본 연구에서 돼지 난포란에서 채취된 난모 세포들을 체외성숙 후 형태적으로 선별하거나 극체 방출란을 선별하여 활성화 처리 후 48시간째에 분할란을 선별할 때 배발달율이 어느정도 향상되는지를 검토하였다. 난모 세포를 48시간 성숙 배양 후 형태적 선별과 극체의 방출 유무를 검사하고, 선별된 난모 세포들을 시간 추가 배양한 후 7% ethanol로 활성화시키고 cytochalasin B에 5시간 노출 후 PZM-5 배 양액으로 7일간 배양하였으며, 배양 중

This thesis is about John Thomas; the first missionary sent to the KEHC, the first superintendent and the first dean of Kyungsung Bible Institute. The coverage of this thesis begins from his birth in the U.K. and his ministries, how he came to Korea and his ministry in Korea from 1910-1911. In total, John Thomas spent 10 years serving in Korea, therefore assessing his ministry is somewhat out of proportion, however this thesis will focus on the background of his calling to Korea and the beginning of God’s divine works. 1. John Thomas had evident experience in the Fourfold Gospel. 2. His devotion and faith made him a clear model as Christ’s servant. 3. He was a man of prayer. 4. Through the successful ministry in the U.K. he demonstrated the blessings of God’s presence. 5. He made a network of supporters for prayer and financial aid. 6. He was a prominent Evangelical leader of his time and was selected as the main speaker at the annual OMS conference in 1911. 7. He had a call to preach the holiness element. This calling began to spread in the other denominations and missionaries also. 8. He not only loved the lost souls of Korea but also cared for the people of the world. 9. His ministry had a harmony of teaching and ministering. Through his character which was greatly influenced by the holiness element gave him authority to impress many people. 10. Although he had difficulty in learning Korean, his input and effort was vital in setting the foundations of the KEHC and the Bible Institute. 11. The early records of his ministry which influenced many denominations and other missionaries in Korea and Japan shows how influential he must have been in the later years. 12. He contributed to the printing of 10,000 copies of the New Testament, helped set up administrative procedures for KEHC and the Bible Institute and his teaching, church founding and faithful mission works show the bright future ahead. This research focuses on the early days of John Thomas’s mission. There are still more research to be done about his character and the latter part of his ministry. However, the fact that a man of God, such as John Thomas was called to serve as the first missionary sent to the KEHC, the first superintendent and the first dean of Kyungsung Bible Institute proves God’s purpose toward the KEHC and STU. Furthermore, through this research we need to find the elements that need to be continued and carried out in this day and age. Therefore it is inevitable the existence of holiness is revealed not only in Korea but also worldwide. This is the core value of KEHC and STU’s existence.

본 논문에서는 일차전단변형이론(FSDT)을 이용한 복합재료 적층평판의 고정밀 해석기법을 소개한다. 전단수정계수가 자동적으로 포함되도록 횡방향 전단 변형에너지를 혼합변분이론(mixed variational theorem)을 이용하여 개선하였다. 혼합변분이론에서는 변분을 횡방향 응력들에 대해서만 취하였다. 가정된 횡방향 전단응력은 효율적인 고차이론(Cho and Parmerter, 1993)으로부터 구하였다 횡방향 수직응력은 3차 다항식으로 가정하였고, 무전단 응력조건과 평판의 윗면과 아랫면에서의 응력을 만족하는 조건을 부과함으로써 얻었다. 한편, 변위들에 대해서는 일차전단변형이론의 변위장을 사용하였다. 이렇게 해서 얻어진 변형 에너지를 본 논문에서는 EFSDTM3D이라고 명명 하였다. 본 논문에서 개발된 EFSDTM3D는 변위와 응력의 계산에서 고전적인 FSDT와 같은 정도의 계산 효율을 가지면서, 동시에 변위와 응력의 두께방향의 정확도를 면내 방향 응력들에 대한 최소오차자승법에 기초하여 응력 회복 과정을 적용함으로써 개선하였다. 계산된 결과는 고전적인 FSDT, 3차원 탄성해, 그리고 참고문헌 중에서 이용 가능한 결과들과 비교하여 검증하였다.

Grounded in the positioning theory and the notion of transaction, this study examined two first grade English language learners in the ESL classroom in a North American elementary school. The research aimed at exploring 1) how the two children participated in the ESL classroom and developed positioning, and 2) what the patterns and paths of the students’ positioning told about their learning English. Observation of the two children in the classroom and extensive field notes were the main data for the study. The analysis of the data revealed contrasting paths of positioning the two children took up in the process of making transaction with the teacher and the classroom lesson acts. The study has several suggestions for researchers, educators, and parents.

Cyclin B1 is known to reflect the M-phase promoting factor (MPF), a universal regulator of G2/M-phase transition, activity during the process of oocytes maturation. To investigate whether culture condition affects the maturation rate and the expression of cyclin B1 protein, bovine immature oocytes are stimulated and cultured according to the following protocols: Experiment 1: denuded oocytes (denude) only, COC only, denuded oocytes + granulosa cells (denude + GCs) and COC + GCs; Experiment 2: no-activation (control), 7% ethanol for 5 min and 10 l/ml ionomycin for 5 min at immediately before maturation. The maturation rates of denude and no-activation group were significantly lower in both experiments (P<0.05), respectively. Co-culture or stimulation method in bovine immature oocytes culture increases the cyclin B1 expression significantly in both experiments (P<0.05). Based on these results, culture condition affects the maturation rate and the expression of cyclin B1 protein during the first meiotic maturation in bovine immature oocytes.

Two morphologically different species of Peridinium were frequently observed in natural samples collected monthly from Togyo Reservoir. One was previously identified as P. bipes Stein f. occultatum (Lindem.) Lef. (Ki and Han 2005), the other was remained