds were treated with Cd, Pb, Ni, Zn to validate the SVI model which was proposed by some scholars previously. The phytotoxicity of heavy metal was evaluated using seed germination test and seedling length measurement. The test shows that germination percentage and seedling length decreased when heavy metal concentration increased. According to the results of regression analysis, heavy metal concentration is linear to germination percentage, root and shoot length, corresponding with SVI model. It can be concluded that the SVI estimation model obtained from sunflowers can also be applied to rapeseeds to describe the relative effects of heavy metal concentration on plants growth and development.. However, further experiments using more hyperaccumulator plants to validate the applicability of the SVI model are necessary.

The purpose of this study was to investigate the effect of the preparation method on CeO2-promoted Cu/γ -Al2O3 catalysts for the high temperature shift reaction using simulated waste-derived syngas (H2 + CO). To investigate the effect of preparation method on the CeO2-promoted Cu/γ-Al2O3 catalyst, we compared catalytic performance over Ce/Cu/γ-Al2O3, Ce-Cu/γ-Al2O3, Cu/Ce/γ-Al2O3, and Cu/γ-Al2O3 catalysts, and tried to explain the differences in catalytic performance with various characterization methods. The physico-chemical properties of the CeO2-promoted catalysts were characterized using surface spectroscopies such as BET, XRD, TPR, XPS, Raman spectroscopy, photoluminescence spectroscopy, and N2O-chemisorption. The catalyst characterizations were correlated with activity results in the high temperature shift reaction.

This research assessed the disparity in anion generation according to the vertical structure of a Zelkova Serrata tree for the purpose of creating a pleasant and green city environment. Measurements for the study were conducted between July and August of 2014 in Chung-ju in the central region of the Republic of Korea. The average anion generation of vertical structure trees during active photosynthesis periods was: L Section (839.0 ea/cm3) > M Section (664.6 ea/cm3) > U Section (472.0 ea/cm3). According to DMRT analysis, significant difference was found in the average between the L, or M Locations and the U Locations. During dormant photosynthesis periods, records showed that the anion production at the M Location (1,212.5 ea/cm3) > L Location (1,050.4 ea/cm3) > H Location (844.1 ea/cm3), According to DMRT analysis, the difference within each location was significant for α=0.05. In a comprehensive analysis of the weather factors in each vertical structure, anion generation during active photosynthesis periods showed a positive correlation with solar radiation and a negative correlation with wind speed. Dormant photosynthesis periods showed negative correlations with both solar radiation and temperature, and positive correlations with relative humidity and wind speed. Predictions from a multicenter retrospective study showed that during active photosynthesis periods, Y1 = 282.443X1 + 512.07, and Y2 = 314.337X1 + 16.913X2, while during dormant photosynthesis periods, Y1=391.009X1 + 840.043, and Y2=351.412X1 + 32.765X2.

The objective of this study was to determine the CH4 oxidation factor (%) and the CH4 oxidation rate (g m−2 d−1) in landfill cover soil. To quantify in-situ rates of CH4 oxidation, CH4 and CO2 fluxes were measured on a landfill site using the static chamber technique. The CH4 oxidation factor obtained in this study through the mass balance method ranged between 41% and 61%, which is much higher than the Intergovernmental Panel on Climate Change (IPCC) default value of 10%. The higher CH4 oxidation factor derived in this study can be explained by the CH4 bottom flux in addition to the soil texture. The CH4 oxidation factors were observed to increase with decreases in CH4 bottom flux. Therefore, when CH4 bottom fluxes are high in a landfill, using a gas collection system can enhance CH4 oxidation factor. The CH4 oxidation rates were estimated to range from 16.6 g m−2 d−1 to 20.8 g m−2 d−1. In addition, this study was conducted to evaluate the effects of vegetation on the CH4 oxidation factor. The results showed that the CH4 oxidation factors for bare soil, vegetated soil, and soil adjacent to a gas well were 57%, 70%, and 44%, respectively. The results indicate that vegetation on landfill covers can increase the CH4 oxidation factor because of increasing soil porosity.

