Differentiation of Pleurotus eryngii is laborious and time-consuming tasks especially in mycelial status. For development of a method for differentiation of P. eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotypes. PCR with the each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pair-group method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into 2 clusters. Cluster I and II comprised of 4 and 8 cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivar and strains with 21 allele with a PIC value of 0.9090. These results might be useful to provide an efficient method for the identification of P. eryngii cultivars with separate PCR reactions. (This work was supported by a grant from the Gold Seed Project [Supported by a grant from the IPET (213003-04-3-WTI11), MIFAFF, Republic of Korea.]

For research and development of Silicon Carbide (SiC) mirrors, the Korea Astronomy and Space Science Institute (KASI) and National Optical Astronomy Observatory (NOAO) have agreed to cooperate and share on polishing and measuring facilities, experience and human resources for two years (2014-2015). The main goals of the SiC mirror polishing are to achieve optical surface figures of less than 20 nm rms and optical surface roughness of less than 2 nm rms. In addition, Green Optics Co., Ltd (GO) has been interested in the SiC polishing and joined the partnership with KASI. KASI will be involved in the development of the SiC polishing and the optical surface measurement using three dierent kinds of SiC materials and manufacturing processes (POCOTM, CoorsTekTM and SSGTM corporations) provided by NOAO. GO will polish the SiC substrate within requirements. Additionally, the requirements of the optical surface imperfections are given as: less than 40 um scratch and 500 um dig. In this paper, we introduce the international collaboration and interim results for SiC mirror polishing and development.

The present study explored the effects of different task types on the autonomous vocabulary learning of EFL learners. To this end, two types of tasks with similar but slightly different levels of involvement loads were developed: a corpus-based task and a word map task. The participants of the study were 41 EFL students from a university in Seoul, Korea. They were divided into two groups: a corpus group (n=20) and a word map group (n=21). A total of 33 target words were selected and the experiment was administered during a period of five weeks. Major findings from the study were as follows: First, the corpus task proved to be more effective than the word map task in productive vocabulary learning, whereas the two tasks showed similar effectiveness in receptive vocabulary learning. Second, the two tasks varied in the effectiveness of vocabulary learning depending on the learners' vocabulary proficiency levels and the specific domains of vocabulary knowledge (receptive and productive). Lastly, the corpus group responded more positively than the word map group to their task and to the vocabulary learning. The findings indicate that the corpus task has advantages over the word map task and provide ‘residual’ empirical support for Hulstijn and Laufer’s (2001) involvement load hypothesis. Based on the findings, some pedagogical implications were made for the effective teaching and the autonomous learning of vocabulary.

This study analyzed the growth characteristics of multi-layer planted trees through their growth analysis and attempted to present a management strategy. The subject of research is the Citizen's Forest Area of Seoul Forest Park located in Seoul City. Field surveys were conducted three times over eight years from 2005 when the Seoul Forest Park was created through 2013. Labels were attached to all trees in the target area, and their species, height and DBH were investigated. To identify the growth differences by trees in each area, a detailed tree location map was drawn up for use in the analysis. To check soil health, soil organic matter, soil pH and soil microbial activities were analyzed. It turned out that the growth of the multi-layer planted trees in the target area of research was higher than that of the trees in existing urban parks, and that it was similar to that of trees in natural forests. Through a field survey in the area with a remarkably low growth, high-density planting problem, soil was found to have excess-moisture and there was the problem of Pueraria lobata covering. As a result of the analysis of the soil, it was found that its organic content in the soil was lower; soil pH was higher; and microbial activities in the soil were lower when compared to that of natural forests.

This study is intended to evaluate the value of functional aspect from the viewpoint of habitat. The indicators that are used in biotope evaluations are various, but most of them use the criteria to evaluate the naturality. This evaluation method cannot appropriately reflect the functional characteristics coming from relation to the surrounding biotope. In this study, the connectivity, cohesion and diversity between individual biotope are quantitatively measured by a landscape index. It is hard to draw the functional value of individual biotopes because the landscape index related to connectivity, cohesion and diversity comes from a landscape having a number of biotopes. The concept of contribution was used to overcome this limitation. The concept of contribution is to quantify how much each individual biotope contributes to the connectivity, cohesion, and diversity in a certain range of landscape by deriving the amount of change in the landscape index according to the presence or absence of each individual biotope. In order to understand the characteristics of evaluation results in functional aspect, this research has done a comparative analysis of the previous research findings in the same target area. According to the result of the research, individual biotopes such as artificial forests, fragmented natural forests, and small planting sites were highly rated.

