The Eurytoma appendigaster species-group is a second large group within the genus Eurytoma Illiger found in the Palearctic region. This group comprises 44 species by Zerova, but in Korea, only one species, E. appendigaster (Swederus), has been previously reported. They are well-known for their predation on phytophagous insects, mainly the genus Tetramesa which is belonged to the family Eurytomidae and associated with grasses (Poaceae). In this study, we report seven species of the appendigaster species-group from Korea for the first time. Also, key to Korean species of appendigaster species-group and photos of diagnostic characteristics are provided.

The genus Margarinotus Marseul, 1854 is one of the highly diverse groups of Histeridae (Histerinae), consisting more than 100 described species in the world. About 80 species are known to distributed in Palaearctic region, among them, 11 species have been reported from Korea. In this study, we report a new species, Margarinotus pseudoboleti sp. nov. and a new record, Margarinotus yezoensis Ôhara from Korea. Also, we provide a revised taxonomic key for Korean Margarinotus, including above two species and images of adults.

Spodoptera litura Fabricius, 1775 (Lepidoptera: Noctuidae) is a serious crop pest with a long-distance migratory flight. To date, the DNA barcode region has been widely used in genetic diversity analysis studies of Spodoptera litura. However, the DNA barcode region showed maximum variation rate of S. litura, which from 18 regions in South Korea, was 0.608% (nine haplotypes) in previous study. In this study, four mitochondrial genes (ND4, ND4L, ND1, 16s rRNA) have higher intra-specific variation rates than the DNA barcode region. Among the four genes, The variation rate of the 16s rRNA region was confirmed to be a minimum of 0.203% (2bp) and a maximum of 1.824% (18bp). Finally, the 16s rRNA region with the highest PCR amplification efficiency and highest variation rate was selected as a high-efficiency molecular marker.

The genus Norbanus Walker 1843 (Pteromalidae: Pteromalinae) consists of 38 species distributed worldwide. Among them, 13 species have been described from the West and Central Palaearctic (Noyes 2003). Norbanus is one of several pteromalid genera that shares the character of a narrow spicula on the clava. In Korea, only one species in the genus Norbanus is recorded. In this study, we report Norbanus meridionalis for the first time in South Koera. We provided description and diagonostic illustration of Norbanus meridionalis.

The yellow mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae), is an important industrial insect commercially produced around the world as food and feed. Temperature and nutrition are the two most influential environmental factors determining the rearing conditions in insects, but little is known about how these two factors interact to affect the performance of T. molitor larvae. In this study, we investigated the combined effects of temperature and dietary protein:carbohydrate (P:C) ratio on key performance traits in T. moltior larvae. Throughout their larval stage, the insects were reared on one of 36 treatment combinations of six temperatures (19, 22, 25, 28, 31, 34 °C) and six protein:carbohydrate ratios (P:C = 1:5, 1:2, 1:1, 2:1, 5:1, 1:0) and their survivorship, development, growth rate, and pupal mass were monitored. Survivorship was high at low temperatures (< 25°C) and high P:C ratios (>1:1), but decreased with increasing temperature and decreasing P:C ratio. Increase in rearing temperature accelerated larval development but resulted in a reduced pupal mass. Thermal optimum for pupal mass (19.3°C) was thus lower than that for development time (28.1°C). The growth rate was maximized at 27.9°C and P:C 1.65:1 and decreased as both the temperature and the P:C ratio deviated from their optimum. All four key performance traits (survivorship, development time, pupal mass, growth rate) were optimized at temperatures between 25.7 and 27.4°C and P:C ratios between 1.17:1 and 2.94:1. Our data provide insights into how the production and nutritional value of T. molitor larvae can be improved through adjusting their rearing conditions.

