The vector mosquito surveillance is important when recommending prevention and control measures. The surveillance of the vectors were conducted at a total of 7 locations with light emitting diode (LED traps) and BG-Sentinel traps from March to November, 2023 in Gyeongnam province. In the surveillance, a total of 13 species belonging to seven genera were collected in a total of 6,537 females and 51.9/trap/night (Trap Index; TI) in 2023. Among the collection sites, Gangseo-gu at the pigsty in Busan with a LED trap recorded the highest TI 60.6 (Species Ratio; SR 65.6%) for Culex tritaeniorhynchus females in 2023. Each one of Cx. tritaeniorhynchus female was firstly collected for 2023 at Eulsukdo in Busan at 22th March. In the results of isolation of viral RNA, Flavivirus were positive in one pool (Chaoyang virus; 30 individuals) of Aedes vexans and negative in 341 pools (4,152 individuals) of 12 mosquito species including Cx. tritaeniorhynchus, Cx. pipiens, Aedes vexans, Ae. albopictus, and Ochlerotatus togoi in 2023.

A tick survey was conducted to monitor ticks using tick traps attached dry ice method at each four sites in Ulju and Gimhae counties, Gyeongsangnam-do from April to November, 2023. Two species belonging to one genera were collected with tick traps. A total of 1,064 ticks were collected as Haemaphysalis longicornis (Trap Index; TI 11.0), Haemaphysalis flava (TI <0.1) in Ulju and A total of 843 ticks were collected as Haemaphysalis longicornis (Trap Index; TI 8.7), Haemaphysalis flava (TI 0.1) in Gimhae 2023. Haemaphysalis longicornis was the most frequently collected, representing 99.2% in Ulju, 98.9% in Gimhae. In the collection environments, a total number of 685, 268, 64, and 47 ticks were collected from a glassland, a copse, a mountain path, and a grave of Ulju a total number of 469, 216, 83, and 75 ticks were collected from a glassland, a copse, a Grave, and a mountain path of Gimhae respectively. In the results of the isolation of Severe Fever with Thrombocytopenia Syndrome (SFTS) from the ticks, no pathogens were detected from RNA of 101 pools (Ulju), 98 pools (Gimhae) of the ticks using a Polymerase Chain Reaction method in 2023.

왕피천 수계 내 저서성 대형무척추동물의 군집구조 분석을 위해 총 5개 지점에서 2023년 총 4회(4월, 6월, 8월 11월) 조사를 실시하였다. 조사기간 중 저서성 대형무척추동물은 총 5문 7강 17목 77과 156종 17,179.1개체/㎡가 채집되었다. 수환경 변화에 민감한 E.P.T. 분류군은 전체 156종 중 91종이 출현하여, 전체 출현종의 58.3%를 차지 하였다. 섭식기능군(FFGs) 분석결과, 종 출현 양상은 육식성 포식자(Predator: P)가 51종(32.69%)으로, 개체 출현 양상은 주워먹는 무리(Gathering-collector: GC)가 6,867.2개체/㎡(39.97%)로 높은 비율로 출현하였다. 서식기능 군(FHGs) 분석결과, 붙는 무리(Clinger: CL)가 70종(44.87%), 12,720.6개체/㎡(74.04%)로 가장 높은 비율로 출현 하였다. 군집지수 분석결과, 우점도지수(DI) 0.43, 다양도지수(H′) 3.51, 풍부도지수(R1) 4.59 균등도지수(J′) 0.77 로 나타났다. 생물학적 수질 판정 지수(BMI) 분석결과, 평균 92.36(±0.83)으로 모든 지점에서 “매우 좋음”으로 판정되었다.

This investigation was conducted three times (April, August, and October) from 2023 at five surveyed sites in Gonyangcheon estuarine wetland. A total of benthic macroinvertebrates belonging to 59 taxa 44 families 21 orders in 8 classes of 4 phyla were collected. The numbers of benthic macroinvertebrates species in each surveyed site were from 10 (St.1) to 41 (St.2). As a community indices analysis, the average DI is 0.78 (±0.12), H' is 1.6 (±0.68), R′ is 0.96 (±0.39), and J´ is 0.61 (±0.24). Community indices tended to decrease as the diversity and abundance decreased downstream. Total ecological score of benthic macroinvertebrates community (TESB), an average score of 35.47 (±15.86). The Habitat of Clithon retropictum and Ellobium chinense belonging to endangered species II, were identified at st.3~st.5.