The master plan of forest land management proposes forest watershed management that considers regional characteristics in order to overcome the problem of uniform forest land management. In order to manage the forest watersheds in Gyeonggi-do, this study classified 1,823 forest watersheds in Gyeonggi-do and attempted to understand their characteristics. It conducted a factor analysis and cluster analysis from the perspective of conservation value and development pressure using forest land indicators. In terms of conservation value, three factors were drawn: the topography factor, vegetation factor and public service factor, while in terms of development pressure, three factors were drawn: the easiness of development factor, economic benefits factor and development activity factor. Using these factors, forest watersheds were divided into three clusters in terms of conservation value while they were divided into three clusters in terms of development pressure. Using the results of the cluster analysis from a conservation-development perspective, the forest watersheds were classified into nine different types, and the characteristics were identified by each type. It is judged that the factors and clusters drawn as a result of the research accurately reflect the present conditions of Gyeonggi-do, and the nine types of forest watersheds have clear characteristics according to each type, which are judged to be utilized in forest management in the future.

Distribution and abundance of species are influenced by their environmental condition. Among various environmental factors, a vegetation is one of the most influential factors, because it is used as habitats as well as food resources. Arthropods including insects play a important role in forest ecosystems as grazers, scavenger, predators, etc. Deciduous and coniferous forests have different plant community composition, resulting in different environmental condition. In this study, we collected arthropod communities at two different deciduous and coniferous forest stands in the Mountain Gariwang. At each stand, three different sampling methods were used: soil core for soil macroinvertebrate, pitfall trap for wandering insects, and sweeping net for insects in the shrups. Therefore, sampling was conducted at vertical habitats in the forest. The results showed that there were no difference in the number of orders from soil at both coniferous and deciduous stands. However, the abundance was higher at coniferous stand than at deciduous stand. Collembola was the dominant taxa in soil. Meanwhile, there were no statistically significant differences of community composition between coniferous stand and deciduous stands. Multivariate analyses was conducted to characterize differences of communities at different stands as well as vertical distribution in forest.

This study aimed to examine the most effective exercise method for shoulder joint rehabilitation by comparatively observing activity of shoulder stabilizer muscles during push up and push up plus exercises under diverse stable conditions. The subjects were 20 healthy adults students who went to M university. While the subjects conducted push up and push up plus exercises under diverse stable conditions(a stable surface, a support of 25cm height, a support of 30 cm height and a balance pad), activities of the upper trapezius muscle, pectoralis major muscle, serratus anterior muscle, and triceps brachii muscle were recorded. During push up and push up plus exercises, activities of the stabilizer muscles were higher when the stable condition was changed rather than on the stable surface. In particular, when the support of 30cm height and balance pads were applied, activity of the shoulder stabilizer was highest. There were significant differences in the upper trapezius muscle and triceps brachii muscle during the push up exercise(p<.05) and in the serratus anterior muscle during the push up plus exercise(p<.05). Activities of the shoulder stabilizers were higher when the upper and lower limbs' surface stable conditions were changed than the stable surface. Therefore, when programs for rehabilitation of shoulder joints are applied, provision of diverse stable conditions according to patients' conditions will be effective methods.

In the present study, we investigated the effect of staurosporine on the formation of cellular processes in human gingival fibroblasts and rat astrocytes. Staurosporine caused a rapid induction of process formation in human gingival fibroblasts and rat astrocytes in a concentration dependent manner. The process formation of human gingival fibroblasts and rat astrocytes was prevented by the pretreatment with N-acetylcysteine, suggesting that staurosporine-induced ROS production was responsible for the process formation. Colchicine, a microtubule depolymerizing agent, inhibited the staurosporine-induced process formation, whereas cytochalasin D, an actin filament breakdown agent, failed to suppress the formation of cellular processes. This result indicated that polymerization of microtubule, and not actin filament, was responsible for the formation of cellular processes induced by staurosporine. In support of this hypothesis, Western blot analysis was conducted using anti-tubulin antibody, and the results showed that the amount of polymerized microtubule was increased by the treatment with staurosporine while that of depolymerized beta-tubulin in soluble fraction was decreased. These results indicate that staurosporine induces ROSmediated, microtubule-dependent formation of cellular processes in human gingival fibroblasts and rat astrocytes.