Stilbene-based fluorescent brighteners (FB) have been shown to enhance insecticidal activities of entomopathogenic viruses but little is known its effect on entomopathogenic bacteria. We investigated the effect of two FBs (FB 28 and FB 71) on the insecticidal activity of B. thuringiensis var. kurstaki (Btk) as well as the Lymantria dispar multiple nuclear polyhedrosis virus (LdMNPV) in Lymantria dispar asiatica. FB 28 increased the mortality at the combination with low concentration (1.6×102 IU/ml) of Btk, but FB 71 slightly reduced the mortality with middle and high concentrations (1.6×103 and 1.6×104 IU/ml) of Btk in comparison to Btk alone. Both FB 28 and FB 71 increased mortality in combination with LdMNPV at all concentrations (3×102, 3×104, and 3×106 POBs/ml) compared to LdMNPV alone. Our findings suggest that FBs enhanced pathogenic activities but depend on chemical nature of FBs.

The striped fruit fly (SFF), Zeugodacus scutellata, is an agricultural pest species with a strong and rapid reproductive ability that can cause significant harm. To control the population of these kind of pests, the sterile insect technique (SIT) is being used as one of the effective methods. SIT involves the introduction of sexually transmitted factors that reduce the reproductive capacity of males. This study shows that knocking down the testis-specific serine/threonine protein kinase 1 (Zs-Tssk1) gene alters male fertility and male-initiated types of communication. Since Zs-Tssk1 influences the physiology of the testes, spermatogenesis is also affected, which in turn alters the lifespan of Zs-Tssk1 knock down group in comparison with the control. Based on these results, Zs-Tssk1 may be crucial in reproductive function, and its down-regulation may be helpful in controlling SFF through SIT.

초파리를 당뇨병을 연구하는데 사용하는 경우가 많으나 당뇨병이 유전적 요인에 작용하여 다음 세대에 미치 는 영향을 다루고 있는 연구는 적다. 따라서 본 연구에서는 초파리에 고당도의 먹이를 통해 당뇨병을 유발하고, 류신(leucine)의 당뇨병에 대한 치료효과를 확인하고 다음 세대에 미치는 영향을 알아보고자 했다. 본 연구에서 는 Drosophila melanogaster를 초파리 모델에 사용했다. 먹이에 고농도의 설탕, 고농도의 설탕과 류신을 첨가하여 당뇨의 발병과 당뇨병의 치료효과를 확인했다. 당뇨병의 발병을 확인하기 위해 초파리의 체내의 포도당을 측정 하여 대조군에 비해서 고농도의 설탕을 섭취한 경우에 포도당의 농도는 증가했고, 류신을 섭취한 경우에는 포도 당이 대조군에 비해서도 급격히 떨어지는 것을 확인했다. 고농도의 설탕이 포함된 먹이 조건을 유지하고 교배를 통해 자손을 얻고자 하였다. 그 결과, 고농도의 설탕을 섭취한 경우 자손의 몸의 크기가 0.1mm 정도 감소하고 무게 또한 감소했다. 그러나 고농도의 설탕과 류신을 섭취한 경우 자손을 얻지 못했다. 당뇨병을 유발시킨 초파리 를 이용해 류신과 운동의 효과를 동시에 확인했다. 당뇨병이 유발된 경우 운동만으로는 포도당의 감소에 영향을 주지 못하였으나, 류신 운동을 병행한 경우에는 포도당이 감소했고 수컷 초파리에서 잘 관찰되었다. 운동과 류신 섭취를 병행하는 실험에서는 당뇨병을 유발한 초파리의 자손을 사용했기 때문에 크기는 당뇨병을 유발하지 않은 것보다 작았다. 따라서 초파리의 당뇨병 모델을 통해서 당뇨병이 유전적으로 전달되며 Leucine에 의한 치료 는 성충에 수행되는 것이 효과적임을 알 수 있었다.

In Korea, the Asian honey bee (Apis cerana) and the European honey bee (Apis mellifera) (Hymenoptera: Apidae) are the two most common honey bee species. These two closely related species are known to have different sensitivity levels to various insecticides due to millennia of exposure to different pests and pesticides. It is reported that A. cerana is known to be more sensitive to several insecticides, such as amitraz, fenitrothion, and fipronil, than A. melllifera. Multiple studies investigated toxicological responses and related CYPome in A. mellifera, but little is known in A. cerana. The goal of this study is to elucidate the underlying mechanism of different toxicological responses between two bee species, with an emphasis on cytochrome P450 (P450), a significant enzyme involved in metabolic activities. The differences in basal P450 expression patterns were investigated by comparing the relative expression levels of P450 orthologs in several dissected organisms of each species. To compare the sensitivity against major insecticides, lethal doses of major insecticides relevant to both honey bee species were assessed by topical and oral ingestion bioassays. The determined sublethal doses of insecticides were applied to honey bees, and the inducibility of P450s was investigated by comparing the expression patterns of multiple P450s. From these results, this study eventually attempts to compare the toxicological differences between two Apis species with differences in induced cytochrome P450 expression levels.