This study presents a system dynamics methodology to evaluate quantitatively the effect of the Korean government’s development policy, such as tax reductions, on the industrial economy. System dynamics is often perceived as an optimized means to identify the dynamic inter-relationships among various factors of development policies, and in particular the industrial characteristics and uncertainties of the coastal shipping industry. The results of simulations used in this study shows that the impact of development policies such as tax reductions would increase shipping demand for about 4 years, and that tax incentives could raise the demand volume for cabotage cargo from 5.26 to 11.11%, through the available freight- down by 90∼95% points. The system dynamics approach used in this paper represents an initial attempt to use this methodology in studies of the coastal shipping industry. On the basis of our simulations, the industrial effects of other development policies, such as ship financing support, investment of social overhead, or crew supply, could also be analyzed effectively. Additionally, it should be possible to extend these results by developing a comprehensive model encompassing these various analyses.

Accidents and incidents caused by poor performance in the maritime industry suggest the need to find ways to improve performance in order to operate effectively and improve safety. The data for this study was collected from maritime students and involves the performance and levels of intrinsic and extrinsic motivation. Various motivational factors were identified and the ways in which these factors relate to performance was analyzed. The importance of considering motivation when recruiting new trainees and workers, and continually looking for ways to increase intrinsic motivation, is stressed throughout this paper. The results suggest that high levels of good quality motivation correlate positively with better performance.

Purpose - The study sought to examine the relationship between individual characteristics and system features and Human Resource Information System (HRIS) success in Japanese manufacturing companies in Malaysia. Research Design, Data, and Methodology – This study adopt quantitative approach to investigate the relationship between individual characteristics and system features and HRIS. Toward this objective, a total of 700 questionnaires were mailed to a representative of the organization. A total of 187 questionnaires were returned, and only 145 were usable for further analysis, representing a response rate of 20.71%. Result - Results indicated that individual characteristics and two dimensions of system characteristics (ease of use and training) were significantly related to HRIS success. Unexpectedly, the results showed that the third dimension of system features (documentation) was unrelated to HRIS success. Conclusions - The results partially support the underlying arguments that individual characteristics and system characteristics have significant influences on HRIS success. The finding suggests that HRIS success in the organization can be generated as a result of good implementation of system support and employees’ readiness to apply HRIS in their jobs.

Background : Ginseng is a perennial, and damage occurs due to various diseases and pests. As a result, yield decreases and quality deteriorates. In particular, it is impossible to be repeatedly cultivated due to root rot, soil-borne disease. For this, in order to control root rot and repeatedly cultivate ginseng by using soil fumigation from virgin soil, this experiment was conducted. Methods and Results : This experiment was performed in ginseng farm field, Geumsan-gun, Chungnam province. And total area was 3ha. And the experiment was conducted in soil where apple trees had been grown. Apple trees were rooted up in 2006. And soil was fumigated in May 2007. With regard to soil fumigant, soil was treated with Basamid of 40kg/10a. After soil fumigation, 5 ton/10a of cattle manure fermented for 1 year was put in the soil in June, and then the soil was cared for by being plowed and rotary-tilled as occasion. Ginseng seeds were sowed in the soil cared for in October 2007. And 4-year-old ginseng was harvested in 2011. The soil from which ginseng was harvested was fumigated by the same method in May 2012, and then ginseng seeds were sowed in 1.5ha in October 2012 and in 1.5ha in October 2013. In October 2013, 3-year-old and 4-year-old ginseng was harvested. And ginseng growth characteristics and root rot incidence was examined. It was shown that 4-year-old ginseng yield after the fumigation of virgin soil was nearly 2 times as high as that of conventional virgin soil cultivation. And root rot didn't occur. After that time, the soil was fumigated again. And when ginseng was cultivated, 3-year-old and 4-year-old ginseng yield was 650kg and 960kg per 10a. And it was shown that root rot incidence was 1.3% and 15.3% in 3-year-old and 4-year-old ginseng respectively. Conclusion : This study showed the results where ginseng can be cultivated repeatedly if soil was fumigated again after ginseng was harvested following the soil fumigation from virgin soil so as to control the ginseng root rot and cultivate ginseng repeatedly.

