The six rowed and naked barley cultivar, “Cheongho” (Hordeum vulgare L.), showing earliness, good cooking quality, resistance to powdery mildew and barley yellow mosaic virus disease (BaYMV), winter hardiness, lodging tolerance, and high yield in paddy fi

A six-rowed naked barley cultivar, Donghanchal (Hordeum vulgare L.), with low-amylose, winter hardiness, viral disease resistance and good quality, was developed from the cross between the advanced line “Masangwamac/Kangbori*7” and Naehanssalbori in 1993.

This study was carried out to investigate spectral irradiance characteristics of blue, yellow, and blue-black colored polyethylene (PE) shading net and the effect on growth characteristics and yield in ginseng seedling. The spectral irradiance (μmol/m2/s/nm) showed the peak at 498 nm in both of blue and blue-black PE shading net, and 606 nm under yellow PE one. The intensity of blue light in blue shading was more strong than that of blue-black shading, control. Blue shading was increased by 17% and 23% in accumulated quantum for daytime, 0.5℃ and 0.2℃ in maximum temperature on June 2 than that of yellow and blue-black shading, respectively, but heat injury ratio of the former was lower than that of the latter. Chlorophyll content and stem length in blue shading were decreased more significantly than those of yellow and blue-black shading. The specific leaf weight was higher under blue and yellow shading than that of blue-black shading. Ginseng seedling harvested in blue shading was increased by 13~17% in the number of root, and 17~20% in root weight per m 2 compared to yellow and blue-black shading owing to the increase of survived plant, and the decrease of specific leaf weight, heat injury ratio, and stem length.

Paeoniae radix has been widely used for its anti-allergic, anti-inflammatory and analgesic effects, and demonstrated to have anticonvulsant, memory enhancing and anxiolytic activities. The present study was performed to examine the protective effect of methanol extract of Paeoniae radix (PR) from Paeoniae Japonica Miyabe et Takeda (Paeoniaceae) on hydrogen peroxide (H2O2)-induced neurotoxicity using cultured rat cerebral cortical neuron. H2O2 produced a concentration-dependent reduction of neuronal viability, PR, over a concentration range of 10 to 100 μg/ml showed concentration-dependent decrease of the H2O2(100 μM)-induced neuronal cell death, as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-di-phenyl-tetrazolium bromide assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. PR (100 μg/ml inhibited 100 μM H2O2-induced elevation of the cytosolic Ca2+ concentration ([Ca2+]c), which was measured by a fluorescent dye, flue-4 AM. PR (50 μg/ml inhibited glutamate release into medium induced by 100 μM H2O2, which was measured by HPLC, and generation of reactive oxygen species (ROS). These results suggest that PR may mitigate the H2O2-induced neurotoxiciy by interfering with the increase of [Ca2+]c, and then inhibiting glutamate release and generation of ROS in cultured neurons.

Dahyang, a new six-rowed and covered barley cultivar, was developed from the cros betwen Milyang26/NICS, RDA in 2005. Among the crosses made in 1994, a promising line that showed both high yield and lodging resistance in theYield Trial tested at Suwon in

Systemic acquired resistance is an important component of the disease resistance repertoire of plants. A novel syntheticchemical, Benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), was shown to induce acquired resistance in wheat.BTH prote

Tissue culture systems to optimize regeneration plant species of Ocimum spp were evaluated as a method to micropropagate individual plants and to better study their biology in vitro. Ocimum species were also evaluated for the production of natural plant products during and following the regeneration process. The primary goal of this project was to enhance the regeneration efficiency of basil. Several factors were examined using different Ocimum species and commercial varieties. The effect of cytokinin combination, activated charcoal, gelling agents, and different carbon sources were investigated. Anthocyanin callus spots were produced only in four varieties among six tested. 'Sweet Dani' showed the best results on anthocyanin accumulation, while 'African beauty', 'Tree basil' and 'Methylcinnamate' produced only a few spots. Shoot regeneration was only achieved from 'Sweet Dani' explants. As the activated charcoal concentration increased, callus formation rate decreased respectively compare to the controls for all varieties. There was a decrease in callus growth with increasing concentration of agar and phytagel.

This study was carried out to develop convenient and reproducible methods for identifying the genetic relationship among germplasms of Panax species based on molecular genetics. Using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses, genetic polymorphism of the Panax species was investigated with following cultivars and accessions, such as Chunpoong, Yunpoong, Kopoong, Sunpoong, and Kumpoong in domestic cultivars, Hwangsuk, Jakyung and Suckju in domestic accessions, and Panax quinquefolius L. and Panax japonicus C.A. Meyer in foreign introduced accessions, respectively. Specific DNA fragments ranging from 200 to 3,000 base pairs in size could be obtained with various ISSR and RAPD primers under the optimized PCR conditions. The dissimilarity coefficients among the genetic polymorphisms of ginseng cultivars and accessions were calculated from 0.26 to 0.90 in RAPD and from 0.12 to 0.89 in ISSR analysis, respectively. Eleven plant samples were grouped siblings together with cultivars and parents based on cluster analysis of genetic distance depending on genetic property such as origin of the species. In results, both RAPD and ISSR analyses were useful for identifying the genetic relationship among cultivars and accessions of Panax species at DNA level.

Sanguisorbae radix (SR) from Sanguisorba officinalis L. (Losaceae) is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of SR on amyloid β Protein(25-35) (Aβ (25-35)), a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. SR, over a concentration range of 10-50 μg/ml, inhibited the Aβ (25-35) (10 μM)-induced neuronal cell death, as assessed by a 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. Pretreatment of SR (50 μg/ml) inhibited 10 μM Aβ (25-35)-induced elevation of cytosolic calcium concentration ([Ca2+]c), which was measured by a fluorescent dye, fluo-4 AM. SR (10 and 50 μg/ml) inhibited glutamate release into medium induced by 10 μM Aβ(25-35), which was measured by HPLC, and generation of reactive oxygen species. These results suggest that SR prevents Aβ (25-35)-induced neuronal cell damage in vitro.

우라늄 변환시설의 라군 슬러지에 함유된 질산염의 안정적 처리를 위해 물 첨가 용해를 실시한 뒤, 여과 케이크의 안정화 특성에 대하여 알아보았다. 물 용해에 의해 대부분의 질산염은 고농도 질산염 용액으로 제거되었으므로, 여과 케이크의 열분해는 에서 하나의 단계로 수행하였다. Muffle furnace를 이용하여 에서 5시간동안 여과 케이크의 열분해를 실시한 결과 라군 1 슬러지에 포함된 U은 의 열분해와 함께 의 형태로 안정화 되었다. 라군 2 열분해 잔류물의 경우에는 열분해 시 생성된 CaO가 냉각과정에서 수분과 반응하여 로 전환되는 것을 TG/DTA 분석과 XRD 분석을 통해 확인할 수 있었지만, 처분장에서 대기중 노출이나 지하수의 침출 등에는 안정한 화합물로 알려져 있으므로, 특별한 첨가제의 첨가 없이 단순 열분해 후 처분이 가능할 것으로 판단된다.