In the Gucheon stream of Deogyusan National Park, a survey was conducted at three sites (six sites in total) in 2018 and 2021. A total of benthic macroinvertebrates of 107 taxa 46 families 13 orders in 6 classes of 5 phyla were found. According to the analysis of the appearance by year, a total of 87 species were surveyed in 2018, and a total of 89 species were surveyed in 2021. As a results to the functional feeding group analysis, Gathering-Collector (GC) accounted for a high species share (2018: 29.89%, 2021: 25.84%). In the functional habitat group analysis, Clinger (CL) accounted for a high species share (2018: 56.32%, 2021: 57.30%). Average annual community indices analysis, DI 0.38(±0.05), H′ 3.48(±0.19), R′ 3.23(±0.41), and J′ 0.85(±0.04) in 2018, and DI 0.55(±0.09), H′ 2.98(±0.31), R′ 2.91(±0.65), and J′ 0.70(±0.05) in 2021. As a results of the benthic macroinvertebrates index (BMI) analysis, indicating that all sites were classified as "very good," with average BMI values of 95.24(±2.84) in 2018 and 96.99(±1.13) in 2021.

본 연구는 소양강댐 하류에서 서식하는 생태계교란 생물 종인 브라운송어와 그 먹이원으로 이용되는 저서성 대형무척추동물에 대한 파악을 위해 2022년부터 2023년까지 총 8회에 걸쳐 소양강댐 하류(St.1~St.3)와 지류 (St.4)에 대해 브라운송어와 공서종, 브라운송어의 위 내용물, 저서성 대형무척추동물의 종조성 및 기능군 분석을 실시하였다. 저서성 대형무척추동물의 경우, 하루살이목에서 가장 많은 분류군이 확인되었으며(27.1%), 그 중 붙는 무리(CL)와 헤엄치는 무리(SW)가 높은 비율을 차지하는 것으로 확인되었다. 브라운송어 채집 결과, 전장은 26∼246mm까지 총 105개체가 채집되었으며, 전장-체중 관계의 매개변수 b값이 3을 초과하여 안정적인 성장이 이루어지는 것으로 확인되었다. 브라운송어의 위 내용물에 대한 먹이원 분석 결과, 빙어(0.2%, TL: 246mm)와 육상곤충(2.7%, TL: 154mm, 183∼185mm)을 섭식한 개체는 매우 적었으며 상대적으로 전장이 큰 개체에서 확인 되었다. 대부분 수서곤충(73.8%)과 물 속에서 서식하는 비곤충류(23.3%)를 섭식하는 것으로 나타났다. 브라운 송어의 전장에 따른 먹이 섭식 패턴을 파악하기 위해 위 내용물에서 확인된 종들과의 상관분석을 실시한 결과, 브라운송어의 먹이원 중 유수성 환경 선호 종들의 경우 전장과 양의 상관관계(p<0.05)를 나타낸 반면, 모래 기질 이하의 흐름이 적은 서식처를 선호하는 종들의 경우 전장과 음의 상관관계(p<0.05)를 나타냈다.

An unrecorded gall midge was discovered from inflorescence galls on Castanopsis sieboldii (Makino) Hatus. ex T.Yamaz. & Mashiba, which is one of major components in evergreen forests on the Korean Peninsula. The galls occurred on 20 trees out of 230 on Yokiji Island. The gall midge was identified as Schizomyia castanopsisae Elsayed & Tokuda, 2018 (Diptera: Cecidomyiidae), using morphological characters and mitochondrial DNA cytochrome oxidase subunit 1 (COI) region sequences of gall midge’s larvae. The Barcode sequences of 40 samples collected from Yokji Island were identical, and the individuals from Yokji Island formed a clade with the individuals from Kyushu, with robust bootstrap support in a maximum likelihood tree. This result suggests the gall midges may have migrated from Kyushu, Japan to Yokji Island, South Korea. However, it is too early to determine if the gall midge is truly invasive or not at present due to paucity of distribution data in the country.