Riptortus pedestris has established symbiotic interactions with specific bacteria from soil environment in every generations. The soil environment is extremely heterogeneous with microbial diversity, suggesting that bacterial composition of the R. pedestris can be affected by surrounding environments. Therefore, we investigated spatiotemporal variation of bacterial communities in wild R. pedestris collected from five mainland areas and Jeju Island in South Korea across insect generations. Among the 390 R. pedestris adults collected, we detected a total of 28 bacterial genera from the symbiotic region of insects. Among the bacterial genera, genus Caballeronia bacteria was the most abundantly detected regardless of sampling site and generation. Nevertheless, bacterial richness varied among the insect generations with 10 genera detected in overwintered generation, 15 genera in the 1st generation and 18 genera in the 2nd generation. Moreover, we found five bacterial genera that were exclusively detected from the Jeju island compared to the mainland areas surveyed.

The Varroa mite, Varroa destructor, a parasitic mite that afflicts honey bees, has become increasingly resistant to acaricides like fluvalinate due to its widespread use. The target site insensitivity mechanism, mediated by the L925V/M/I mutations in the voltage-gated sodium channel, plays a major role in resistance. Additionally, cytochrome P450 monooxygenases (Cyp450s) appear to function as a metabolic resistance factor; however, no Cyp450-mediated resistance mechanism has been reported to date. The aim of this study was to identify and characterize Cyp450s associated with fluvalinate resistance. A synergistic bioassay confirmed the involvement of Cyp450s in conferring tolerance or resistance to fluvalinate. Correlation analysis between mortality data and the expression levels of Cyp450 genes led to the identification of several candidates that may play a crucial role in fluvalinate resistance. Analysis of tissue distribution patterns revealed that these genes were most abundantly expressed in the cuticle and synganglion. This suggests that, despite their relatively low expression level, they may play a critical role in protecting the target site from fluvalinate due to its predominant expression in neuronal tissues. Functional analysis, in conjunction with baculovirus expression, demonstrated that fluvalinate has high inhibition rates against the recombinant candidate Cyp450s, suggestive of their strong interaction with fluvalinate. We discussed the potential utilization of their expression levels as a molecular marker for diagnosing metabolic resistance in field-collected Varroa mites.

The box tree moth, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae), is a native species in East Asia specialized in box trees (Buxus sp.) but has recently invaded most European countries. Due to the absence of its natural enemies in Europe, the economic impact is serious in most countries. In order to find parasitoids as biological control agents in Korea, C. perspectalis larvae were collected from ornamental and native box trees from various regions of the country from 2022 to 2023. While rearing them in the insectary, several parasitoids emerged from the larvae. We found at least six larval parasitoids, including five wasp species and one fly species. Molecular diagnosis using the nucleotide sequences of the mitochondrial cytochrome oxidase subunit I (COI) showed that most samples were 90–97% identical to the data in the NCBI database. Further studies are required to determine biological and parasitic characteristics, such as developmental rate, fecundity, host specificity, and parasitic rate, to clarify the suitability of parasitoids as biological control agents.

Historically, the control of stored-product insects has mostly relied on the use of fumigants such as methyl bromide (MB) and phosphine. However, methyl bromide is no longer used for structural fumigations, and phosphine insecticide resistance is rising globally. Methyl benzoate (MBe) is a new green pesticide that occurs naturally as a metabolite in plants. In this study, we evaluated the the potential use of MBe as a fumigant against a variety of stored-product insects. According to our laboratory findings, MBe showed strong fumigation toxicity against the Indian meal moth and flat grain beetle with an LC50 value of 0.1 μL/L and 0.76 μL/1.5 L air, respectively, compared to the other tested insects. Furthermore, no significant differences were observed in susceptibility levels between the lab strain and the phosphine-resistant lesser grain borer and rice weevil. However, the red flour beetle had the highest LC50 value of 8.26 μL/1.5 L air. Overall, MBe seems to be a highly promising candidate for the development of environmentally-friendly alternative fumigants.