Background : Korean Indigenous Hylotelephium erythrostictum is widely distributed in South Korea and is used in Korean traditional medicine. In this study, the phylogenetic analysis of Korean native Hylotelephium erythrostictum and related plants on Internal Transcribed Spacer (ITS) sequences were investigated to distinguish its origin. Methods and Results : The phylogenetic analysis of 6 species of Hylotelephium were investigated by ITS. The dendrogram was constructed by UPGMA(Unweighted Pair Group Method with Arithmetic Mean) clustering algorithm based on genetic similarity of ITS. In the ITS sequence analysis, the size of total was varied from 676 to 779 bp. The size of ITS 1 was rated at 287bp, while ITS 2 was rated at 123bp. The G+C content of ITS region was ranged from 60 to 66%. In the ITS tree, six species of Hylotelephium were monophyletic, and H. viviparum was the first branching within the clade. Conclusion : H. spectabile formed a clade with H. erythrostictum, while H. verticillatum formed with H. viviparum.

Background : Gentinae Macrophyllae Radix is one of the traditional medicines originated from the roots of multiple plants, Gentiana macrophylla Pall., Gentiana straminea Maxim., Gentiana crassicaulis Duthie ex Burkill and Gentiana dahurica Fisch., in Gentianaceae. Multi-origin traditional medicine usually has adulteration problem based on the morphological similarity and/or misunderstanding the species. Therefore, accurate and reliable identification criteria to ensure drug safety and quality is necessary. Methods and Results : We collected four original species of Gentinae Macrophyllae Radix from plantations and markets in China and Korea. DNA barcoding with four barcoding markers (Internal Transcribed Spacer (ITS), rbcL, trnL intron, trnL-F intergenic sapcer) was performed. Intra-specific variation was observed in ITS nucleotide sequence however, successfully distinct four original species based on the nucleotide discrepancy while trnL intron has no difference. trnL-F intergenic spacer has two transitions(T→C and A→G) sites only in G. crassicaulis and rbcL shows one transition(C→T) site in G. dahurica and G. macrophylla. Phylogenetic relationship analysis of the Gentinae Macrophyllae Radix revealed two major clades – clade I including three groups, G. macrophylla, G. straminea and G. dahurica, and clade II including G. crassicaulis. This aspects was shown more clear with multi-region combined analysis. Conclusion : DNA barcoding will be accurate and powerful criteria for the analysis the origin of Gentinae Macrophyllae Radix. However, single region analysis might be deficient such as trnL intron, rbcL and trnL-F intergenic spacer results in this research. Multi-region combined analysis based on the multiregional DNA barcode markers will be overcome the disadvantage and also increase the precision.

Background : Nasturtium officinale, belongs to the Brassicaceae, is a perennial plant growing in and around natural watersystem. It is commonly called watercress and commercially consumed as a salad crop in many countries. Hairy root cultures (HRCs), transformed by Agrobacterium rhizogenes (A. rhizogenes), have been noted for an experimental model system in plant metabolic engineering for the synthesis of natural products since hairy roots have fascinating properties including biochemical and genetic stability, high biosynthetic capacity for secondary metabolites and rapid growth rates. Methods and Results : The dry weights (DW) of watercress hairy roots was measured after 4-days freeze dryer. The highest weight was recorded after HRCs in SH and half-strength SH medium, followed by the levels of DW in MS and half-strength medium. However, the level of DW after HRCs in half-strength medium was lowest. SH medium is the most suitable for the growth of watercress hairy roots. Glucosinolate contents in the hairy roots varied responding to the basal media. 1/2 SH basal media resulted in the lowest value of total glucosinolate. MS basal media, however, resulted in the highest value of total glucosinolate, as well as the highest accumulation of each glucosinolate. The hairy roots cultured in 1/2 MS basal media showed the second highest value of total glucosinolate, followed by B5, SH and 1/2 B5. The accumulation of 4-Methoxyglucobrassicin and gluconasturtiin was also secondly higher in the hairy roots in 1/2 MS. In contrast, the level of 4-Hydroxyglucobrassicin and glucobrassicin were the second highest after culturing in SH and B5, respectively. Conclusion : In this study, media played a main role in growth and glucosinolate accumulation in watercress hairy roots. SH and half-strength SH media enabled the rate growth of hairy roots to be highest. In contrast, the highest accumulation of glucosinolate was recorded after HRCs in MS media. The current study suggests HRCs of watercress could be one of an effective alternative approaches for the enhanced production of glucosinolates

Background : Ancanthopanax senticosus Fruits Extracts were studied in order to develop as functional pigment. The extracts were evaluated for physicochemical properties and biological activity. Methods and Results : Ancanthopanax senticosus fruits extracts were extracted by the rate of ethanol and distilled water in 1% citric acid. The extracts were evaluated for physicochemical properties by chromaticity, total sugar content, free sugar content, acidity, total anthocyanin contents. Total sugar and acidity were 0.10 brix and 0.44% respectively. Fructose and glucose were 1.54% and 0.36% respectively. The 60% ethanol extracts showed the highest extraction yield and total anthocyanin content. The α-glucosidase inhibitory activity of 60% and 80% ethanol extracts higher than other extracts. Conclusion : These results suggest that Ancanthopanax senticosus fruits extracts may be useful purple pigment for anti-diabetes activity.