국내 원예작물에서 화분매개곤충의 의존도는 해마다 증가하고 있다. 우리는 사과, 딸기 등 화분매개곤충의 의존도가 높은 주요 과수와 시설작물에 대하여 화분매개곤충의 사용기술을 개발하고 이를 현장에 적용하는 연구를 수행하고 있다. 2021년부터 2022년까지 딸기, 키위 등 5가지 주요 농작물에서 화분매개벌을 적용한 결과, 딸기에서 뒤영벌의 적용결과 기존 꿀벌과 통계적으로 같은 수준의 화분매개효과를 나타내었으며, 하우스에서 농약적용시 하우스 밖으로 벌통을 위치시키는 것이 방치보다 벌의 소실을 20% 더 감소시킬 수 있었다. 인공수분 에 의존하고 있는 씨없는 수박에서 수분수 식재와 꿀벌을 이용함으로 기존 벌 방사보다 16% 착과율을 향상시킬 수 있었다. 시설고추에서 꿀벌과 뒤영벌의 혼합사용시 기존 꿀벌 방사보다 고추 수량이 10% 향상되었고, 토마토 에서 660㎡당 뒤영벌의 봉군량을 1.5배 증가시 토마토 수량은 4.4% 향상되었다. 키위는 꿀벌로 기존의 인공수분 을 충분히 대체할 수 있었고, 인건비가 60%이상 절감되었다. 또한 현재 시판중인 30종의 살충제와 27종의 살균제 에 대하여 24시간내 반수치사를 보인 농약은 각각 살충제 6종와 살균제 4종 이었다. 이어서 2023년부터 참외, 멜론, 사과, 단감에 대한 현장적용연구가 진행되고 있다.

Bee traffic at the hive entrance can be used as an important indicator of foraging activity. We investigated patterns of honeybees and bumblebees near their hives as a basis for calculating bee traffic using the image deep learning. The flight pattern near the hive differed significantly according to bee at entering and leaving the hive. Honeybees mainly showed flight that changed flight direction more than once (69.5%), whereas bumblebees mainly performed straight flight (48.7%) or had a single turn (36.5%) in flight. When bees entered the hive, honeybees primarily showed one-turn or two-turn flight patterns(88.5%), and bumblebees showed a one-turn flight pattern (48.0%). In contrast, when leaving the hive, honeybees primarily showed a straight flight pattern (63.0%), and bumblebees primarily showed a straight or one-turn pattern (90.5%). There was a significant difference in flight speed according to the flight pattern. The speed of straight flight (0.89±0.47 m/s) was 1.5 to 2.1 times faster than flight where direction changed. Therefore, our results can help determine the capturing and recognizing the flying image of bees when calculating bee traffic by image deep learning.

본 연구는 소리곤충 상담 프로그램이 노인의 정서표현에 도움을 주는지를 확인하고자 하였다. 연구대상은 남녀 구분 없이 만 65세 이상이며 정서표현 척도 검사에서 51점 이하, 의사소통에 문제가 없는 자로 실험집단 10명 통제집단 10명을 선정하였다. 프로그램은 2023년 7월부터 8월까지 주 2회, 회기당 50분씩 총 12회기를 실시 하였다. 효과 검증을 위해 SPSS/WIN 26.0을 활용하여 집단 간 동질성 검증을 하고 입증이 된 후 실험집단과 통제집 단의 사전과 사후에 노인 정서표현 척도 측정을 실시하고 분석하였다. 그 결과, 실험집단의 정서표현 전체의 사전 평균 56.25에서 사후평균 66.38로 유의미한 차이가 나타났다(Z=-3.41, p＜.05). 그러나 통제집단의 정서표현 전체의 사전평균 58.15에서 사후 평균 58.92로 유의미한 차이가 나타나지 않았다(Z=-0.43, p>.05). 향후 본 연구결 과를 기반으로 소리곤충을 활용한 노인의 정서표현 향상에 이점을 유도하는 소리곤충 활용 상담 프로그램으로 확대 적용할 수 있을 것으로 기대된다.

To identify viruses and compare their abundance levels in the venom glands of hymenopteran species, we conducted venom gland-specific transcriptome assemblies and analyses of 22 Aculeate bees and wasps and identified the RNA genomes of picornaviruses. Additionally, we investigated the expression patterns of viruses in the venom glands over time following capture. Honeybee-infecting viruses, including black queen cell virus (BQCV), deformed wing virus (DWV), and Israeli acute paralysis virus (IAPV), were highly expressed in the venom glands of Apis mellifera and social wasps. This finding suggests that the venoms of bees and wasps likely contain these viruses, which can be transmitted horizontally between species through their stinger usage. A. mellifera exhibited an increasing pattern of abundance levels for BQCV, DWV, IAPV, and Triatovirus, while the social wasp Vespa crabro showed increasing abundance levels of IAPV and Triatovirus over different capture periods. This suggests that the venom glands of honeybees and wasps may provide suitable conditions for active viral replication and may be an organ for virus accumulation and transmission. Some viral sequences clearly reflected the phylogeny of Aculeate species, implying host-specific virus evolution. On the other hand, other viruses exhibited unique evolutionary patterns of phylogeny, possibly caused by specific ecological interactions. Our study provides insights into the composition and evolutionary properties of viral genes in the venom glands of certain Aculeate bees and wasps, as well as the potential horizontal transmission of these viruses among bee and wasp species.