The aphid genus Tuberaphis Takahashi belongs to the tribe Cerataphdini (Hemiptera: Hormaphidinae) and comprises 17 valid species. Generally, its species are distributed in East and South East Asia, where they primarily feed on Styrax spp. (Styracaceae) and utilize species within the Loranthaceae family as their secondary hosts. They form various shapes of galls on Styrax, looking coral and broccoli etc. Currently, the only aphid species reported to exclusively produce gall forms resembling broccoli head-shaped is Tuberaphis takeouchii (Takahashi). For the first time in Korea, an aphid species forming gall structures with a broccoli-head-like appearance has been discovered. We confirmed that the species discovered in Korea is a new species through morphological and molecular comparisons with T. takenouchii (Takahashi). To comprehend the life cycle of this new species, we conducted a year-long study investigating how the gall forms change and examined aphid polymorphism across different seasons. The results of this study additionally include as followings: 1) Species identification, 2) DNA barcoding, 3) population genetic analyses, and 4) morphological comparison using SEM

Miridae show remarkable diversity (>11,700 spp.), accounting for a quarter of all Heteroptera. However, their phylogeny and evolutional history is still remain unclear. In this work, we provide new suggestions for the phylogeny of Miridae using a larger dataset than previous studies. In addition, we suggest an alternative evolutionary history based on newly calibrated divergence dates for Miridae and its subordinate groups, and present probable factors of the family’s success in terms of species diversity. The entire dataset comprises 16 outgroups and 188 ingroup taxa including all seven known subfamilies and 37 out of 45 known tribes. Each species is aligned as 3,577 bp with six molecular loci (COI, 16S rRNA, 18S rRNA, 28S rRNA D3 region, H2A, and H3A).

Nutrient acquisition by insect herbivores affect all aspect of the the lifespan of individauls. For seed-sucking insect herbivores, they face challenges with nutrient acquisition due to requirement for extra-oral digestion of seed contents into a readily-ingestible state. In this study, we demonstrated environmentally-transmitted Caballeronia insecticola allow seed-sucking R. pedestris to overcome challenges with extra-oral digestion. Through the evaluation, first, we found symbiotic insects exhibited enhanced feeding efficiency by consuming significantly larger amount of food per feeding attempt compared to apo-symbiotic insects (P<0.05). Then, we observed feeding behavior modification in the symbiotic insects from the behavior tracking evaluation. Symbiotic insects displayed dichotomic behavior which can be generally divided into early focused feeding and later subdued resting periods. By contrast, apo-symbiotic insects displayed unordered behavior by frequent switches between feeding and walking behavior.

사막이리응애(Neoseiulus californicus)는 칠레이리응애에 비하여 고온건조한 조건에서 유리하고 점박이응 애, 차응애, 차먼지응애 등 응애류 뿐만 아니라 가루이, 총채벌레 등 다양한 미소해충을 포식 포식하는 광식성 천적으로서 전세계적으로 산업화되고 있다. 사막이리응애를 농가수준에서 증식하기 위하여 간단한 대량생산 방법을 개발하였다. 천적의 먹이원인 가는다리고기진드기와 배지로서 밀기울(70%) 및 팽창질석(30%)을 멸균 팩에 넣고 사막이리응애를 사육하였다. 그 결과 사막이리응애의 밀도는 3주차에 18배로 증식되었다. 증식된 사막이리응애를 딸기 농장에 2월초부터 4월초까지 8주간 매주 투여한 결과 잎당 점박이응애의 밀도는 무처리구 에서 41.5마리이고 천적처리구는 7.0마리로서 점박이응애 방제효과가 83%로 나타났다. 본 연구를 통하여 자가 생산한 사막이리응애를 활용하여 딸기농가의 점박이응애 방제에 효과적으로 사용할 수 있을 것으로 판단된다.