To examine the relationship between competition and environmental factors, the germlings of Ascophyllum nodosum (L) Le Jolis and Fucus vesiculosus L. were cultured in monocultures and mixtures of the two species under two different exposure and nutrient levels. Both intra- and inter-specific competition were examined in comparison of the mortality and growth of germlings in monocultures and mixtures of the two species. The mortality of germlings increased with increasing density and emergence periods both in the monoculture and mixtures of the two species, and the mortality of Ascophyllum was significantly higher than that of Fucus both in submerged and emerged treatments. The growth of germlings of both species reduced with increasing density but F. vesiculosus always grew faster than Ascophyllum. The values of log output ratio were more than 0.1, indicating that Fucus 'won' in the competitive battles with Ascophyllum under two nutrient- and air-exposure levels. Log output ratio was greater in high than in low nutrients, indicating that the growth of Fucus is more enhanced than that of Ascophyllum in high nutrients. In the present study, the outcome of inter specific competition between germlings of Fucus vesiculosus and Ascophyllum nodosum was slightly altered by duration of emergence and nutrient concentration, but not to such an extent as to change the outcome.

최근 10년간(2004 2013년) 국내의 조선소에서 발생한 해양오염사고의 건수를 연도별, 지역별, 조선소 작업별 및 오염물질별로 분석하였고, 이 분석 결과를 바탕으로 조선소 내 오염사고 방지 방안을 제시하였다. 전국의 조선소 내 오염사고는 10년간 총 103건이 발생하여 연평균 10.3건이었고, 연간 발생건수는 2004년 8건에서부터 점증하는 추세를 보이면서 2010년 23건으로 최고를 기록하였다가 다시 감소하는 추세로 바뀌어 2014년 9건을 나타내었다. 지역별 오염사고 건수는 부산이 32건(31%)으로 1위이고, 전남이 30건(29%), 경남이 21건(21%), 제주가 5건(5%), 강원과 경북이 각각 4건(4%), 인천과 충남이 각각 3건(3%)이었다. 조선소 작업별 오염사고 건수는 선박수리 작업이 60건(58%)으로 1위이고, 선박해체 작업이 25건(24%), 선박건조 작업이 10건(10%), 기타가 8건(8%)이었다. 오염물질별 오염사고 건수는 기름 및 유성혼합물이 59건(57%)으로 1위이고, 폐페인트 가루가 22건(21%), 쇳가루 및 용접슬래그가 13건(13%), 폐기물이 4건(4%), 폐FRP 가루가 3건(3%), 기타가 2건(2%)이었다. 국내 조선소 내의 오염사고를 방지하기 위한 방안으로는 ① 관련 법규의 준수, ② 조선소 밀집 지역에 대한 오염사고방지 실행계획의 수립 및 시행, ③ 특히 선박 수리 및 해체 작업을 하는 경우 조선소 내 기름오염방지 방안의 수립 및 시행, ④ 조선소 오염사고에 대한 민원 해결 대책의 마련, ⑤ 조선소 단속에 대한 국가 관리의 개선 등을 제시하였다.

The inhibitory activities of the Cordyceps pruinosa butanol fraction (Cp-BF) were investigated by determining inflammatory responses of lipopolysaccharide (LPS)-treated RAW264.7 macrophage cells and by evaluating HCl/ethanol (EtOH)-triggered gastric ulcers in mice. The molecular mechanisms of the inhibitory effects of Cp-BF were investigated by identifying target enzymes using biochemical and molecular biological approaches. Cp-BF strongly inhibited the production of NO and TNF-α, release of reactive oxygen species (ROS), phagocytic uptake of FITC-dextran, and mRNA expression levels of interleukin (IL)-6, inducible NO synthase (iNOS), and tumour necrosis factor-alpha (TNF)-α in activated RAW264.7 cells. Cp-BF also strongly down regulated the NF-κB pathway by suppressing IKKβ according to luciferase reporter assays and immunoblot analysis. Furthermore, Cp-BF blocked both increased levels of NF-κB-mediated luciferase activities and phosphorylation of p65/p50 observed by IKKβ overexpression. Finally, orally administered Cp-BF was found to attenuate gastric ulcer and block the phosphorylation of IκBα induced by HCl/EtOH. Therefore, these results suggest that the anti-inflammatory activity of Cp-BF may be mediated by suppression of IKKα and its downstream NF-κB activation. Since our group has established the mass cultivation conditions by developing culture conditions for Cordyceps pruinosa, the information presented in this study may be useful for developing new anti-inflammatory agents.