The two sibling species of fruit fly, Drosophila melanogaster and D. simulans (Diptera: Drosophilidae), have long been used as the key model organisms in ecological and evolutionary research. While numerous studies have investigated the thermal responses of these two species, no study has yet systematically compared their response to dietary macronutrient balance. To fill this knowledge gap, we compared how various life-history traits expressed during larval development would response to an array of dietary ratio of protein to carbohydrate (P:C ratio) in these two sibling species. Largely consistent with previous studies, D. melanogaster took longer to complete their larval development and were much larger at adult emergence than D. simulans. For both species, an increase in dietary P:C ratio resulted in improved larval survivorship and faster development. However, the two species showed qualitatively different response to dietary P:C ratio when body mass at adult eclosion was concerned. The body mass of D. melanogaster peaked at an optimal P:C ratio of 1:4, but decreased as the P:C ratio either increased or decreased from this optimum. In marked contrast, the body mass of D. simulans was insensitive to dietary P:C ratio.

먹노린재 합성집합페로몬 후보물질 12종에 대한 유인력을 평가하였다. 페로몬 방출기는 4구 후각계 (Olfacomter)를 사용하였고 4개의 진공관에 각각 후보물질을 투입 후 진공 유압 방식으로 방출하여 포집기에 유인되는 먹노린재의 개채수를 측정하는 방법으로 검정하였다. 4구 후각계 페로몬 평가 방법은 기존 Y-관 후각 계의 문제점인 양방향 선택성과 공간 한정성을 개선하여 평가의 정확성을 향상시켰다. 유인력 평가 결과 12종의 유인제 후보물질 중 Trans-2-Decenal이 먹노린재 실험개체에 대하여 100% 유인력을 보였다. 또한, 선별된 Trans-2-Decenal의 먹노린재 유인력에 대한 유효농도 시험을 진행 한 결과, 50%의 농도에서 유인력이 가장 높았 다. 본 연구를 통해서 선별된 Trans-2 Decenal은 기존의 노린재과에 대한 페로몬 트랩에 비해 먹노린재에 대한 유인 효과가 높을 것으로 사료되었다. 이에 따라, Trans-2 Decenal을 기반으로 한 페로몬 트랩이 상용화된다면 추후의 먹노린재 방제 효과가 높아질 것으로 기대된다.

노지 나방류 해충인 담배거세미나방 방제를 위한 유충기생성 천적 2종인 긴등기생파리와 예쁜가는배고치벌 의 방사조건을 확립하고자 콩 재배포장에서 방사거리 및 방사밀도별 기생율을 조사하였다. 긴등기생파리는 방사 후 24시간 이내 방사지점으로부터 10m까지 이동하여 나방류 유충을 기생하였으며, 예쁜가는배고치벌도 방사지점으로부터 15m에서도 기주유충에 산란하여 고치가 형성된 것이 확인되었다. 긴등기생파리는 면적당 방사밀도가 높아질수록 기생율이 증가했으며, ㎡당 0.15마리(천적:해충=1:5) 방사했을 때 평균기생율 63.1%로 나타났다. 예쁜가는배고치벌은 긴등기생파리와 같은 방사밀도에서 평균기생율 47.8%로 확인되었으나, 방사 밀도에 따른 기생율 차이는 없었다.

The ovicidal effect of plant extract mixture (5% cinnamon extract + 10% citronella oil + 30% citrus oil + 10% derris extract + 20% neem extract + 25% penetrating surfactant) against several major insect pests was tested using the spraying method. In the case of stink bugs, eggs tended to die during hatching. When treated with a plant extract mixture (500-times solution), mortality for Halyomorpha halys, Riptortus clavatus, Eurydema dominulus, Trialeurodes vaprarorium, Bemisia tabaci, Spodoptera exigua, and Agrotis ipsilon reached as high as 100%. Therefore, it is believed that industrialization will be feasible in the future.