경북 안동의 진걸맘농장은 딸기와 쪽 농사를 주로 하고 있으며 10년 이상 친환경 무농약 농법을 인증받은 농가로서 천적을 활용하여 토경 1,000평 규모의 시설 하우스에 딸기 재배를 하고 있다. 2018-2020에 경북농민사관학교 친환경 천적과정을 통하여 천적의 자가생산 기술을 배우고 2021년부터 농가에서 직접 자가생산을 시작하였다. 그래서, 진걸맘농가는 칠레이리응애를 자가사육하여 지속적으로 딸기 재배지에 처리함으로서 점박이응애 방제에 성공한 농업인으로서 국내 첫 사례로 꼽힌다. 또한, 2022년 안동시농업기술센터에서 안전농산물 생산을 위한 천적 배양시 스템 보급 시범 사업에 선정되어 딸기 해충의 생물적 방제를 위한 뿌리이리응애, 칠레이리응애, 보리뱅커플랜트 등의 천적 배양시설 완공, 천적의 자가생산 및 성공적인 현장적용 기술로서 농산물의 지역특화, 브랜드화를 통한 농산물 가격 차별화에 크기 기여하였으며 국내 천적농업의 발전을 위해 앞장서고 있는 선도농가로 인정받고 있다.

경북대학교 친환경농업연구센터에서는 농업인들의 효과적인 천적 활용을 증진시키기 위하여 2016년부터 경상북도 농민사관학교의 지원을 받아서 친환경천적 교육과정을 개설하고 천적 자가생산 및 현장적용 기술의 실습교육을 실시하였다. 그동안 토양 포식성 천적인 총채가시응애(Gaeolaelaps aculeifer)와 뿌리이리응애 (Stratiolaelaps scimitus), 그리고 점박이응애의 포식성 천적인 칠레이리응애(Phytoseiulus persimilis)와 사막 이리응애(Neoseiulus californicus)의 간편하고 경제적인 대량생산기술을 개발하고 그 기술을 약 180명의 농업 인에게 전수하였다. 즉, 농업인들이 스스로 천적을 자가생산하여 시설재배지에 적용함으로서 비용절감 및 청정 농산물 생산 효과를 높히는데 기여했다. 또한, 간편한 천적생산기술을 전국적으로 농업기술원, 농업기술센터, 그리고 친환경농업인단체 등 20여개 기관에 전수하였다. 최근, 경북의 농업기술센터(안동, 고령, 경주)에서 농업 인을 대상으로 천적 대량생산 시스템 보급 시범 사업을 실시하면서 뿌리이리응애 및 칠레이리응애 등의 천적 자가생산시설을 구축하고 자체적으로 생산함으로서 지속적인 천적 활용이 가능하게 되고 해충 방제 효과를 증가시킬 수 있었다. 즉, 농업인들이 직접 천적을 생산하고 활용하게 함으로서 천적농업의 성공사례가 증가하였 을 뿐만 아니라 국내 천적 공급 시스템이 다양화되어 천적농업의 활성화에 크게 기여한다고 판단한다.

Honey bees are crucial pollinators for agricultural and natural ecosystems, but are experiencing heavy mortality in Korea due to a complex suite of factors. Extreme winter losses of honey bee colonies are a major threat to beekeeping but the combinations of factors underlying colony loss remain debatable. Finding solutions involves knowing the factors associated with high loss rates. To investigate whether loss rates are related to Varroa control and climate condition, we surveyed beekeepers in korea after wintering (2021–2022 to 2022–2023). The results show an average colony loss rate of 46%(2022) and 17%(2023), but over 40% colony loss before wintering at 2022. Beekeepers attempt to manage their honey bee colonies in ways that optimize colony health. Disentangling the impact of management from other variables affecting colony health is complicated by the diversity of practices used and difficulties handling typically complex and incomplete observational datasets. We propose a method to 1) Varroa mite population Control by several methods , and 2) Many nursing bee put in hive before wintering.