Background : Aerial parts of Angelica gigas were studied in order to develop as functional pigment. The extracts were evaluated for physicochemical properties and biological activity. Methods and Results : Aerial parts of Angelica gigas were extracted by the rate of ethanol and distilled water. The extracts were evaluated for physicochemical properties by chromaticity, total sugar content, free sugar content, acidity, chlorophyll and carotenoids contents. Total sugar and acidity were 0.90 brix and 2.84% respectively. Chlorophyll and carotenoids contents were 0.24 and 0.04% respectively. The extracts were evaluated for biological activity by anti-inflammatory and anti-diabetes activity. Cell viabilities were measured by MTT assay. Effect of extracts on nitric oxide (NO) production from RAW264.7 cells was accessed by Griess reagent assay. Extracts to RAW 264.7 cells reduced amounts of nitrite by 76.0%. Conclusion : These results suggest that aerial parts of Angelica gigas Nakai may be useful pigment for anti-inflammatory and anti-diabetes activity.

Background : Agrimonia pilosa (A. pilosa) Ledebour has been registered in The Korean Herbal Pharmacopeia (KHP). In the recent study, A. Coreana showed antioxidant and anti-inflammatory effect. However, Studies of components in Agrimonia coreana (A. Coreana) Nakai was not much. So, we compared A. pilosa and A. coreana by High performance liquid chromatography (HPLC). we perfomed Thin layer chlromatography (TLC) including the anatomical characteristics by using microscope. Methods and Results : The anatomical characteristics of A. pilosa were similar to them of A. coreana. But, fascicular fivers of A. Coreana was broader than it of A. pilosa. TLC were performed to identify the Rutin and Apigenin-7-glucuronide compound. In the extract of Agrimoniae Herba, they were identified on the spot of Rf 0.2, 0.4 in Ethyl acetate - Formic acid – Water (8 : 1 : 1). The Rutin and Apigenin-7-glucuronide were analysed by HPLC/UV with Thermo Column (5 μm, 4.6 × 250 mm, C18), the column temperature at 40 ℃ and a diode-array detector (DAD) seted at 255 nm and 338 nm. The mobile phase was composed of water and acetonitrile containing 0.1% formic acid with the flow rate 1 mL/min. All compounds showed good linearity (R2>0.999) within test ranges. Agrimoniae herba was extrated by four kinds of extraction methods: MeOH, 50% MeOH, EtOH and water. The highest extraction rate occurred, when it was treated with 50% Methanol for refluxing extraction (60min). Content of Rutin was found to be 0.07±0.00 mg/g in A. pilosa and 0.02±0.00 mg/g in A. coreana. Content of Apigenin-7-glucuronide was found to be 0.12±0.00 mg/g in A. pilosa and 0.11±0.00 mg/g in A. coreana. Conclusion : The anatomical characteristics of A. pilosa were similar to them of A. coreana. Contents of Rutin and Apigenin-7-glucuronide in A. pilosa was higher than them in A. coreana slightly. But there were observed the similar patterns of Agrimonia pilosa Ledebour and Agrimonia coreana Nakai on the finger print anelysis.

Background : Campanula takesimana is a herb(Jabanpungnyeoncho) used traditionally in the korea private and we tried the development on a medicinal material. It was known that it has chlorogenic acid, as a immunoadjuvant activity. In this study we investigated the contents of the chlorogenic acid in Companula takesimana at different harversting time by the high performance liquid chromatographic(HPLC)-PDA. Campanula takesimana was collected on May maddle (Flower buds farmed), June middle (flowers opened) and July middle (seeds were mature). Methods and Results : HPLC analysis was carried out using X-bridge C18 column (5um, 250*4.6mm) and a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 1.0 ml/min. The optimum wavelength for the detection of the Chlorogenic acid was 330 nm using Photo Diode Array Detector. The contents of the chlorogenic acid in the Campanula takesimana extrat were 1.00% (May middle), 0.84% (June middle), 0.05% (July middle), respectively. As the different parts of Campanula takesimana, chlorogenic acid contents in May middle extrat were 2.73% (aerial part), 0.05% (root) and in June middle extrat were 1.61% (aerial part), 0.03% (root), 0.70% (flower) and in July middle were 0.13% (aerial part), 0.03% (root), 0.00% (seed), respectively. Conclusion : From the above results, the highes content of chlorogenic acid was observed in aerial part and May middle extract of Campanula takesimana.