To control western flower thrips (Frankliniella occidentalis), a significant pest of peppers, the control effects of eco-friendly agricultural materials were tested when treated alone and in combination. The control effects of yellow sticky roll traps installed on the stem, predatory natural enemy (Hypoaspis miles) inoculated on the stem, and plant extracts (50% custard apple seed oil + 10% cinnamon extract) sprayed on the foliage were 45.3%, 36.6%, and 50.2%, respectively, when treated individually. Meanwhile, when three eco-friendly agricultural materials were comprehensively applied, the control effect was maintained between 62.4% and 80.9% compared to untreated conditions during the pepper harvesting season. These methods can be utilized for the eco-friendly control of western flower thrips in pepper greenhouses.

M. pruinosa has been found on 98 families and 345 species of plants, indicating a wide host range. Since its first report in a persimmon orchard in Gimhae in 2009, it had spread to 126 municipalities over 12,429 ha. Because of powerful dispersal ability, it’s difficult to control with insecticides. Therefore, it is necessary to reduce the population density through the release of M. pruinosa’s natural enemy, N. typhlocybae. N. typhlocybae females prey on or externally parasitize the nymphs of M. pruinosa. This natural enemy was introduced from Italy in 2017 through an international cooperative project between National Institute of Agricultural Sciences and University of Padova for controlling the sporadic pest, Metcalfa pruinosa. This study reported the results of mass rearing N. typhlocybae indoors and releasing them outdoors from 2020 to 2023 in 21 regions nationwide, showing an average parasitism rate of 27.3% and an average production of 7.9 cocoons per host, suggesting the possibility of their establishment in Korea.

Native to southern Europe, Lepisiota frauenfeldi (Mayr, 1855) is an invasive species that has been introduced worldwide through cross-border trade. It has been documented to be distributed in the Mediterranean, Middle East, Africa, and Indo-Malaya. This species, also known as Browsing ant, was first found around a warehouse in Gwangyang, Jeollanam-do, South Korea, and was probably introduced into the country via cargo. The external morphology and gene sequences were checked for taxonomic identification, and it was identified as Lepisiota frauenfeldi based on comparative analysis of the morphological classification key and NCBI data. This study is the first report of a newly invasive alien ant and provides basic data on the species, including external morphological and ecological features and sequencing results.

Islands often have relatively well-preserved ecosystem and an abundance of bioresources with a high conservation value, with unrecorded species continuing to be reported (Hong, 2011). Approximately 1,000,000 species of insect known worldwide (Costello et al., 2012), and 20,710 species are known in Korea (NIBR, 2023). Among these, there are 6,117 species in Korean islands (HNIBR, 2022). Native insect Bio-scan project for Korean islands is to estimate the number of insect species on Korean islands. We attempted to estimate the number of insect species on Korean islands using Barcode Index Number (BIN), and also found unrecorded species. The samples were collected four times from April to July at five locations in the Amtedo, an island located in Shinan-gun Jeollanam-do. We tried to obtain a minimum of one to usually a maximum of four samples per morphospecies to enable DNA barcoding.

갈색거저리 유충의 사료인 밀기울은 대부분 수입에 의존하고 있는데 일부 국가의 식량 수출 중단 조치 등에 따른 국제 곡물가격 상승으로 밀기울 가격은 인상되고, 식용곤충 판매가격이 하락하면서 생산비 절감을 위한 사료 개발이 요구되고 있다. 농업부산물 3종을 50% 이상 급이하게 되면 유충 생육이 저하되었기 때문에 본 연구는 적정한 배합비율을 선정하기 위해 첨가사료 20, 30% 함량으로 밀기울과 혼합하여 사료를 급이하였을 때 갈색거 저리 유충의 생육 특성과 영양성분 변화에 대해 밀기울만 제공한 대조구와 비교하였다. 부산물 A와 B, C를 각각 30% 함유한 처리구에서 갈색거저리 유충 무게는 대조구와 차이가 없음을 확인하였다. 먹이소화율은 부산물B를 20% 함유한 처리구가 80.5%로 대조구에 비해 높았고, 부산물C 30% 처리구에서 72.6%로 가장 낮았다. 갈색거저 리 유충의 생육일수 100일 기준으로 부산물B 30% 처리구에서 용화율이 76.1%로 대조구보다 1.6배 높았으며, 부산물A 20% 처리구는 29.2%로 용화율이 가장 낮았다. 갈색거저리 유충의 조단백질 함량은 부산물 C 30% 처리 구에서 대조구보다 10.3% 증가하여 아미노산 분석을 진행한 결과 sarcosine과 ornithine이 2.5배 이상 증가하였다. 이를 통해 부산물 B나 C를 30% 함유한 사료를 급이하였을 때 사육 원가를 절감하여 유충을 생산할 수 있을 것으로 사료된다.