Background : Haskap berries commonly refer to fruits of Lonicera caerulea L., recognized by the Japanese aborigines as the “The elixir of life.”. Due to their recent arrival on the North American market, haskap berries have not yet been positioned among other berries and compared in terms of their phytochemical content. And haskap berries have higher ascorbic acid and anthocyanin content than other berries known for their health-promoting benefits, such as blueberries. However, no study has reported on the antioxidant and anti-cancer activity of Lonicera caerulea stem. The purpose of this study is to present the current research on the chemical content, antioxidant and anti-cancer activities of Lonicera caerulea stem. Methods and Results : The stem of Lonicera caerulea L. ware dried in the shade at room temperature and extracted with 100% methanol. The extract was suspended in deionized water and partitioned sequentially with n-hexane, chloroform, ethyl-acetate and butanol (water saturated BuOH) fractions. Antioxidant activities were measured by determination of antioxidants, DPPH (2,2-diphenyl-1-picrylhydrazyl). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. All cell lines were purchased from the Korean Cell Line Bank (Seoul, Korea). All results were performed with three replications were processed statistically. By DPPH assay, the Lonicera caerulea L. the highest activity was obtained from the ethyl-acetate fraction (IC50=15.46 ㎍/㎖). By MTT assay, the chloroform fraction showed a significant growth inhibiting effect on MCF-7 (Human breast cancer, IC50=225.91 ㎍/㎖), COLO 205 (Human colon cancer, IC50=179.55 ㎍/㎖), but on AGS (Human stomach cancer) and other fractions it did not show effect. Conclusion : We demonstrated that Lonicera caerulea L. stem extract and fractions has antioxidant and antiproliferation activity in vitro. Further studies should identify the active constituents in Lonicera caerulea L stem to evaluate the potential in vitro antioxidant and antiproliferation activities of the extract.

Background : As a part of ongoing research to elucidate and characterize anti-inflammatory nutraceuticals, six kinds of plant extracts (aerial part of Nepeta cataria, leaves of Lonicera maackii, leaves of Platycarya strobilacea, flower of Fagopyrum dibotrys, flowers and fruits of Solanum nigrum, stem of Physostegia virginiana) were tested for their ability to suppress inflammation. The anti-inflammatory has been studied in lipopolysaccharide (LPS)-stimulated RAW264.7 cells which cells synthesized nitric oxide (NO) from L-arginine by nitric oxide synthase (NOS). In this study, NO synthesis inhibitory activity of six kinds of plant extracts on LPS-stimulated RAW 264.7 mouse macrophages was evaluated. Methods and Results : Six kinds of plant extracts were parceled out from RDA (Rural Development Administration). RAW 264.7 cells (1.5×105 cells/well) were seeded onto 96-well plates with DMEM media containing 10% FBS and 1% antibiotics. The cells were pretreated with the extracts and LPS-stimulated cells for 24 h. Cellular NO production was stimulated by adding 1 μg/mL of LPS. After incubation, Griess reagent was used to determine NO production. Absorbance was measured at 520 nm by microplate reader. NO synthesis inhibitory activity potential of these extracts was evaluated by assessing NO production by LPS-stimulated RAW 264.7 cells in the presence. As a result, inhibition rate of NO production was about 40% of L. maackii, 33% of F. dibotrys, 23% of P. strobilacea and 17% of P. virginiana. Meanwhile, there was no significant results in aerial part of N. cataria and flowers and fruits of S. nigrum. Conclusion : From the above results, we be able to confirm that leaves of L. maackii and flower of F. dibotrys appeared dose-dependent NO synthesis inhibitory activity and leaves of P. strobilacea appeared NO synthesis inhibitory activity in low-concentration. As screening NO synthesis inhibition of six extracts, they may be a good candidate for delaying the progression of human inflammatory diseases and warrants further studies.

Background : Despite the presence of various bioactive compounds in ginseng, there is lack of study on the variations of bioactive compounds in ginseng according to the cultivation of soil and the applied fertilizer types (or amount). Therefore, this study aims to examine the variations of 37 fatty acids (FA) and 8 vitamin E (Vit-E) vitamers in 6-year-old ginseng root cultivated in different soil types with different fertilizers regimes. Methods and Results : The profiling of 37 FAs and 8 Vit-E vitamers in 6-year-old ginseng roots was measured by gas chromatography coupled with a flame ionization detector, and then these results were statistically analyzed with chemometrics. The FA and Vit-E content in ginseng roots varied significantly with respect to soil cultivation conditions due to organic fertilizer types and amounts used. Unsaturated FA in ginseng is approximately 2.7 fold higher than the saturated FA. Linoleic, palmitic, and oleic acids were the most abundant FAs found in the ginseng roots. Also, the major Vit-E vitamer found in ginseng root is α -tocopherol. In particular, the application of rice straw compost or food waste fertilizer was increased to create nutritionally-desirable FAs and bioactive Vit-E in ginseng root. In addition, phytonutrient profiling coupled with chemometrics can be used to discriminate the cultivation conditions of ginseng. Conclusion : This preliminary study extends our understanding about the variations of FA and Vit-E in ginseng root depending on cultivation conditions. Hence, these results can be useful as basic information for reliable ginseng production containing high amounts of phytonutrients in a paddy-converted field.

Background : Recently, ginseng (Panax ginseng C.A. Meyer.) berry has been used as a health-promoting supplements. Also, Mulberries (Morus alba L.) fruit have been used in traditional herbal medicine to treat and prevent diabetes. In this study, we measured the cytotoxicity after fermentation of the extracts in Panax Ginseng Berry and Mulberry Fruit. Methods and Results : The extracts were prepared by decoction for 3 hours in distilled water (100 g/L). The dried extract was then dissolved in phosphate-buffered saline (PBS) in preparation for use. Cell viability was examined by an MTT assay. RAW 264.7 cells were seeded at 1 × 104/mL densities in 96-well plates. Each grouping had a non-treated group as the control. The extracts were added to each well and incubated for 24 hours at 37°C and 5% CO2. The MTT solutions (5 mg/mL) were added to each well, and the cells were cultured for another 2 hours. The supernatant was then discarded, and 100 μL of dimethyl sulfoxide was added to each well. The optical density was read at 540 nm. Conclusion : Probiotics and prebiotics modulate the composition of human and domestic animal gut microbiota. The beneficial effects may result from suppression of harmful microorganisms or stimulation of organisms which contribute in a positive way to the nutrition and health of human and domestic animal. Recently, fermentation using microorganisms for the production of more effective compounds has been extensively studied. In particular, the novel pharmacological effects of a new compound generated by fermentation have been reported. Some previous studies have demonstrated that Fermented herbal medicine extract showed better bioactivity than normal herbal Plants extract when used at the same concentration.

Background : The objective of this study was to Amomum tsao-ko Crevost et Lemarié, (Zingiberaceae) is widely distributed among several countries in South Asia and Southeast Asia. It is a well known spice in Asia, produces a nice refreshing effect in the mouth. Additionally, it's dried fruit is used in Traditional Chinese Medicine (TCM) for cardiac diseases, edema, eye trouble, skin, itch and impotence. The objective of this study was evaluated the inhibitory activity on adipogenesis in 3T3-L1 cells from A. tsao-ko. Methods and Results : The fruits of A. tsao-ko were extracted with 80% EtOH two times at room temperature. The EtOH extract was suspended in distilled water and partitioned with solvent to give CH2Cl2, EtOAc and n-BuOH. The CH2Cl2 was suspended in n-hexane and partitioned with solvent to give 50%, 70% and 90% MeOH. The purification of each fraction by column chromatography separation and HPLC analysis. Consequently, several constituents were isolated five known compounds. The identification and structural elucidation of compounds were established by using NMR (1D and 2D) and mass spectrometry. Conclusion : These compounds were identified as fluorenone (1), phenanthrene (2), anthracene (3), methyl linolenate (4), 1,2-benzenediol (5). All isolates were tested for their inhibitory activities on adipogenesis in 3T3-L1 cells.